Báo cáo khoa học: Cloning and characterization of the genes encoding toxic lectins in mistletoe (Viscum album L) pot

... (Met153 of the ML1p and ML2p B-chains and Met156 of the ML3p B-chain corresponding to the ricinB-chain Leu152; and Met234 of the ML1p and ML2pB-chains corresponding to the ricin B-chain Leu233).Estimation ... RIPs (such as ricin and abrin) [2], the mlp genes do not contain introns and they encode the toxic lectins in the form of a single chain precursor (Fig. 1). The ML1p precursor, like the rML-precusor ... Asn255 and Gln256 of the ricin B-chain in the second sugar binding site [28]. The exceptions are the replacement of Trp38 ﬁ Ser in the ML2p B-chain and Tyr252 ﬁ Phe in the ML3p B-chain (Fig. 2).The...

Tài liệu Báo cáo khoa học: Cloning and characterization of CBL-CIPK signalling components from a legume (Pisum sativum) ppt

... calcium binding domains (EF1–4) and calcineurin A binding domain are shown in the box. The dot in the EF1 box repre-sents the modiﬁed amino acids alanine (A) as compared to the oxygen containing-calcium ... unshaded. The amino acids marked by red, blue, green and pink lines indicates the putative casein kinase II, protein kinase C, the cAMP- and cGMP-dependent protein kinase and putative tyrosinekinase ... deletion in the autoinhibitory(NAF) motif failed to interact with PsCBL thus con-ﬁrming the authenticity of these proteins and emphasi-zing the importance NAF in the interaction betweenthem (data...

Tài liệu Báo cáo khóa học: Cloning and characterization of two distinct isoforms of rainbow trout heat shock factor 1 ppt

... 271) 705 encoding the DNA-binding domain of HSF, whereas cloneC2 contained an insert of 2771 nucleotides including introns and an ORF encoding 513 amino acids.By using 5¢ -and3 ¢-RACE, the full-length ... (a3 and b3) and two heterotrimers (a2b1 and a1b2). The existence of the four types of trimer may be reﬂected in the broadband migrating at  200 kDa in Fig. 7. On the other hand,a band ... cycle of 15 min at 95 °C,ampliﬁcation cycles of 0.5 min at 94 °C, 0.5 min at 50 °C and 0.5 min at 72 °C, and one termination cycle of 1 min at72 °C, with 35 cycles in total for HSF1a and HSF1b and...

Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx

... vectorfollowing the manufacturer’s instruction (Gateway, Invi-trogen). The correct insertion of the Rv1399c ORF wasconﬁrmed by DNA sequencing. In our hands, protein expression using the commerciallyavailable ... Lac O (the DNA binding site of LacI). The resulting construct constitutively expresses Lac I,which inhibits the T7 RNA polymerase in b inding to itsspeciﬁc DNA binding site on Lac O.Mutagenesis. ... that of acetylcholinesterase (30 mM). The kinetic behavior of Rv1399c using short-chain soluble vinylesters and triacylglycerols along with the lack of d etectableactivity using insoluble vinyl...

Báo cáo khoa học: Cloning and expression of the ﬁrst nonmammalian interleukin-11 gene in rainbow trout Oncorhynchus mykiss pdf

... Li R, Hartley L & Robb L (2001) Cloning of rat inter-leukin 11 and interleukin 11 receptor alpha chain and analysis of their expression in rat uterus in the peri-implantation period. Reproduction ... (NQT) are in bold and underlined. The four potential poly(A) signals (AATAAA) in the 3¢-UTR and the TATAbox in the 5¢-ﬂanking region are boxed.T. Wang et al. Rainbow trout interleukin-11FEBS ... 1139with 21 amino acids in mammals. There are cysteineresidues (one in trout and four in fugu) and potentialN-glycosylation sites (one in trout and two in fugu)present in mature peptides of the ﬁsh...

Báo cáo khoa học: Expression and characterization of the bioﬁlm-related and carnosine-hydrolyzing aminoacylhistidine dipeptidase from Vibrio alginolyticus pot

... family and contains a di-zinc binding domain and a smalldomain that is inserted in the middle of the metal-binding domain and mediates catalysis. The di-zincFig. 6. Metal effects on the enzymatic ... downstream from His80 in the vicinity of the zinc center and is assumed toclamp the imidazolium ring of His80. Glu149 is in the immediate vicinity of the Glu150 of the zinc center and is assumed to ... residues in the stability of di-zinc binding and suggest thatthese residues are essential for the enzymatic activity of the PepD.To investigate whether site-directed mutagenesis of the amino acid...

Báo cáo Y học: Cloning and characterization of the mammalian-speciﬁc nicolin 1 gene (NICN1) encoding a nuclear 24 kDa protein doc

... We obtained a human and amurine NICN1 cDNA clone from the IMAGE collection and determined the complete sequences of these cDNAs. The comparative analysis of the human, canine, and murineNICN1 ... embryos. These experimentsshowed auniformstaining of the mouse embryos (data not shown) indicating the presence of Nicn1 transcripts in all tissues of the developing embryosat the investigateddevelopmental ... genomic clones of the NICN1gene The isolation of the canine genomic BAC contig containing the canine NICN1 gene has been described previously [3].BLAST searches against the human and murine ESTdatabases...

Báo cáo khóa học: Isolation and characterization of the Xenopus HIVEP gene family ppt

... contribution of these elements to ZAS-N Znf binding, mutations werecreated in either the GAGA box or the trinucleotide CCCsequences in a 27-mer duplex spanning the protectedregion. The binding of the ... by the generation of deletion mutants containing discretefunctional domains of XHIVEP1. Thus, the cloning and characterization of the XHIVEP interacting factors wouldbe of interest and should also ... over the proteins,including several serine-rich stretches [9]. The 8578 bp cDNA sequence of XHIVEP1 contains242 bp of the 5¢-UTR, an open reading frame of 7734 bp and 602 bp of the 3¢-UTR. The...

Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx

... Taken together with the inability of P III and P IV to inhibit hemagglutination in vitro in the presence of this sugar, these data suggest that the bind-ing and uptake of these two isoforms does ... pulchellus seeds contain at least seven closely related and highly toxic type 2 ribosome-inactivating pulchellins, each consisting of a toxic A-chainlinked to a sugar binding B-chain. In the present study, ... discussed.ResultsNomenclature of the toxic pulchellin lectins The abbreviation P is followed by the Roman numer-als I, II, III and IV and refers to each pulchellinisoform (P I, P II, P II and P IV). The A-chain of P...

Tài liệu Báo cáo khoa học: Production and characterization of a secreted, C-terminally processed tyrosinase from the ﬁlamentous fungus Trichoderma reesei ppt

... coincide with the N-terminus of the intracellular enzyme (Fig. 1). The segments in the N-terminal portion around the copper ligandamino acids of the active site are well conservedbetween the ... J-C, Asther1 M & Lomascolo A (2006) Cloning and characterization of a tyrosinase gene from the white-rot fungus Pycnoporus sanguineus, and overproduction of the recombinant protein in Aspergillusniger. ... determining enzyme activity on 15 mml-dopa in the presence of the inhibitors. The concentration of the inhibitor was 0.1, 1, 10 or 100 mm, depending on the in- hibition efﬁciency. At least two inhibitor...

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