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Báo cáo khoa học: Effect of priming on activation and localization of phospholipase D-1 in human neutrophils potx

Báo cáo khoa học: Effect of priming on activation and localization of phospholipase D-1 in human neutrophils potx

Báo cáo khoa học: Effect of priming on activation and localization of phospholipase D-1 in human neutrophils potx

... N-formylmethionyl-leucyl-phenylalanine stimulation of human neutrophils. Biochem. J. 325,581–585.2764 K. A. Cadwallader et al.(Eur. J. Biochem. 271) Ó FEBS 2004 Effect of priming on activation and localization of phospholipase ... accumulating within the first5 min of stimulation. Effect of PI3-kinase and ARF inhibitors on OÀ2 and PLD activation Selective pharmacological inhibitors were used to assessthe role of PI3-kinase ... transfected with human PLD1 were used aspositive controls.The subcellular distribution of PLD and the conse-quences of priming and activation were determined usingimmunoprecipitation and Western...
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Tài liệu Báo cáo khoa học: The elusive intermediate on the folding pathway of the prion protein pptx

Tài liệu Báo cáo khoa học: The elusive intermediate on the folding pathway of the prion protein pptx

... typicalunfolding experiment, two stock solutions of PrP at identi-cal protein concentrations (5 lm) were prepared: one in buffer only (native protein) and one in buffer containing ahigh concentration of ... pro-tein should provide insight into the process of prionconversion, and represent a major step forward in ourunderstanding of the misfolding and aggregation of PrP during disease.Studies of ... °C on samples containing 5 lM protein.Fig. 8. Folding of PrP in health and disease. The folding mecha-nism of PrP incorporating the normal folding pathway (blue) pre-dominant in healthy conditions,...
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Tài liệu Báo cáo khoa học: Peptides corresponding to helices 5 and 6 of Bax can independently form large lipid pores pdf

Tài liệu Báo cáo khoa học: Peptides corresponding to helices 5 and 6 of Bax can independently form large lipid pores pdf

... is being formed, and has also been reportedfor the concerted action of Bax and tBid [10].Concluding remarks In summary, peptides corresponding to naturalsequences of Bax and encompassing individual ... curves, indicative of co-operativity, with in exion points corresponding to peptide concentra-tions giving 50% of maximum activity (IC50). WithLUVs made of PtdCho at 6 lm lipid concentration,IC50values ... HIIphasesunder conditions of reduced interlipid electrostaticrepulsion, as in the presence of Ca2+or polylysine pep-tides [33]. Similar conditions could be achieved throughthe binding of polycationic...
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Tài liệu Báo cáo khoa học: Arginine ethylester prevents thermal inactivation and aggregation of lysozyme pptx

Tài liệu Báo cáo khoa học: Arginine ethylester prevents thermal inactivation and aggregation of lysozyme pptx

... severaldifferent conditions are shown in Table 1. The rates o finactivation and aggregation d epend on the proteinFig. 1. Thermal inactivation and aggregation of lysozyme in the presence of additives. ... rate-limiting step in both inactivation and aggregation of lysozyme by heat treatment. As the d ata points werewell fitted to the single-exponential eq uation, the heat-induced aggregation and inactivation ... rates of inactivation and aggregation of lysozyme in the presence of ArgEE weresimilar to those in spermine, which is a favourable additiveto prevent t hermal inactivation and aggregation of l...
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Tài liệu Báo cáo khoa học: The undecided serpin The ins and outs of plasminogen activator inhibitor type 2 pdf

Tài liệu Báo cáo khoa học: The undecided serpin The ins and outs of plasminogen activator inhibitor type 2 pdf

... number of intracellular roles: itcan alter gene expression, in uence the rate of cell proliferation and differ-entiation, and inhibit apoptosis in a manner independent of urokinase inhi-bition. ... severalARE binding proteins, including the stabilizing proteinHuR [86] and the mRNA destabilizing protein tristetr-aprolin (TTP) [87]. HuR is a member of the Hu family of mRNA binding proteins and has ... role in eosinophil mediated in am-mation and tissue remodelling.Role of intranuclear PAI-2A number of Ov-serpins have been detected within thenuclear compartment, including bomapin, PI-9, and maspin...
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Báo cáo khoa học: Evolutionary changes to transthyretin: structure and function of a transthyretin-like ancestral protein doc

Báo cáo khoa học: Evolutionary changes to transthyretin: structure and function of a transthyretin-like ancestral protein doc

... functionalcharacterization of the TLP from various organisms, and the evolution of TLP and transthyretin.The identification of TLPs and transthyretins in natureThe evolution of transthyretin and ... involved in theoxidation of uric acid to allantoin. Certainly, theco -localization of these proteins in the peroxisomes of metazoan and plant species, and the co-regulation of TLP genes in some ... Evolution of the thyroidhormone-binding protein, transthyretin. Gen CompEndocrinol 119, 241–255.2 Richardson SJ (2007) Cell and molecular biology of transthyretin and thyroid hormones. Int Rev...
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Báo cáo khoa học: Post-ischemic brain damage: pathophysiology and role of inflammatory mediators ppt

Báo cáo khoa học: Post-ischemic brain damage: pathophysiology and role of inflammatory mediators ppt

... macrophages,suggesting that fractalkine is involved in neuron–microglia signalling [178]. In fact, this chemokineparticipates in leukocyte migration and in the activa-tion and chemoattraction of microglia into ... beneficial effects by promoting vasodilata-tion, whereas NO produced during later stages of injury by overactivation of neuronal NOS and de novoexpression of iNOS contributes to ischemic tissueinjury ... post-ischemic in ammationCellular response to injuryPost-ischemic in ammation is characterized by a rapid activation of resident microglial cells and by in ltra-tion of neutrophils and macrophages in...
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Báo cáo khoa học: pH dependence, substrate specificity and inhibition of human kynurenine aminotransferase I ppt

Báo cáo khoa học: pH dependence, substrate specificity and inhibition of human kynurenine aminotransferase I ppt

... aromatic amino acids (including,kynurenine, phenylalanine, tryptophan and tyrosine); sulfurcontaining amino a cids (including, methionine an d cysteine) and other aliphatic a mino acids (including, ... is efficient in catalyzing the transamination of anumber of amino acids, including glutamine, phenylalanine,leucine, kynurenine, methionine, tyrosine, histidine, cys-teine, and aminobutyrate ... KAT- I and II , have b eendescribed in r at and human brains [14–16]. In addition, KYNA is involved in maintaining physio-logical arterial blood pressure. In rats, the region of therostral and...
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Báo cáo khoa học: Cloning, over-expression, purification and characterization of Plasmodium falciparum enolase doc

Báo cáo khoa học: Cloning, over-expression, purification and characterization of Plasmodium falciparum enolase doc

... remains one of the most infectious diseases in thethird world with abou t 500 million infections and over onemillion deaths per year [1]. In the face of increasing threatsby resurgent infections ... recombinant protein and results in theaddition of a short noncleavable His tag sequence at itsN-terminus. Cloning resulted in incorporation of an a ddi-tional 18 (MRGSHHHHHHGSACELGT-) and seven(-LQPSLIS) ... to those of mammalian and yeast enolases.Figure 5B shows the effect of increasing concentrations of Mg2+ on the activity of r-Pfen, rabbit and yeast enolases. In the low concentration range,...
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Báo cáo khoa học: Systems biology: model based evaluation and comparison of potential explanations for given biological data pot

Báo cáo khoa học: Systems biology: model based evaluation and comparison of potential explanations for given biological data pot

... serving as conceptual intro-duction to the whole article.Introductory exampleThe example is concerned with insulin signaling, and isinspired by the developments in [12]. Insulin signalingoccurs ... example, in the evaluation of the importance of re-cycling of STAT5 [9–11], a pri-mary argument for the importance of this recyclingwas based on a model based analysis of the informa-tion contained ... vertical lines (which gives one standard deviation,with the mean in the middle) in Fig. 2. As is clearfrom the figure, the degree of phosphorylationincreases rapidly upon addition of insulin (100...
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