Báo cáo khoa học: Copper-containing nitrite reductase fromPseudomonas chlororaphis DSM 50135 Evidence for modulation of the rate of intramolecular electron transfer through nitrite binding to the type 2 copper center pot
... Copper- containing nitrite reductase from
Pseudomonas chlororaphis
DSM 50135
Evidence for modulation of the rate of intramolecular electron transfer through nitrite
binding to the type 2 copper ... Biochem. 27 1) 23 67
potential. However, addition of nitrite to the reduced
form of the enzyme causes more complete reoxidation of...
... 100
Ca
2+
0. 52 ± 0.01 26
Cr
2+ a
0.38 ± 0.01 19
Mn
2+
2. 23 ± 0.01 1 12
Co
2+
2. 14 ± 0.04 107
Ni
2+
2. 42 ± 0.09 122
Cu
2+
0.80 ± 0.05 40
– 0.54 ± 0. 02 27
a
Cr
2+
added might be oxidized to Cr
3+
by ... by the reactivase in the presence of
ATP and Mg
2+
at a molar ratio of the reactivase to the
enzyme of 0.1 is shown in Fig. 3. From the initial ra...
... to occur at higher concentra-
tions of the metal ion [45]. The addition of E64, a syn-
thetic inhibitor of calpain, did not modify the intensity
of the signal observed in the presence of Ca
2+
alone.
The ... direct evidence of the nature of the
ligand responsible for the observed conformational
transition. Thus, to discriminate between the effect du...
... attach to
NPs rather than VPs and moreover attach most often to the
lowest, nearest NP is important for the semantic interpreta-
tion of sentences because of the combinatorial explosion of
possible ... affect the form of the dis-
course. Examples of dimensions arc: interaction, the extent
to which user and adviser can quickly interact, respond to
each oth...
... Journal 27 2 (20 05) 4983–4995 ª 20 05 FEBS 4983
sequence found at the C-terminus of PEA is essential
for the cytotoxicity of the endocytosed toxin, allowing
PEA to reach its site of action [2, 5]. ... Journal 27 2 (20 05) 4983–4995 ª 20 05 FEBS 4985
of intoxication. Neither the human leukemic cell line
HSB -2 nor the Burkitt lymphoma Ramo cells showed
any potentiat...
... FEBS 20 03
protection of either of the activities was observed (Fig. 4C).
SDS/PAGE of the digest demonstrated the disappearance
of the parent molecule, with generation of the two fragments
of 45 ... or
removal of the equatorial OH on C2 is known to abolish all
substrate interactions. 2- Deoxy-
D
(+)-glucose and 2- deoxy-
D
(+)-galactose, which act as inhibitors...
... electrostatic potentials of
the surface of the CPR FMN domain and of a model
of the FMN domain of human MSR.
Fig. S3. Electrostatic potentials of the surface of the
hMS AD.
Table S1. Sequences of oligonucleotides ... (AqCbl) to
form cob(II)alamin. Reduction of the Co centre
promotes the dissociation of the lower dimethylbenz-
imidazole base of the cof...
... beyond 10 h of
growth, as compared to the wild -type. The growth
rates of both the wild -type and the mutant were com-
promised at pH 5.0 (not shown).
The addition of urea (10 mm) to the medium ... growth for
both the wild -type and the mutant (data not shown).
There was also a marked increase in the pH of the
medium after 48 h of bacterial growth for b...
... of an Arabidopsis DExD ⁄ H box RNA helicase R R. Xu et al.
23 02 FEBS Journal 27 8 (20 11) 22 96 23 06 ª 20 11 The Authors Journal compilation ª 20 11 FEBS
we applied this technique to clarify genotype ... plates. Seeds were stratified at
4 °C for 2 days, and then transferred to 22 °C for 2 weeks.
Col-0 was used as the wild type, and was the genetic back-
ground fo...