Báo cáo khoa học: The propeptide in the precursor form of carboxypeptidase Y ensures cooperative unfolding and the carbohydrate moiety exerts a protective effect against heat and pressure pot

... The propeptide in the precursor form of carboxypeptidase Y ensures cooperative unfolding and the carbohydrate moiety exerts a protective effect against heat and pressure Michiko Kato1, Yasuhiro ... vitro,aswellasformaintaining CPY in an inactive form [4–6]. The mature and precursor forms are glycoproteins [7], which contain % 16%carbohydrates [8]: the four carbohydrate chains are of similar ... ensuring cooperative unfolding. The carbohydrate moiety has a slightly protective role in heat- induced unfolding and a highly protective role in pressure- induced unfolding. Keywords: carboxypeptidase...

Báo cáo khoa học: Protein transport in organelles: Dual targeting of proteins to mitochondria and chloroplasts ppt

... proteinsTargeting signalsAnalysis of dual targeting signals indicates that theyare rather similar to plastidic and mitochondrial tar-geting signals, in that they are enriched in positivelycharged ... processingsite have a greater inhibitory affect of MPP compared toSPP. In the case of aminoacyl tRNA synthetases, Gluaminoacyl-tRNA synthetase was processed at the samesite in both mitochondria ... demonstrate that alternative translation istaking place in vivo. Analysis of the targeting ability of DNA polymerase c2 in another study revealed that itwas dual targeted, but from a protein produced...

Báo cáo khoa học: "Centrality Measures in Text Mining: Prediction of Noun " docx

... centrality instead of degree centrality. IC: same as DC except for using information cen-trality instead of degree centrality. FQ: including Frequency and all other non-centrality features. The ... actors. A social network is composed of a set of actors and the rela-tion or relations defined on them (Wasserman and Faust, 1994). Graph theory has been used in social network analysis to ... the summary. Here we can adopt the same way of asking the user to provide a limit for the NPs in the summary. We used the actual number of NPs the author used in his/her abstract in our experiment....

Báo cáo khoa học: Thiamin diphosphate in biological chemistry: analogues of thiamin diphosphate in studies of enzymes and riboswitches pot

... enzymesthrough the use of ThDP analogues. Many such analogues have been syn-thesized and have provided information on the functional groups necessaryfor the binding and catalytic activity of the cofactor. ... and the ordering of the C-terminus of the protein to form a lid over the CoAchain (PDB entry 2JI6) [34]. In the case of phenylpyru-vate decarboxylase, crystallizing the enzymes with3-deazaThDP ... dimethylamino group, its important role in catalysis soon became apparent [9]. These analoguesgenerally showed good binding capacity but were cata-lytically inactive, offering further proof of the...

Báo cáo khoa học: C-terminal, endoplasmic reticulum-lumenal domain of prosurfactant protein C – structural features and membrane interactions ppt

... 95 °C at a heating rate of 0.5 °CÆmin)1. The reversibility of the thermal transition was evaluated byseveral cycles of heating and cooling. The standard micro-cal origin software was used ... cycle of heating and cooling, there was a distortion with the appear-ance of a low-temperature endotherm (Fig. 8A, scan 2). The heat capacity curves for the second and third scans overlapped. Interestingly, ... 547C-terminal, endoplasmic reticulum-lumenal domain of prosurfactant protein C – structural features and membrane interactionsCristina Casals1, Hanna Johansson2, Alejandra Saenz1, Magnus Gustafsson2,3,...

Tài liệu Báo cáo khoa học: Brain angiogenesis in developmental and pathological processes: regulation, molecular and cellular communication at the neurovascular interface pdf

... in ammatoryresponses [85]. In addition, the slight edema of astro-cytes helps maintain the water balance in the brain,but pathologic cytotoxic edema is a main cause of increased intracranial pressure ... that astrocytes play active roles in various brainfunctions. In particular, astrocytes function as adultneural stem cells, participate in the formation and mod-ulation of synapses, and in the ... materials via their high buf-fering capacity; however, if astrocytes reach the state of reactive gliosis, they are also capable of releasingcytokines that disrupt the BBB and elicit in ammatoryresponses...

Tài liệu Báo cáo khoa học: Nuclear receptors in the mosquito Aedes aegypti Annotation, hormonal regulation and expression proﬁling ppt

... fat bodies were collected and RNA lev-els were analyzed using qPCR. Transcript abundance values for AaEcRA, AaEcRB, AaUSP -A, AaUSP-B, AaE7 5A (A) , AaHR3, AaHR4, AaE78,AaHR39, AaHR78 (B) and AabFTZ-F 1A, ... biological repli-cates were analyzed, and Fig. 2 depicts the proﬁlematching both replicates.An increase in transcript abundance for AaEcRA,AaEcRB, AaE7 5A, AaE75B, AaE75C, AaHR3,AaHR4, AaE78 and ... genes AaE7 5A, AaHR3, AaHR4, AaE78 (B), AaHR39, AaHR78(C), the competence factor AabFTZ-F1 isoforms A and B (C), the hepatocyte nuclear factor isoforms AaHNF- 4A, AaHNF-4B , AaHNF-4C (D), the non-20E-responsive...

Tài liệu Báo cáo khoa học: Electrostatic contacts in the activator protein-1 coiled coil enhance stability predominantly by decreasing the unfolding rate docx

... kcalÆmol)1 of increased stability). This wasevident in the folding pathway for the Arg-Glu mutantvia both a slightly faster folding rate and a vastlydecelerated overall unfolding rate, relative to the ... dissociation is fun-damental in lead design and synthesis of peptide-basedantagonists that aim to bind and sequester proteinsthat are behaving abnormally. Often, the most rationalplace to begin ... of the identiﬁable states of the folding pathway and relate to the amount of solvent-exposed surface area in each of these states (see Materials and methods). This can bedone for all ﬁve states...

Tài liệu Báo cáo khoa học: Verprolin function in endocytosis and actin organization Roles of the Las17p (yeast WASP)-binding domain and a novel C-terminal actin-binding domain doc

... WASP)-binding domain and a novelC-terminal actin-binding domainThirumaran Thanabalu1,2, Rajamuthiah Rajmohan2, Lei Meng2, Gang Ren4,5, Parimala R. Vajjhala4 and Alan L. Munn1,3,4,6*1 Institute ... ﬁlament assemblymachinery or whether it has a more general role in stabilizing or altering protein conformation and thatregulation of the actin-ﬁlament assembly machinery isonly one example of ... consistentwith a role of F-actin and ⁄ or actin polymerization in the generation or maintenance of a polarized distribu-tion of cortical patches.Las17p and type I myosins promote the assembly of actin...

Tài liệu Báo cáo khoa học: Fermentative lifestyle in yeasts belonging to the Saccharomyces complex ppt

... due to amino acid biosynthesis. Much of the generation of NADH during amino acid biosyn-thesis takes place in the mitochondria. Because of the block in the respiratory chain caused by the addition of ... subdivided intopre- and post-genome duplication lineages. Appar-ently, the duplication took place after the separation of Saccharomyces, Kazachstania, Naumovia, Nakesimia and Tetrapisispora from the ... grow after 7 days on bothcomplex and synthetic minimal media (Fig. 1). All an-alyzed species belonging to the Saccharomyces, Kaz-achstania, Naumovia, Nakaseomyces and Tetrapisisporagenera were...

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