Báo cáo khoa học: Interaction of human stefin B in the prefibrillar oligomeric form with membranes Correlation with cellular toxicity doc
Báo cáo khoa học: Interaction of human stefin B in the prefibrillar oligomeric form with membranes Correlation with cellular toxicity doc
... confirm the binding of < /b> the aggregated forms,albeit to a much smaller extent than that observed forsome proteins that bind specifically to membranes, such as the small membrane-binding domains involved in ... a correlation among amyloidfibril formation, interaction < /b> with phospholipids, and cellular toxicity, using a model amyloidogenic protein, human < /b> stefin < /b> B. Stefin < /b> B is a member of < /b> the I25 family of < /b> ... [17,18]. By following the kinet-ics of < /b> fibril formation, conditions were defined in which the protein exists in the form of < /b> prefibrillar oligomers ⁄ aggregates, which persist during the lagphase. These...
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Tài liệu Báo cáo khoa học: Glycation of low-density lipoprotein results in the time-dependent accumulation of cholesteryl esters and apolipoprotein B-100 protein in primary human monocyte-derived macrophages docx
... resulting in the accu-mulation of < /b> both unmodified cholesteryl esters andglycated apoB in the cells. The rate of < /b> removal of < /b> the latter species is slow, with only 20–30% of < /b> the glycatedprotein being ... the turnover of < /b> preaccu-mulated protein to be studied in the absence of < /b> further cellular uptake. In these studies, cell death was < 12%as measured by the appearance of < /b> nondegraded apoB in ... lipoproteins in diabetes. Diabetes Metab Rev12, 69–90.10 Tomkin GH & Owens D (2001) Abnormalities in apo B- containing lipoproteins in diabetes and atherosclero-sis. Diabetes Metab Rev 17,...
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Báo cáo khoa học: Interaction of synthetic peptides corresponding to hepatitis G virus (HGV/GBV-C) E2 structural protein with phospholipid vesicles doc
... of < /b> the interaction < /b> of< /b> viral fusion proteins with membranes and the mechan-ism by which these proteins accelerate the formation of < /b> membrane fusion intermediates are poorly under-stood [2]. In ... thatdetermine peptide–membrane interaction,< /b> and com-bined analysis of < /b> the data obtained provides insightsinto HGV ⁄ GBV-C–membrane interaction.< /b> Results The E2 peptides synthesized are amphiphilic because of < /b> ... neither the hydrophobicity (0.16) nor the pI(6.14) of < /b> the parent E2(279–298) peptide.Binding of < /b> E2 peptides to model membranes Lipid interaction < /b> of < /b> the E2 peptides was studied bymonitoring...
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Báo cáo khoa học: Interaction of ostreolysin, a cytolytic protein from the edible mushroom Pleurotus ostreatus, with lipid membranes and modulation by lysophospholipids pptx
... either 0 ° or 90 ° (with respect to the plane of < /b> reflections). In the case of < /b> lipid-bound protein, the spectrum of < /b> the protein was obtained by subtracting the contribution of < /b> the lipid alone, with ... lyso-PtdInsfor 20 min before adding the LUVs or preincubating LUVsand then adding ostreolysin.Fluorescence measurementsSteady-state intrinsic fluorescence of < /b> ostreolysin, eitheralone or in combination ... (1985) Studies on toxin of< /b> Aspergillus fumigatus. XXII. Fashion of < /b> binding of < /b> Asp-hemolysinto human < /b> erythrocytes and Asp-hemolysin-binding proteins of< /b> erythrocyte membranes. Microbiol. Immunol. 29,...
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Tài liệu Báo cáo khoa học: Roles of AP-2 transcription factors in the regulation of cartilage and skeletal development doc
... diseases[47].Most analyses of < /b> the regulation of < /b> AP-2 and the interactions of < /b> the transcription factor with bindingpartners, as well as of < /b> the regulation of < /b> target geneexpression, have been performed for AP-2a.Uptonow, ... differentiatedphenotype.For AP- 2b, expression in murine limbs has also beendemonstrated. AP- 2b is expressed in the zone of < /b> polar-izing activity (ZPA), the signaling center of < /b> the devel-oping vertebrate limb [68]. ... [73]. The authors of < /b> this studyascribe the regulation of < /b> outgrowth of < /b> limb buds andpatterning of < /b> the digits to the chicken AP-2. The role of < /b> AP-2a was further studied in zebrafish.It was confirmed...
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Báo cáo khoa học: Analysis of Lsm1p and Lsm8p domains in the cellular localization of Lsm complexes in budding yeast ppt
... toproper U6 binding, either directly or indirectly, byaffecting the RNA-binding ability of < /b> the resultingheteroheptameric Lsm complex (see Lsm8DC, Lsm818,Lsm881 and Lsm188). Minimal U6 binding byGFPGFPIPInputLsm7-MycU6 ... the latter, indicating that in the absence of < /b> an N-terminaldomain distinct localization is lacking. Finally, the Lsm1p Sm domain by itself (LsmDN1DCp) accumu-lates in both the nucleus and the ... (Fig. 6A). Thus, in the absence of < /b> the C-terminal domain, the N-terminal domain, not the Sm domain, determines the subcellular localization. Bycontrast, LsmDN18p and LsmDN81p both shownuclear...
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Báo cáo khoa học: Kinetics of enzyme acylation and deacylation in the penicillin acylase-catalyzed synthesis of b-lactam antibiotics pptx
... nucleophilicattack of < /b> the active-site serine, bS1, on the carbonyl carbon of < /b> the amide or ester bond of < /b> the substrate [3] (residues arelabelled to indicate the subunit (a or b) and their position in the subunit). ... acid may bind in the acyl-binding site of < /b> PA. Hydrolysis of < /b> p-NPA may thus proceedvia reversed binding of < /b> the substrate, in which the leavinggroup, p-nitrophenol, occupies the acyl binding site ... to the acyl-enzyme lowers the kh2morethan binding of < /b> 6-APA. To discriminate between these twophenomena we studied binding of < /b> 6-APA and 7-ADCA to the enzyme by measuring the inhibition of < /b> the...
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Báo cáo khoa học: Role of peroxidase inhibition by insulin in the bovine thyroid cell proliferation mechanism pdf
... described above. The control activity of< /b> TPO was determined in the presence of < /b> inactivated alkalinephosphatase.Action of < /b> a-mannosidase on the inhibitor The inhibitor purified as described above, ... notshown). The absence of < /b> lipids in the structure of < /b> the peroxidaseinhibitor, discernible throughout the purification process,was confirmed by the fact that all the inhibitory activityremained in the ... insulinmay be explained by a dual action on both [24]. Besides, ithas been identified hybrids formed by subunits of < /b> the insulinand the IGF I receptors [23].Table 2. Comparative effects of < /b> the...
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Báo cáo khoa học: Interaction of G-rich GT oligonucleotides with nuclearassociated eEF1A is correlated with their antiproliferative effect in haematopoietic human cancer cell lines potx
... to the presence of < /b> a high molar excess of < /b> BSA remaining in the recovery buffer. Therefore, a fixed aliquot of < /b> the proteinwas incubated with 1 ng of < /b> the indicated probes. After30 min incubation at ... form with it the 100-kDa complex[21]. Figure 3B shows the binding of < /b> labelled oligomersto the nuclear-enriched fraction of < /b> proteins. The effi-ciency of < /b> labelling of < /b> the single oligomers being ... com-patible with the binding of < /b> eEF1A in the CRC (whitearrow), the other of < /b> apparent molecular mass of < /b> about100 kDa compatible with binding to nucleolin (blackarrow). The competition experiments in...
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Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt
... by splicing,whereas a dashed line in exon 7 of < /b> fragment C represents a dele-tion of < /b> 39 bp. The thick lines indicate intron 6 of < /b> 75 bp in fragments B and C, 3¢ portion of < /b> 35 bp in intron 5 in ... of < /b> alternative promoters in DNASE1 results in no generation of < /b> protein isoforms. The 5¢-UTR of < /b> eukaryotic mRNA in uences the initi-ation step of < /b> protein synthesis and thereby in partdetermines ... up-shifted band when the probewas incubated with the nuclear extracts (lanes 1 and7). Formation of < /b> the up-shifted complex, indicated by the arrow, was decreased by the addition of < /b> compet-ing unlabeled...
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Từ khóa: báo cáo khoa họcbáo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcbáo cáo khoa học nông nghiệpbáo cáo khoa học lâm nghiệpBáo cáo quy trình mua hàng CT CP Công Nghệ NPVNghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt namMột số giải pháp nâng cao chất lượng streaming thích ứng video trên nền giao thức HTTPGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhTrả hồ sơ điều tra bổ sung đối với các tội xâm phạm sở hữu có tính chất chiếm đoạt theo pháp luật Tố tụng hình sự Việt Nam từ thực tiễn thành phố Hồ Chí Minh (Luận văn thạc sĩ)Phát hiện xâm nhập dựa trên thuật toán k meansNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Sở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXTổ chức và hoạt động của Phòng Tư pháp từ thực tiễn tỉnh Phú Thọ (Luận văn thạc sĩ)Kiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)BT Tieng anh 6 UNIT 2Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIChiến lược marketing tại ngân hàng Agribank chi nhánh Sài Gòn từ 2013-2015TÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ