Báo cáo khoa học: Solution structure and backbone dynamics of the XPC-binding domain of the human DNA repair protein hHR23B docx

Tài liệu Báo cáo khoa học: Solution structure of hirsutellin A – new insights into the active site and interacting interfaces of ribotoxins docx

... Superposition of the active-siteresidues of the 20 conformers of the solu-tion structure of HtA. Catalytic groups E66 and H113 are shown in red, and side chains of other residues in their vicinity ... quality and precision of the 20 structures aresummarized in Table 1, and a superposition and generalview of the structures is shown in Fig. 2A,B. The meanpairwise rmsd value is 0.92 A˚for the backbone ... interactions to understand the efﬁcient cytotoxic activity of HtA. The contributions of the N-terminalhairpin, loop 2 and loop 5 with regard to protein functionality, protein protein and protein lipid...

Tài liệu Báo cáo khoa học: Design, structure and biological activity of b-turn peptides of CD2 protein for inhibition of T-cell adhesion ppt

... at the N- and C-termini. Acomparison of the b-turn structure of cER with the similarregion in the crystal structure of rat and human CD2 wascarried out. In the rat CD2 crystal structure, the ... structure in solution and clo-sely mimic the b-turn s tructure of the surface epitopes of the CD2 protein. Docking studies of CD2 peptides and CD58 protein revealed the possible binding sites of the ... with the higher inhibitory activity of the cEL peptide and the verylow inhibitory activity of the cYT peptide compared toother peptides. The ﬂanking residue of the b-turn, i.e. Tyr3 of the cVYpeptide...

Tài liệu Báo cáo khoa học: Solution structure of crotamine, a Na+ channel affecting toxin from Crotalus durissus terriﬁcus venom docx

... toxin structure and potential surface The characteristic NOE connectivities, reported in Fig. 3A, and the analysis of the / and w angle distribution allowed the identiﬁcation of the type and boundaries ... [47] of the triple-stranded antiparallel b-sheet wasFig. 3. Summary of data used in the determination of the secondary structure of crotamine. (A) Primary sequence of Crt with the sequential and medium-range ... details of the statistics of the NMR models are listed in Table 1.Analysis of the Ramachandran plot, performed with the PROCHECK_NMRprogram [31], shows that 64% of the residues lie in the most...

Tài liệu Báo cáo khoa học: Solution structure of the matrix attachment region-binding domain of chicken MeCP2 ppt

... helix on the other face. It is thoughtthat the two inner strands of the b-sheet lie within the majorgroove of the DNA and that a hydrophobic pocket formedby the side chains of Y123 and I125 ... [15]. The solution structure of the MBD of rat (r)MeCP2 hasrecently been determined [17]. The MBD adopts a wedge-shaped structure, composed of an antiparallel b-sheet onone face of the wedge and ... contacts the methylgroups of methylated CpG. The DNA interface further-more contains several arginine and lysine side chainsforming hydrogen bonds with the bases and contacting the DNA backbone. The...

Báo cáo khoa học: Solution structure of the catalytic domain of RICH protein from goldﬁsh pot

... with the crystal structure of human CNPase cata-lytic domain (rmsd of 2.3 A˚over 205 residues).Sequence alignment of the catalytic domain of RICH and related proteins (Fig. 1B) identifies the ... close to itsFig. 2. Structure of goldfish RICH catalytic domain. (A) Stereo view of the backbone superposition of 10 lowest energy structures of the RICH catalytic domain. The superposition was ... Pro174–Glu208 and Leu233–Phe386. (B) Ribbon representation of the RICH catalytic domain. Secondary structure elements and the N- and C-termini are labeled. (C) Backbone overlay of catalytic domains of goldfish...

Tài liệu Báo cáo khoa học: Pronounced adipogenesis and increased insulin sensitivity caused by overproduction of prostaglandin D2 in vivo pptx

... obesity.Experimental proceduresGeneration of H-PGDS TG mice The coding region of human H-PGDS was cloned into the downstream sites of the chicken b-actin promoter and the CMV enhancer of the pCAGGS expression vector ... without fat, of WT and H-PGDS TG mice (n = 6). Continuousdissections of mouse fat and bone in the whole body were quantiﬁed by use of amicro-CT scanner and LATHETA software(Aloka). Open and closed ... Hayaishi O (2000) Structure and chromo-somal localization of human and mouse genes forhematopoietic prostaglandin D synthase. Conservation of the ancestral genomic structure of sigma-class gluta-thione...

Tài liệu Báo cáo khoa học: "NATURAL LANGUAGE AND COMPUTER INTEBFACE DESIGN MURRAY TUROFF DEPARTMENT OF COMPUTER AND IiVFORMATION SCIENCE IIEW JERSEY INSTITUTE OF TECHNOLOGY" ppt

... developing and evaluating the use of a computer as a direct aid to facilitating human communi- cation. The basic idea is to use the processing and logical capabilities of the computer to aid in the ... lines of text and the average comment or page is about 20 lines of text. EIES offers the user a complete set of differing interfaces including menus, commands, self-defined commands and ... they have had with the system. However, there is one key parameter which is insensitive to the degree, of experience or the rate of use of the system. This is the number of items a user receives...

Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf

... metal-binding siteat the interface of the two domains, which consists of four side chains: two (His31 and His79) from the N-terminal domain and two (Asp165 and His169) from the C-terminal domain (Fig. ... Mn-reconstitutedApeSOD, the metal ion was ﬁve-coordinate in trigonalbipyramidal geometry (Fig. 3A). Three of the ligands,OD2 of Asp165, NE2 of His79 and NE2 of His169,formed an equatorial plane. The other protein ... ligands in the Mn-reconstituted enzyme and an additional water oxy-gen, which, together with the OD2 of Asp165, the NE2 of His79 and the NE2 of His169, formed anequatorial plane (Fig. 3B). The metal...

Tài liệu Báo cáo khoa học: Solution NMR structure of an immunodominant epitope of myelin basic protein Conformational dependence on environment of an intrinsically unstructured protein doc

... proportional to the inverse of the average of the distance to the sixthpower (<r)6>), between the unpaired electron of the paramagnetic agent and the interacting nucleus. Thus,these agents ... along the horizontal axis wasused to convert the left structure to the right structure. The N-ter-minus is at the left for every structure. The best-ﬁt overlays of the 10 lowest overall energy structures ... (MBP) is one of the most abundant proteins in CNS myelin; MBP maintains the compaction of the sheath by anchoring the cytoplas-mic faces of the oligodendrocyte membranes [2], and is acandidate...

Tài liệu Báo cáo khoa học: Endotoxic activity and chemical structure of lipopolysaccharides from Chlamydia trachomatis serotypes E and L2 and Chlamydophila psittaci 6BC pdf

... 5 and 6) and assignment of all signals (Table 2) revealed that the carbohydrate structure of the major component of C. tra-chomatis E LPS consisted of three Kdo- and two GlcpN-residues. The 1H-NMR ... have compared the endotoxic activities of three chlamydial LPS of known structure and deﬁned purity. Whereas the chemical struc-tures of the LPS from C. trachomatis serotype L2 and of Chl. psittaci ... 2005) and of laser-induced fragment ions [18] representing the lipid A moiety of LPS (centered around m/z 1850, 1641, and 1347). The mass difference of D m/z 660 between the respective groups of...

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