... with chloroform (20 mL), chloroform/methanol(98 : 2, v/v, 20 mL) and chloroform/methanol (1 : 3, v/v,25 mL) [16].In another experiment, total lipids from schizont formswere extracted and purified ... (pH 7.4). The mixture was extracted with chloroform/methanol (1 : 1) and analysed by TLC in chloroform/methanol/water (65 : 25 : 2, v/v/v). Lanes 1, 2 and 3 are rings, trophozoites and schizonts,respectively, ... fraction (II) , trihexosylceramide fraction (IV) and tetrahexosylcera-mide fraction (V) were extracted from the plate and analysed by UV-MALDI-TOF MS.Acid methanolysis and methylation The sample...