Báo cáo khoa học: Death inducer obliterator protein 1 in the context of DNA regulation Sequence analyses of distant homologues point to a novel functional role docx

... HYPOTHESIS Death inducer obliterator protein 1 in the context of DNA regulation Sequence analyses of distant homologues point to a novel functional role Ana M. Rojas 1, 2, Luis Sanchez-Pulido 1 , ... noteworthy that the retrievedproteins all appear to have a role in DNA regulation, in the context of chromatin stability, and to form part of higher complexes linked to active chromatin. The DIDO1 gene ... the minimal transcrip-tionally active fragment and are required simulta-neously to maintain transcription. The PHF3 protein was recovered in initial analyses and also contains a TFS2M domain...

Báo cáo khoa học: Prevalent conformations and subunit exchange in the biologically active apoptin protein multimer potx

... nm.When MBP–apoptin-IA was combined with MBP–apoptin-FM in NaCl/Piata10:1molarratioofincorporated label and incubated at 30 °C, we observed a clear increase in FFM/FIAover the course of 24 ... IA and FM and combined at the same label ratio. (B) IA ﬁ FM FRET-based exchange assay. MBP–apoptin-IA and MBP–apoptin-FM were combined at an IA to FM ratio of 10 : 1 and incubated at 30 °C for ... [15 ,16 ]. The emission spectra of MBP–apoptin-IA and MBP–apoptin-NBD were symmetrical and had a half-maximal peak widthcomparable to that of the free 2-mercaptoethanol adductFig. 2. The majority of...

Báo cáo khoa học: Biochemical evidence for conformational changes in the cross-talk between adenylation and peptidyl-carrier protein domains of nonribosomal peptide synthetases ppt

... simultaneously into the catalytic centre of the A domain awaiting amino acyltransfer. To adopt this conformation, the PCP domainhas to dock on the A domain in an orientationwhereby the Ppant attachment ... domains of the tyrocidine and bacitracinNRPS. Cys3 31 is part of the binding pocket of the amino acid in the active site [11 ]. The mutationCys331Leu decreased the activity of the isolated GrsA A ... foran A domain and also with the idea that binding of the PCP to the A domain in a productive mannerincreases the compactness of the protein. Further control reactions with a noncognate inhibi-tor...

Tài liệu Báo cáo khoa học: Hypothalamic malonyl-CoA and CPT1c in the treatment of obesity pptx

... in various organ systems. Even in organismslacking a brain, such as Caenorhabditis elegans, the nervous system plays a key role in maintaining energybalance [1 4]. In more advanced, mammalian ... synthaseactivates PPARalpha to maintain energy homeostasis.J Clin Invest 11 7, 2539–2552. 19 Hu Z, Cha SH, Chohnan S & Lane MD (2003) Hypo-thalamic malonyl-CoA as a mediator of feeding behav-ior. ... novofatty acid synthesis is the carboxylation of acetyl-CoA to form malonyl-CoA, catalyzed by ACC – the key reg-ulatory enzyme in the pathway. Malonyl-CoA serves as the basic chain-elongating substrate...

Tài liệu Báo cáo khoa học: "Toward finer-grained sentiment identification in product reviews through linguistic and ontological analyses" ppt

... level of detail. If the customer wants to comment on some attributes in detail, she could use a fine-grained concept (e.g., the width of the thigh part of the pants) rather than a coarse-grained ... manually. We obtained raw text reviews from one of the major online shopping malls in Korea 1 and per-formed a morphology analysis and POS-tagging. After POS-tagging, we collected all the ... that may affect such fine-grained po-larity. We argue that relevance for satisfaction, contrastive weight clues, and certain adver-bials work to affect the polarity, as evidenced by the statistical...

Tài liệu Báo cáo khoa học: "Subgrammars, Rule Classes and Control in the Rosetta Translation System" ppt

... One of its 12 1 disadvantages is that it leads to a proliferation of rather unnatural categories. It is hard to find an elegant and transparent way of specifying rule order in a compositional ... individual grammars may be com- plicated because of the attuning. • Principle of Interllnguality: There is an intermediate language into which analysis com- ponents of various languages translate ... approach can be applied in a compositional grammar, in an insightful and linguistically moti- vated way. Lack of control on rule applications In many cases the grammar writer has a certain...

Báo cáo khoa học: Amyloid oligomers: dynamics and toxicity in the cytosol and nucleus ppt

... expansion in ataxin-3 is the cause of the most common dominantataxia, spinocerebellar ataxia type 3 (SCA3, alsoknown as Machado–Joseph disease). SCA2, SCA6,SCA7 and SCA17 are also caused by ... that of the rapidlyexchanging inclusions. Inverse FRAP analysis of ataxin- 1 indicated that wild-type ataxin -1 shuttles between the nucleus and the cytosol, whereas polyQ-expandedataxin -1 is ... damaged by a negativechain reaction. This situation can be explained byescape from (or collapse of) the protein homeostasis net-work [13 ,14 ], and the accumulating incapacitation of protein...

Báo cáo khoa học: Insulin induces heme oxygenase-1 through the phosphatidylinositol 3-kinase/Akt pathway and the Nrf2 transcription factor in renal cells pptx

... small-interfering RNA abolishedinsulin-induced heme oxygenase -1 induction. Insulin was also found to acti-vate the mitogen-activated protein kinase cascades p38 and extracellularsignal-related ... Normoxic induction of the hypoxia-induciblefactor 1alpha by insulin and interleukin-1beta involves the phosphatidylinositol 3-kinase pathway. FEBS Lett 512 , 15 7 16 2.53 Fukuda R, Hirota K, Fan F, ... based on the pUSEamp vector. The activated form (Akt-myr) containsan N-terminal myristoylation sequence targeting Akt to the plasma membrane. The dominant-negative form(Akt-K179M) contains a methionine...

Báo cáo khoa học: "Parsing Free Word Order Languages in the Paninian Framework" pptx

... (19 76), (19 88)). See Fig. 1. Two of the important karakas are karta karaka and karma karaka. Frequently, the karta karaka maps to agent theta role, and the karma to theme or goal theta role. ... karaka of 'le' (take) is the karana (instru- mental) karaka of 'kAta' (cut). G.I rAma Pala KAkara mohana ko bulAtA hE. Ram fruit having-eaten Mohan -ko calls (Having eaten ... verb. 10 9 bulA (call) kar/ ~arma~rec ede rAma mohana KA (eat) karta.~5 ~arma rAma Pala (~ruit) KA (eat) rAma Pala kAta (cut) (fruit) karta . karma rAma Pala le (take) kart/~arma~~urpos...

Báo cáo khoa học: Monomeric molten globule intermediate involved in the equilibrium unfolding of tetrameric duck d2-crystallin pdf

... refraction [1 3]. Thermodynamic stabilityfor crystallins is essential in maintaining lens transparency[4]. Determining the mechanism of folding and assembly of these proteins is important for ... disturbanceFig. 6. Diagram of the surface of duck d-crystallin (1AUWÆpdb). (A) Each subunit of the tetrameric d-crystallin shows a surface with several convexand concave areas as indicated by a , ... association. The active site is located at a boundary composed of three subunits [8 11 ].Identifying and characterizing of possible conformationalstates in the pathways leading to folding and...

Xem thêm