... pro-
pose a two- pathway electron transfer model for nitrate
reductase A from Escherichia coli.
Keywords: cytochrome b; electron transfer; Escherichia coli;
nitrate reductase A; quinone.
Nitrate can ... Evidence for two different electron transfer pathways in the same
enzyme, nitrate reductase A from
Escherichia coli
Roger Gi...
... was then dried and auto-
radiographed.
In the UV cross-linking assay, the samples were incuba-
ted at room temperature, as described above for the EMSA
assay, and then irradiated at 302 nm for ... and incubated with mAb for eEF 1A, as described in the
Materials and methods. Lane 1, bacterial recombinant eEF 1A protein
(R eEF 1A) ; lane 2, eEF 1A protein from total nuclea...
... indicates that the mechanism may be sim-
ilar to that of Co
2+
. The fact that the equilibrium con-
stants for the a domain are greater than those for the
b domain may be a factor in explaining ... K
3a
and K
4a
for the a
domain would have to be greater than K
2b
and K
3b
for
the b domain, to explain the observed filling of the
a domain prior to that of...
... gctttttggcaccaaagccctcggctccatcgg Lys24 to Ala
L2 5A L2 5A F gctttttggcaccaaaaaggccggctccatcg Leu25 to Ala
G3 3A G3 3A F ggttccgatcttgctgcgtcgatcaaagg Gly33 to Ala
F3 9A F3 9A F gcgtcgatcaaaggcgctaaaaaagcaatgagcg ... Ala
P2 6A P2 6A F cgcaacgactggctgtggcggtaaaaac Pro26 to Ala
L6 3A L6 3A F ggagtttcaggacagtgcgaaaaaggttgaaaagg Leu63 to Ala
2R > N R37N F gtagcgggctggattaacgcgttgaatt...
... colony forma-
tion assay. Table 2 shows the percentage of G418-
resistant colonies obtained by transfecting the established
human tumor pancreatic carcinoma MIA PaCa 2 and
the breast adenocarcinoma ... functions as a molecular switch in a large network
of signaling pathways [1]. Mutations in the ras gene have
been identified in about 30% of all human cancers,
indicating tha...
... BAB64536) and AtFer1 and AtFer4
from A. thaliana. While it is clear that AtFer1 is a poor
candidate for a mitochondrial localization, for the other
proteins significant scores were found. In particular,
PSORT
and
IPSORT
programs ... mitochondria
(Table 3).
The data presented in this paper strongly indicate a
mitochondrial localization for ferritins in P. sativum and
A...
... a metal-to-ligand
charge transfer band at approximately 520 nm [27].
Substrate binding adjacent to the Fe(II) is proposed to
weaken binding of the remaining coordinated water,
thus enabling the ... MJ, Padmakumar R & Hausinger RP (1999)
Stopped-flow kinetic analysis of Escherichia coli tau-
rine ⁄ alpha-ketoglutarate dioxygenase: interactions with
alpha-ketoglutarate, taurine...
... muta-
genesis data therefore provide additional critical infor-
mation over that obtained from X-ray data alone. The
knowledge gained from these mutagenesis data will be
valuable in directing the ... and ACE2. The zinc prote-
ase domain of both tACE and ACE2 is divided into two subdomains
[20]. Subdomain I contains the zinc ion and the N-terminus. The
C-terminus is found...
... (peptide backbone and side
chains) exposed on denaturation. For various osmo-
lytes, Bolen & Baskakov [3] have shown that: (a) the
main driving force for the folding is the unfavourable
interaction ... 1–12.
24 Singh LR, Dar TA, Haque I, Anjum F, Moosavi-
Movahedi AA & Ahmad F (2007) Testing the paradigm
that the denaturing effect of urea on protein stability is
offset b...
... Italy
Therapeutic strategies aimed at reducing brain dam-
age after ischemic stroke have been a major focus of
academic and industrial research for the past
30 years. Two primary therapeutic approaches ... pro -in am-
matory mediators is probably a result of the fact that
in ammatory transcription factors such as nuclear
factor-kappaB, activator protein-1 and nuclear factor
of ac...