Báo cáo khoa học: A ‘pure’ chemoattractant formylpeptide analogue triggers a speciﬁc signalling pathway in human neutrophil chemotaxis potx

... A ‘pure’ chemoattractant formylpeptide analogue triggers a speciﬁc signalling pathway in human neutrophil chemotaxis Susanna Spisani1, So a Falzarano1, Serena Traniello1, Marianna Nalli2and ... phosphatidylinositol-3-kinase, as well asactivating tyrosine phosphorylation. An increase in intracellular levels of cAMP [6,7] and the involvementof kinases, such as protein kinase C (PKC) and ... A2 -mediated signalling in neutrophils. Bio-chim Biophys Acta 1113, 135–160.9 Cui DY, Inanami O, Yamamori T, Niwa K, NagahataH & Kuwabara M (2000) FMLP-induced formation ofF-actin in HL60...

Tài liệu Báo cáo khoa học: DNA modiﬁcation with cisplatin affects sequence-speciﬁc DNA binding of p53 and p73 proteins in a target site-dependent manner pptx

... Katayama H, Sasai K, Kawai H, Yuan ZM, BondarukJ, Suzuki F, Fujii S, Arlinghaus RB, Czerniak BA &Sen S (2004) Phosphorylation by aurora kinase A induces Mdm2-mediated destabilization and ... anticancerdrug cisplatin. Nature 377, 649–652.29 Zlatanova J, Yaneva J & Leuba SH (1998) Proteins thatspeciﬁcally recognize cisplatin-damaged DNA: a clue toanticancer activity of cisplatin. FASEB J ... 5-ﬂuoro-uracil treatment. Int J Oncol 24, 115–125.56 Datta K, Shah P, Srivastava T, Mathur SG,Chattopadhyay P & Sinha S (2004) Sensitizing gliomacells to cisplatin by abrogating the p53...

Báo cáo khoa học: Effect of priming on activation and localization of phospholipase D-1 in human neutrophils potx

... oxidase is activated by PI3-kinase in the earlyphase and by PLD in a later phase. Brefeldin A, an inhibitorof ARF GTPases and therefore ARF activation, likewisecaused signiﬁcant inhibition ... (sense:5¢-ATGAGACACCCGGATCATGT; antisense: 5¢-ACTCACTGGACGGGTGAAAG; 496 bp product) andPLD2 (sense: 5¢-CTGCACCCCAACATAAAGGT; anti-sense: 3¢-GTTCTCCAGAGTCCCTGCTG; 594 bp prod-uct). For a control reaction, a speciﬁc ... kc220@cam.ac.ukAbbreviations:TNFa, tumour necrosis factor a; PAF, platelet acti-vating factor; PLD, phospholipase D; DAG, diacylglycerol; ARF,ADP-ribosylation factor; PI3-kinase, phosphatidylinositol...

Báo cáo khoa học: "Joint Identiﬁcation and Segmentation of Domain-Speciﬁc Dialogue Acts for Conversational Dialogue Systems" doc

... start from the same train-ing data as above, and create N copies, whereN is the number of unique dialogue acts labels in the training set. Each utterance-label pair in the original training ... present in all Ntraining sets. If in the original training set an ut-terance was labeled with the ithdialogue act la-bel, now it will be labeled as a positive example in the ithtraining set and ... set and as a negative exam-ple in all other training sets. Binary classiﬁersfor each N dialogue act labels are then trained.During run-time, each utterance is classiﬁed byall N models and the...

Báo cáo khoa học: Neuroprotective effects of naturally occurring polyphenols on quinolinic acid-induced excitotoxicity in human neurons ppt

... several important pathwaysinvolved in NOS-mediated neurotoxicity, includingactivation of nuclear factor kappa B, the Jun N-termi-nal kinase pathway and protein kinase C [26,44,45].Protein kinase ... curcumin, apigenin, naringenin and gallotanninwere obtained from Sigma-Aldrich (Castle-Hill, Australia).Phenazine methosulfate was obtained from ICN Biochem-icals (Aurora, OH, USA). Bradford reagent ... 2A) , PARP (Fig. 2B) and extracellular LDHactivities (Fig. 2C) for apigenin and naringenin.Effect of EPCG, catechin hydrate, curcumin,apigenin, naringenin and gallotannin onQUIN-mediated NAD+depletion,...

Báo cáo khoa học: Dissecting the role of protein–protein and protein–nucleic acid interactions in MS2 bacteriophage stability potx

... protein acts both as a translational repressorand as the principal viral structural protein, interactingspeciﬁcally with a 19-nucleotide RNA hairpin torepress translation of the viral replicase ... protein–protein and protein–nucleicacid interactions in MS2 bacteriophage stabilitySheila M. B. Lima1, Ana Carolina Q. Vaz1, Theo L. F. Souza1, David S. Peabody2, Jerson L. Silva1and Andre´ a ... protein may play a nucleationrole, inducing an aggregated state. A possible mechan-ism is the formation of a limited associated state, forexample a pentameric unit, as observed for other ani-mal...

Tài liệu Báo cáo khoa học: Neuronal growth-inhibitory factor (metallothionein-3): a unique metalloprotein ppt

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Tài liệu Báo cáo khoa học: Hypoxia downregulates farnesoid X receptor via a hypoxia-inducible factor-independent but p38 mitogen-activated protein kinase-dependent pathway doc

... thefollowing validated siRNAs: Hs_MAPK14_6_HP validatedsiRNA against human p3 8a; Hs_MAPK3_7_HP validatedsiRNA against human ERK1; and Hs_MAPK1_10_HPvalidated siRNA against human ERK2. HepG2 cells wereseeded ... cooled for annealing, and used to knock downHIF- 1a. Similarly, the siRNA against HIF- 2a was preparedusing the sense (5¢-CAGCAUCUUUGAUAGCAGUdTdT-3¢) and antisense (5¢-ACUGCUAUCAAAGAUGCUGdTdT-3¢) ... Fukumura D (2004) Hypoxia-induced activation of p38 mitogen-activated proteinkinase and phosphatidylinositol 3¢-kinase signalingpathways contributes to expression of interleukin 8 in human ovarian...

Tài liệu Báo cáo khoa học: Hypoxic resistance to articular chondrocyte apoptosis – a possible mechanism of maintaining homeostasis of normal articular cartilage pdf

... examination of thearticular cartilage after experimentally inducedOAArticular cartilage of the femoral condyles from theexperimental joints was examined to assess any macro-scopic damage caused ... proteasomeinhibition enhanced TRAIL-induced apoptosis in canine chondrocytes, we examined caspase-8 activationand death receptor-5 (DR-5) and TRAIL protein A BFig. 1. Evaluation of articular cartilage after ... ﬂuorescence after combinedtreatment. Photomicrographs indicated that ALLNand TRAIL alone induced a small change in MTP,whereas combined treatment with ALLN and TRAILcaused a signiﬁcant dissipation...

Tài liệu Báo cáo khoa học: Identiﬁcation, sequencing, and localization of a new carbonic anhydrase transcript from the hydrothermal vent tubeworm Riftia pachyptila docx

... GTT ACT TCC GCA GCT AGG 466–483Probe ampliﬁcation for FISHRpCAbrF TAC AAG GAT GCC ATT AGC 613–630RpCAbrR1 CGT AGC AGT ATC AGC AGT 822–839RpCAtrFprobe TAC AAA GAT CCA ATC CAG C 616–634RpCAtrRprobe ... Histidine residues involved in zinc binding in the catalytic site are indicated by a ‘Z’; important amino acids involved in the hydrogen bond network are indicated by anasterisk; framed amino acids ... and F. scutaria larvae sequences have a H64 also shared by A. gambiae, A. aegypti, T. gigas,D. melanogaster-2 and D. melanogaster-3 sequences(data not shown). By contrast, R. pachyptila aminoacid...

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