Báo cáo khoa học: Pharmacology of vascular endothelium Delivered on 27 June 2004 at the 29th FEBS Congress in Warsaw pptx

... hydrophilicpravastatin) at a concentration of 30 lm mobilize freecytoplasmic calcium [Ca2+]ito 30–50% of thatinduced by Bk at a concentration of 10 nm. In the case of simvastatin and lovastatin, this ... [59]. In Langendorff’s preparation of guinea-pig heart, statins produce NO•-dependent vaso-dilatation of coronary vascular bed [59]. The precon-tracted bovine coronary artery rings with endothelium are ... endothelial dysfunction in patients withatherosclerosis [8]. The great expectations for the therapeutic use of antioxidants in patients with athero-sclerosis need to be re-examined.Endothelium...

Báo cáo khoa học: How a lipid mediates tumour suppression Delivered on 29 June 2010 at the 35th FEBS Congress in Gothenburg, Sweden pdf

... domain-containing proteins and some45 PX domain-containing proteins that presumablymediate most (but not all) of the downstream functions of PtdIns3P [29]. Additional PtdIns3P-binding proteinsthat ... certain variant pleckstrin homology domains suchas the GLUE (GRAM-like ubiquitin-binding in EAP45) domain [32–34].Intracellular localization of PtdIns3P The identiﬁcation of PtdIns3P-binding ... deubiquitinat-ed by deubiquitinating enzymes recruited by ESCRT-III[61], and ESCRT-III serves to sever the neck of the forming invagination, thereby securing the inclusion of cargo proteins within...

Tài liệu Báo cáo khoa học: Effect of siRNA terminal mismatches on TRBP and Dicer binding and silencing efﬁcacy pdf

... studied the relationship between the silencing efﬁciency of the siRNAs and theirbinding to the RNA-induced silencing complex loading complex proteinsHIV transactivating response RNA-binding protein and ... function of TRBP in RNAi.Here, we have characterized the interactions of siRNAs that contain terminal mismatches with TRBPand Dicer, and determined the impact of these interac-tions on their ... Simultaneous single or doublemismatches at both ends of the duplex signiﬁcantlyreduced binding by TRBP, echoing what was seen withmismatches at only one end. As with one terminalmismatch, binding by...

Tài liệu Báo cáo khoa học: Analysis of proteins and peptides on a chromatographic timescale by electron-transfer dissociation MS ppt

... 274 (2007) 6269– 6276 ª 2007 The Authors Journal compilation ª 2007 FEBS 6271 dimethylation of Arg, monomethylation, dimethylationand trimethylation of Lys, acetylation and ubiquitina-tion of ... phosphorylation of Ser and Thr [16–18]. Combinations of these modifications are suggestedto constitute a ‘histone code’ that regulates the binding of protein complexes involved in transcription, replica-tion, ... characterizing intact proteins in mix-tures on a chromatographic timescale.Identification of PTMsOne of the first research applications demonstrating the value of ETD involves strategies employed...

Tài liệu Báo cáo khoa học: His374 of wheat endoxylanase inhibitor TAXI-I stabilizes complex formation with glycoside hydrolase family 11 endoxylanases pptx

... xylose. In spite of the above, profound analysis of the speci-ﬁc contributions of the amino acid residues at the interface of the enzyme–inhibitor interaction is lacking.Mutagenesis studies of a ... of the interaction with B. subtilisendoxylanase than the k on rate constants of the inter-actions with A. niger or T. longibrachiatum M2endoxylanases. At the same time, the increase in koffvalues ... TAXI[H374Q](Table 2). The side chain of Gln (containing one nitro-gen atom) probably accounts for additional hydrogenbonding interactions compared to the aliphatic sidechain of Ala, resulting in stronger...

Báo cáo khoa học: Analysis of DNA-binding sites on Mhr1, a yeast mitochondrial ATP-independent homologous pairing protein potx

... compilation ª 2010 FEBS 1443 the reduction in the efﬁciency of FRET in the presence of the W71F or W165A mutants was not due to defects in the DNA-binding activities of these mutants.DNA-binding ... RIKEN SPring-8 Center, Hyogo, JapanIntroductionHomologous DNA recombination is conserved in allorganisms. In the nucleus, homologous recombinationis involved in the maintenance of genome integrityduring ... [37–39]. The ﬁrst RecA-like domain, which ispositioned at the N-terminus of dnRad54, contains aputative ATP-binding site (WalkerA motif); however, the second RecA-like domain, positioned at the...

Báo cáo khoa học: Modulation of nitric oxide-mediated metal release from metallothionein by the redox state of glutathione in vitro doc

... combination of tighter binding of cadmium than zinc in metallothioneinsand the preference of zinc in the more flexible b-domain. In addition to the already described differences in flexibility of the ... of 100 : 1 and only insignificant amounts of cadmium beingreleased at the highest ONOO–concentration. Even moreinterestingly, in contrast to the peroxynitrite-mediatedactivation of the guanylyl ... because the oxi-dation state of thiols is involved in the metal binding in Cd-Sor Zn-S clusters found in metallothioneins, and NO alsobinds to reduced glutathione via S-nitrosation. Using avariety...

Báo cáo khoa học: Regulation of arginase II by interferon regulatory factor 3 and the involvement of polyamines in the antiviral response potx

... observed in Jurkat cells in response to SeV infection.ArgII is involved in the biosynthesis of natural poly-amines (putrescine, spermidine and spermine) throughconversion of l-arginine into l-ornithine ... NF-jBinArgII regulation. Our study is thus the ﬁrst directdemonstration of the involvement of IRF-3 in ArgIIregulation in response to virus infection. IRF-3 is alsoactivated in response to LPS in a TLR-4-dependentmechanism ... mitochondrial enzymeinvolved in the polyamine synthesis pathway through the catalysis of l-ornithine production from l-arginine. Of the natural polyamines, spermine and to a lesserextent spermidine,...

Tài liệu Báo cáo khoa học: Development of a new method for isolation and long-term culture of organ-speciﬁc blood vascular and lymphatic endothelial cells of the mouse pdf

... aliquot of the transgene was introduced into E14.1 ESCs by electro-poration. ESCs expressing the transgene were selected byincubation for 7 days in medium containing a concentra-tion of G418 (Invitrogen) ... compilation ª 2008 FEBS 1991substantial proportion of the cell population from the uterus (Fig. 3C) also stained positive for Lyve-1, indi-cating that cell populations obtained from these tissueswere ... then antigen-retrieved for the detection of SV40T Ag by incubation in NaCl ⁄ Picontaining 0.1–0.25% trypsin and 0.5 mm EDTA at 37 °C or room tem-perature for 10–25 min. Prior to incubation...

Báo cáo khoa học: "Impact of computerized physician order entry on medication prescription errors in the intensive care unit: a controlled cross-sectional trial"

... the unnoticed changing of an already activated prescription of acontinuous infusion medication. Since recent upgrading of the system every continuous infusion prescription changebecomes immediately visible ... notation was properly filled in 69% of the patients in the C-U, whereas only 2% had an allergy notation in the charts of the PB-U. The only allergy error we encountered was in the C-U in a patient ... MPEs. Dose, dosing error; Rate, wrong infusion rate of continuous medica-tion; Route, wrong route of administration; Name, error in drug name; Interaction, drug-drug interaction error; Allergy,...

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