Báo cáo khoa học: Determination of thioxylo-oligosaccharide binding to family 11 xylanases using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and X-ray crystallography pot

Báo cáo khoa học: Determination of thioxylo-oligosaccharide binding to family 11 xylanases using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and X-ray crystallography pot

Báo cáo khoa học: Determination of thioxylo-oligosaccharide binding to family 11 xylanases using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and X-ray crystallography pot

... Calcium and peptide binding to folded and unfolded conformations of cardiac troponin C. Electro- spray ionization and Fourier transform ion cyclotron resonance mass spectrometry. Eur J Mass Spectrom ... al. Thioxylo-oligosaccharide binding to xylanases FEBS Journal 272 (2005) 2317–2333 ª 2005 FEBS 2333 Determination of thioxylo-oligosaccharide bindi...
Ngày tải lên : 07/03/2014, 17:20
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Báo cáo khoa học: Determination of the redox potentials and electron transfer properties of the FAD- and FMN-binding domains of the human oxidoreductase NR1 doc

Báo cáo khoa học: Determination of the redox potentials and electron transfer properties of the FAD- and FMN-binding domains of the human oxidoreductase NR1 doc

... the absence of cofactor reduction. Stopped-flow kinetic studies Reduction of the FAD/NADPH domain of NR1 was investigated by stopped-flow methods using a photodiode array detector. Aggregation of the ... units. To enable accurate determination of the FAD potentials, titrations of the NR1 FAD/NADPH domain were per- formed on samples of identical concentration over small range...
Ngày tải lên : 23/03/2014, 21:20
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Báo cáo khoa học: Characterization of heme-binding properties of Paracoccus denitrificans Surf1 proteins doc

Báo cáo khoa học: Characterization of heme-binding properties of Paracoccus denitrificans Surf1 proteins doc

... to water and couples the free energy of this reaction to the generation of a transmembrane proton gradient. The mitochondrial enzyme consists of up to 13 subun- its, of which only the key subunits ... group of heme a, and most likely presents the cofactor in a spatial orientation suitable for optimal transfer to its target site within subunit I of cytochrome c oxidase....
Ngày tải lên : 06/03/2014, 00:21
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Báo cáo khoa học: Analysis of DNA-binding sites on Mhr1, a yeast mitochondrial ATP-independent homologous pairing protein potx

Báo cáo khoa học: Analysis of DNA-binding sites on Mhr1, a yeast mitochondrial ATP-independent homologous pairing protein potx

... concen- tration was determined using an e 260 nm value of 7.0 · 10 3 m )1 Æcm )1 . Expression and purification of Mhr1 For the expression of recombinant Mhr1, E. coli BL21(DE3) pLysS DrecA cells were transformed ... recombina- tion of mtDNA in Saccharomyces cerevisiae. The mhr1-1 mutation causes defects in mtDNA duplication, partitioning to bud, and recovery of homoplasmy, al...
Ngày tải lên : 06/03/2014, 09:22
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Báo cáo khoa học: Characterization of HbpR binding by site-directed mutagenesis of its DNA-binding site and by deletion of the effector domain pot

Báo cáo khoa học: Characterization of HbpR binding by site-directed mutagenesis of its DNA-binding site and by deletion of the effector domain pot

... FEBS Characterization of HbpR binding by site-directed mutagenesis of its DNA -binding site and by deletion of the effector domain David Tropel 1 * and Jan R. van der Meer 1,2 1 Process of Environmental ... the HbpR–DNA binding interactions were investigated by site-directed mutagenesis of the operator region and by DNA -binding assays using purified HbpR. Mutations tha...
Ngày tải lên : 07/03/2014, 17:20
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Báo cáo khoa học: Determination of the reopening temperature of a DNA hairpin structure in vitro pptx

Báo cáo khoa học: Determination of the reopening temperature of a DNA hairpin structure in vitro pptx

... denaturation and renatur- ation manipulations, the fast annealing (fast renaturation) and slow annealing (slow renaturation) procedures (Materials and methods) were performed after the primer 2 and ... primer 2 and primer 3 under fast and slow annealing conditions. (A) Primer extension with primer 2 under slow and fast annealing conditions: lanes 1 and 2, primer extension prod...
Ngày tải lên : 23/03/2014, 13:20
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Báo cáo khoa học: " Determination of Syntactic Functions in Estonian Constraint Grammar " docx

Báo cáo khoa học: " Determination of Syntactic Functions in Estonian Constraint Grammar " docx

... of syntactic analysis of Estonian using Constraint Grammar. Constraint Gram- mar framework divides parsing into two different modules: morphological disam- biguation and determination of ... The process of syntactic analysis consists of three stages: mor- phological disambiguation, identification of clause boundaries, and identification of syntactic func- tions o...
Ngày tải lên : 24/03/2014, 03:21
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Báo cáo khóa học: Determination of modulation of ligand properties of synthetic complex-type biantennary N-glycans by introduction of bisecting GlcNAc in silico, in vitro and in vivo pot

Báo cáo khóa học: Determination of modulation of ligand properties of synthetic complex-type biantennary N-glycans by introduction of bisecting GlcNAc in silico, in vitro and in vivo pot

... human glioblastoma cell migration into the brain through modifications to the actin cytoskeleton and levels of expression of small GTPases. J. Neuropathol. Exp. Neurol. 61, 585–596. 134 S. Andre ´ et ... ligand properties of the neoglycoproteins, we determined their binding to cells and to tissue sections as well as their biodistribution in mice after injection of radioiodi...
Ngày tải lên : 30/03/2014, 13:20
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Báo cáo khoa học: Determination of the metal ion dependence and substrate specificity of a hydratase involved in the degradation pathway of biphenyl/chlorobiphenyl pot

Báo cáo khoa học: Determination of the metal ion dependence and substrate specificity of a hydratase involved in the degradation pathway of biphenyl/chlorobiphenyl pot

... substrate binding site and the role of the metal cofactor in the enzyme. Results Cloning, expression and purification of BphH The bphH gene was inserted into the expression vectors pT7-7 [11] , pEMBL18 ... values of 6.0 and 8.0 for catalysis. Number of metal ion bound per enzyme and determination of coordination number The equilibrium concentration of Mg 2+ bound to...
Ngày tải lên : 30/03/2014, 15:20
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Tài liệu Báo cáo khoa học: Bioinformatics of the glycoside hydrolase family 57 and identification of catalytic residues in amylopullulanase from Thermococcus hydrothermalis doc

Tài liệu Báo cáo khoa học: Bioinformatics of the glycoside hydrolase family 57 and identification of catalytic residues in amylopullulanase from Thermococcus hydrothermalis doc

... addition of 975 lLofNa 2 CO 3 (50 m M ). One unit (U) of activity was defined a s the quantity of enzyme necessary to release 1 lmol of 2-chloro-4-nitrophenol per min under the assay conditions, using ... concerning the conservation of the catalytic machinery [8]. Evolutionary relationships In order to draw the present-day evolutionary picture of the family GH-57, several e...
Ngày tải lên : 19/02/2014, 12:20
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