Báo cáo khoa học: Saccharomyces cerevisiae Ybr004c and its human homologue are required for addition of the second mannose during glycosylphosphatidylinositol precursor assembly ppt

... Saccharomyces cerevisiae Ybr004c and its human homologue are required for addition of the second mannose during glycosylphosphatidylinositol precursor assembly Anne-Lise Fabre1, ... protein(Ybr004cp) required for addition of the second man-nose to GPI precursors. Yeast cells depleted of Ybr004cp exhibit cell wall and morphological abnor-malities, are defective in the incorporation ... beenimplicated in all but one of the predicted reactions in the GPI pathway. The exception is the addition of the second mannose to the GPI core. We show here thatdepletion of the essential, multispanning...

Báo cáo khoa học: Copper-containing nitrite reductase fromPseudomonas chlororaphis DSM 50135 Evidence for modulation of the rate of intramolecular electron transfer through nitrite binding to the type 2 copper center pot

... and Shain, and plotted vs. the inverse of the scan rate. The plotsyielded straight lines, confirming the applicability of the Nicholson and Shain theory to the present system, and the variation of the ... CoCl2,0.6andNa2B2O7,0.1. The optical density, the concentrations of nitrate [17] and nitrite [18], the pO2 and the pH of the medium weremonitored, and the composition of the gaseous ... constant of the spectrum of the turnover form of the T2Cu center are slightly different from that of the as-purified form (cf.Table 2) which suggests the existence of modifications in the catalytic...

Báo cáo khoa học: Saccharomyces cerevisiae coq10 null mutants are responsive to antimycin A ppt

... show the pathway of reduction of coenzyme Q to the semiquinone at the P-site in the presence of antimycin A or my-xothiazol. The semiquinone formed in the presence of myxothiazolis the result of ... respectively, at the N-site and P-site of the Q-cycle [18–21]. Both inhibi-tors enhance the formation of oxygen radicals from the P-site [20,21]. Antimycin A binds to the N-site and blocks oxidation of cytochrome ... Aincreased the rate of H2O2release in wild-type and AOX transformants, but had no effect in the coq2 and bcs1 mutants over and above the rate seen without the inhibitor (Fig. 2B). The ability of...

Báo cáo khoa học: A steady-state modeling approach to validate an in vivo mechanism of the GAL regulatory network in Saccharomyces cerevisiae ppt

... togene expression. The status of the switch is determined by the state of the upstream regulatory element of the GALgenes in the nucleus. The regulatory protein may bind to the upstream regulatory ... is wellcharacterized at the biochemical and g enetic level. The switch constitutes elements for sensing of galactose and transduction of the measurement of galactose to the nucleus through protein–protein ... [8,9]. Another mechanismthat plays an important role in deciding the status of the switch is the distribution of the protein between the nucleus and cytoplasm. This is accomplished either bynucleocytoplasmic...

Báo cáo khoa học: Saccharomyces cerevisiae Pip2p–Oaf1p regulates PEX25 transcription through an adenine-less ORE ppt

... Pip2p–Oaf1p are markedwith arrows. Asterisks denote complexes of unknown identity. The reason for the increasein the intensity of the signal for the uppernonspeciﬁc complex (arrow and asterisk ... whether the difference between the modest induction level of PEX25 and the much higher one of PEX11 is due to the criticaldeviation from the consensus of the ORE in the formergene’s promoter, or ... structure of the ORE was alsoscrutinized, and we report on a new and signiﬁcantdeviation from the ORE consensus found in the promoter of PEX25.Experimental proceduresStrains and plasmids The S. cerevisiae...

Báo cáo khoa học: Saccharomyces cerevisiae a1,6-mannosyltransferase has a catalytic potential to transfer a second mannose molecule ppt

... results of a1,6-mannosi-dase resistance and the second mannose incorporationspeciﬁcities (Fig. 5). Consequently, the synthesis of product B was started by the addition of a mannose residue at the ... further understanding of the molecular mech-anism of the substrate recognition and enzymatic reac-tion, although the biological function of the second mannose addition of ScOch1p is still unknown. ... mole-cules of mannose to M9A acceptor.Structure of the novel product generatedby Och1pTo conﬁrm the position of incorporation of the novel second mannose, Man10GlcNAc2-PA and the novelproduct...

Báo cáo khoa học: Saccharomyces cerevisiae Cox18 complements the essential Sec-independent function of Escherichia coli YidC doc

... between the precursor and mature forms. In the presence of YidC, precursor M13P2 is converted into its matureform (Fig. 4B, upper panel, lane 1). In the absence of YidC, the processing of the precursor ... overexpression of the other [24]. The Oxa1 protein of Saccharomyces cerevisiae is the founding member of the YidC ⁄Oxa1 ⁄ Alb3 family, and was originally identiﬁed as afactor involved in the biogenesis of ... wasfound to be required for the efﬁcient topogenesis of Cox2, one of the central subunits of the cytochrome coxidase complex [34]. Cox18 ⁄ Oxa2 thereby directlyinteracts with Cox2 [24], but its molecular...

Tài liệu Báo cáo khoa học: "AUTOMATIC SPEECH RECOGNITION AND ITS APPLICATION TO INFORMATION EXTRACTION" pdf

... created. For the second input utterance, the likelihood value of the utterance given the speaker-independent model and that given the speaker-adapted model are calculated and compared. If the former ... and hardware. These advances include the widespread adoption of a statistical Figure 1 shows a mechanism of state -of- the- art speech recognizers [2]. Common features of these systems are the ... people, and organizations and all manner of relations among them. The great richness of material and the naturally evolving content in broadcast news has leveraged its value into areas of research...

Báo cáo khoa học: "Creating a CCGbank and a wide-coverage CCG lexicon for German" pdf

... tree. The algorithm starts at the rootcategory and recursively traverses the tree.NCLC L RRRH CC The CCG category of complements and of the root of the graph is determined from their ... CCG or TAG, and for the usefulness of de-pendency counts extracted from the training data.On the other hand, formalisms such as CCG and TAG a re particularly suited to capture the cross-ing ... posegreater challenges for syntactic theories (Rambow,1994), and the richer inﬂectional morphology of these languages creates additional problems both for the coverage of lexicalized formalisms suchas...

Báo cáo khóa học: Acyl-CoA dehydrogenases and acyl-CoA oxidases Structural basis for mechanistic similarities and differences pot

... interactions from the carbonyl of the substrate thioester to the 2¢OH of the ribityl chain of the FAD and to the main chain amide nitrogen of the residuecorresponding to Glu376 of MCAD. Therefore, in ... These interactions are important in the precisepositioning and alignment of the ﬂavin, substrate, and Glu376 for optimal catalysis. In addition, they are respon-sible for the acidiﬁcation of ... binding cavities for the fatty-acyl moiety of the substrates are shown. The surfaces of the ÔrimÕ of the cavities are very similar to that of MCAD shown inFig. 3. The orientation of each molecule...

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