Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx

... FEBS Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis Melissa C. Miles1, Michelle L. Janket1, ... regulation of caspases. Takentogether, these results indicate that VBARP is a novel splice variant of ANKHD1 and may play a role in cellular apoptosis (antiapoptotic) and cell survival pathway(s).AbbreviationsANK, ... blast searches of VBARP revealed that thisprotein has homology to human ankyrin repeat and KH domain containing 1 (ANKHD1) variants, and toan unknown protein named PP2500 [7,8]. AlthoughANKHD1...

Báo cáo khoa học: Alpha 1-antichymotrypsin/SerpinA3 is a novel target of orphan nuclear receptor Nur77 potx

... primerswere 5¢-ATGGTGGGAATGGGTCAGAAG-3¢ and 5¢-CACGCAGCTCATTGTAGAAGG-3¢. Another primer pairused for SerpinA3 was ACTas5-ACTas3: 5¢-GAATCCACCAGCTACATCCA-3¢ and 5¢-GTGCCCTCCTCAAATACATCA-3¢.Western ... 5¢-gacGGATCCgcagtccagccatgctcctttcaagagaaggagcatg-3¢ (with BamHI), 5¢-cggAAGCTTtATCGATccaaaaaacagtccagccatgctccttctcttg-3¢ (with HindIII and ClaI) with 16 complementary bases, which were denatured,annealed ... to asTR3 or NGFI-B) is a member of the NR 4A family, and is composed of three typical functional domains:an N-terminal region (transactivation function 1,AF-1), a DNA-binding domain, and a ligand-binding domain. ...

Báo cáo khoa học: Escherichia coli cyclophilin B binds a highly distorted form of trans-prolyl peptide isomer doc

... planarity of the Ala-cis-Pro amidebond. In the case of CyPB in complexes with Suc-Ala-trans-Pro-Ala-pNA and Ac-Ala-Ala-trans-Pro-Ala-AMC, the largedistortion in the orientation of Ala1 and Ala2 ... Ala-Pro, Ala-Pro-Phe, Ala-Ala-Pro-Ala and Ala-Ala-Pro-Ala-Ala i nhibit cis to transisomerase activity of calf thymus CyPA [27]. It wassuggested that in the mechanism of the observed isomeri-zation ... (Suc-Ala-Pro-Ala-pNA), and w ith a tetrapeptide, acetyl-Ala-Ala-Pro-Ala-amidomethylcouma-rin (Ac-Ala-Ala-Pro-Ala-AMC), and w e found highlydistorted trans-peptides. We carried out comparisons of these...

Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc

... proteins. The prim–pol domain and putative heli-case ⁄ NTPase domain are indicated in gray and black respectively.(B) Purification of the recombinant Rep245 protein. SDS ⁄ PAGE of protein extracts ... the DNA binding and the active site subdomains, we man-aged to model the entire domain without it.Another significant finding concerns the nature of the acid residues within the active site of ... in the reaction mixture,this reaction was clearly template dependent and specific. The fact that the Rep245 variant retained the capabil-ity of the RepA full-length protein of synthesizing and elongating...

Tài liệu Báo cáo khoa học: Molecular and functional characterization of adenylate kinase 2 gene from Leishmania donovani pdf

... USAATP-regenerating enzymes may have an important role in maintaining ATP levels in mitochondria-like kinetoplastorganelle and glycosomes in parasitic protozoa. Adenylatekinase (AK) (ATP:AMP ... wereobtained by simple rotation of the same model. The flexible LID domain appears at top of the three figures, NMP-binding site and ATPbinding domain are displayed, NH2- and COOH-termini are alsoindicated.Ó ... immunizedrabbits. A single band of 26 kDa was detected, as shown in Fig. 4B. Functional characterization of recombinant AK The recombinant protein purified from the inclusionbodies and refolded in the...

Báo cáo khoa học: Molecular and functional characterization of novel CRFR1 isoforms from the skin pptx

... P764(5¢-AACTCGAGGCTAGTCTGCAGGAGCTCAAGCTTTCTAGAGAATTCA-3¢)andP765(5¢-TGAATTCTCTAGAAAGCTTGAGCTCCTGCAGACTAGCCTCGAGTT-3¢). It was also digested with EcoRI and XhoI, ligatedwiththevectorandclonedinJM109Escherichia ... to adenylate cyclase with the exception of an artiﬁcial isoform (CRFR1h2) with the insertion of 37 amino acids between the ligand binding domain and the ﬁrst extracellular loop that was capable ... 2004 Molecular and functional characterization of novel CRFR1 isoformsfrom the skinAlexander Pisarchik and Andrzej SlominskiDepartment of Pathology and Laboratory Medicine, University of Tennessee Health...

Báo cáo khoa học: Molecular cloning and characterization of the crustacean hyperglycemic hormone cDNA from Litopenaeus schmitti Functional analysis by double-stranded RNA interference technique pot

... than six CHH-likecDNA have been identified and can be divided intoCHH -A and CHH-B groups [19]. In the crabs Can-cer pagurus, Carcinus maenas and Libinia emarginata and in the crayfishes Procambarus ... RNAs were allowed to anneal bymixing equal amounts of each strand, heating to 100 °C for1 min, and cooling gradually to room temperature for3–4 h. Single-stranded RNAs and the annealed RNA(dsRNA) ... compared against b-actinprobe and the b-actin transcript was observed as a defined band in the all eyestalk total RNA sample tes-ted (Fig. 5B).Similar results were observed in the semiquantita-tive...

Tài liệu Báo cáo khoa học: Molecular characterization of Arabidopsis thaliana PUF proteins – binding speciﬁcity and target candidates doc

... cucaucucuccuuacaguuuaccuguguaggaguuaggguucuugaauaaacaaugcaacaaagauuguagaagucagUGUACAUAAt4g36040 (1) Protein containing DNAJ domain; unknown functioncuacgucggacggaacugggaaaccgaucaguguugguagugaguuaacucggugaccgaguuaguagaacgaguuaauuagUGUAAAUAcgaagccaAt4g39090 ... 5¢-GAGCCAACAGAAGTTTGCTTCACACGTTGTTGAGAAATGTTT-3¢ (forward) and 5¢-GTCAAACATTTCTCAACAACGTGTGAAGCAAACTTCTGTTGG-3¢ (reverse) to APUM-2 and 5¢-CGATGCAGAAATTCAGTAGCAACATGGTGGAACGATGTCTCA-3¢ (forward) ... containing PHD domain; unknown functionugcgucugacaUGUACAGCcccugccaaauuuuaauaggcaatAGUAAAUAaauaacgacaagaagcaaauggAt5g24490 (1) Ribosomal protein; unknown function cucaucucuccuuacaguuuaccuguguaggaguuaggguucuugaauaaacaaugcaacaaagauuguagaagucagUGUACAUAAt4g36040...

Tài liệu Báo cáo khoa học: Molecular cloning and characterization of soybean protein disulﬁde isomerase family proteins with nonclassic active center motifs pdf

... within the a- domain and a -domain. Instead, they both contain the sequence CPRS in the a- domain and CMNC or CINC in the a -domain. GmPDIL- 3a and GmPDIL-3b contain a C-terminalKDEL sequence that ... with a Thermal Cycler Dice RealTime System (TaKaRa Bio Inc.). Forward primers 5¢-CGTTTGAAGGGTGAGGAGGAAAA[FAM]G-3¢ and 5¢-CACAAGAGAGTTCTGCGATAACCTTG[FAM]G-3¢ (Invi-trogen Corporation) and reverse ... werereplaced with AGG. (D) Putative domain structure of GmPDIL- 3a and GmPDIL-3b. The boxes indicate the domain boundariespredicted by an NCBI conserved domain search. Black boxes in domain -a and domain -a ...

Tài liệu Báo cáo khoa học: Molecular and biochemical characterization ofD-phosphoglycerate dehydrogenase fromEntamoeba histolytica A unique enteric protozoan parasite that possesses both phosphorylated and nonphosphorylated serine metabolic pathways docx

... subunits have been implicated at the nucleotide binding domains and the regulatory domain, indicating the importance of these domains for the tetramerization of the enzyme [18]. It was shown that serine ... betweenthedomainsaredepictedby An open box in the nucleotide binding domain indicates the NAD+-binding domain (Gly-Xaa-Gly-Xaa2-Gly-Xaa17-Asp) and all conserved residues implicated for the NAD+binding are inverted ... dataindicate, by analogy, that serine biosynthesis may also beregulated to maintain the intracellular cysteine concentra-tion in the amoeba. Modulation of expression of PGDH and other enzymes involved...

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