Báo cáo khoa học: Molecular cloning, expression analysis and functional confirmation of ecdysone receptor and ultraspiracle from the Colorado potato beetle Leptinotarsa decemlineata pdf

Báo cáo khoa học: Molecular cloning, expression analysis and functional confirmation of ecdysone receptor and ultraspiracle from the Colorado potato beetle Leptinotarsa decemlineata pdf

Báo cáo khoa học: Molecular cloning, expression analysis and functional confirmation of ecdysone receptor and ultraspiracle from the Colorado potato beetle Leptinotarsa decemlineata pdf

... 4114–4128 ª 2005 FEBS Molecular cloning, expression analysis and functional confirmation of ecdysone receptor and ultraspiracle from the Colorado potato beetle Leptinotarsa decemlineata Takehiko ... ecdysone receptor (EcR) and ultraspiracle (USP) of the coleopteran Colorado potato beetle Leptinotarsa decemlineata (LdEcR and LdUSP) w...
Ngày tải lên : 07/03/2014, 21:20
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Báo cáo khoa học: Molecular cloning, expression and characterization of protein disulfide isomerase from Conus marmoreus pdf

Báo cáo khoa học: Molecular cloning, expression and characterization of protein disulfide isomerase from Conus marmoreus pdf

... rate of decrease of the linear form, the original data were fitted by Y(t) ¼ e –kt · 100%, where Y is the percentage of the linear form, and t is the refolding time. For the rate of increase of the ... experiments. For the rate of increase of the native form in the presence of cPDI, the original data were fitted by Y(t) ¼ Y max (1 ) e –kt ) · 100%, where...
Ngày tải lên : 07/03/2014, 05:20
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Báo cáo khoa học: Molecular cloning, expression and characterization of cDNA encoding cis-prenyltransferases from Hevea brasiliensis A key factor participating in natural rubber biosynthesis pdf

Báo cáo khoa học: Molecular cloning, expression and characterization of cDNA encoding cis-prenyltransferases from Hevea brasiliensis A key factor participating in natural rubber biosynthesis pdf

... PCR at either the 5¢-end or 3¢-end of the coding regions of the two HRT cDNAs. The resulting fragments were sequenced, digested with NcoIandBamHI, and ligated into the NcoI–BamHI vector of pET32b(+), ... pET32b(+), yielding the expression plasmids pETHRT1 and pETHRT2. Each of the expression plasmids was used for transformation of E. coli BL21(DE3), and 1 mL...
Ngày tải lên : 07/03/2014, 21:20
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Tài liệu Báo cáo khoa học: Molecular cloning, recombinant expression and IgE-binding epitope of x-5 gliadin, a major allergen in wheat-dependent exercise-induced anaphylaxis ppt

Tài liệu Báo cáo khoa học: Molecular cloning, recombinant expression and IgE-binding epitope of x-5 gliadin, a major allergen in wheat-dependent exercise-induced anaphylaxis ppt

... region of the x-5 gliadin gene, PCR primers were designed at the position of the initi- ation and termination codons of the gene based on the nucleotide sequences extracted from a database of wheat expressed ... WDEIA. We cloned and determined the nucleotide sequence of two x-5 gliadin genes, x-5a and x-5b, from geno- mic DNA of wheat cultivar Norin 61, a Jap...
Ngày tải lên : 20/02/2014, 01:20
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Báo cáo khoa học: Molecular cloning of the Matrix Gla Protein gene from Xenopus laevis Functional analysis of the promoter identifies a calcium sensitive region required for basal activity doc

Báo cáo khoa học: Molecular cloning of the Matrix Gla Protein gene from Xenopus laevis Functional analysis of the promoter identifies a calcium sensitive region required for basal activity doc

... two exons (IA and IB) in the X. laevis gene, with the site of the intron insertion localized within the 5¢ UTR region of t he xMGP gene (Fig. 1 and Table 2 ). The other introns (2, 3 and 4) are ... conserved sites within the MGP coding sequence (Fig. 2 ). Analysis of the phase of each of the xMGP introns located within the coding region revealed that intron...
Ngày tải lên : 08/03/2014, 10:20
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Báo cáo khoa học: Molecular cloning and functional expression of the human sodium channel b1B subunit, a novel splicing variant of the b1 subunit potx

Báo cáo khoa học: Molecular cloning and functional expression of the human sodium channel b1B subunit, a novel splicing variant of the b1 subunit potx

... in the presence or absence of the b 1B subunit, whereas, under the same conditions, the rate of inactivation was increased in the presence of the b 1 subunit. The effects of b 1B were further assessed ... where G max is the maximum conductance, z is the valence of the process and V ½ is the midpoint voltage of activation. Ionic current expression levels w...
Ngày tải lên : 16/03/2014, 23:20
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Báo cáo khoa học: Molecular cloning and characterization of the crustacean hyperglycemic hormone cDNA from Litopenaeus schmitti Functional analysis by double-stranded RNA interference technique pot

Báo cáo khoa học: Molecular cloning and characterization of the crustacean hyperglycemic hormone cDNA from Litopenaeus schmitti Functional analysis by double-stranded RNA interference technique pot

... of the sinus gland and the one which gives the name to the family [3]. The main CHH activity is to elevate glucose concen- tration in the hemolymph by a process of glycogen de- gradation in the ... cysteine residues at the same positions as that of other CHHs of penaeid shrimps (Fig. 1B). Tissue-specific gene expression of L. schmitti CHH gene The size and ex...
Ngày tải lên : 23/03/2014, 10:20
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Báo cáo khoa học: Molecular cloning and functional expression of a gene encoding an antiarrhythmia peptide derived from the scorpion toxin pptx

Báo cáo khoa học: Molecular cloning and functional expression of a gene encoding an antiarrhythmia peptide derived from the scorpion toxin pptx

... work, we describe the cloning of the gene sequence of BmKIM, the functional expression of the recombinant toxin in Escheri- chia coli and its effect on the sodium channels of neurons and ventricular ... obtained was the same as the cDNA. Therefore, there is only one intron in the sequence encoding the signal peptide. The position and structure of BmKIM...
Ngày tải lên : 31/03/2014, 09:20
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Tài liệu Báo cáo khoa học: Molecular cloning and characterization of soybean protein disulfide isomerase family proteins with nonclassic active center motifs pdf

Tài liệu Báo cáo khoa học: Molecular cloning and characterization of soybean protein disulfide isomerase family proteins with nonclassic active center motifs pdf

... organelle and membrane proteins are synthe- sized and folded with the assistance of molecular chap- erones and other folding factors in the endoplasmic reticulum (ER). In many cases, the process of ... of the 53 kDa spot (5.3) was identical to a pI value calcu- lated from the amino acid sequence of GmPDIL-3a, and the isoelectric point of the 57 kDa spot (5....
Ngày tải lên : 18/02/2014, 11:20
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Báo cáo khoa học: Molecular cloning and characterization of two soybean protein disulfide isomerases as molecular chaperones for seed storage proteins doc

Báo cáo khoa học: Molecular cloning and characterization of two soybean protein disulfide isomerases as molecular chaperones for seed storage proteins doc

... expression were found in the promoter regions of both GmPDIL-1 and GmPDIL-2. However, the mRNA expression patterns of GmPDIL-1 and GmPDIL-2 were different, suggesting that the expression of these ... b-conglycinin during the course of the folding process of these proteins. Results Cloning and expression of GmPDIL-1 and GmPDIL-2 To clone the soybean orth...
Ngày tải lên : 07/03/2014, 05:20
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