Báo cáo khoa học: Covalent activation of heart AMP-activated protein kinase in response to physiological concentrations of long-chain fatty acids docx

... syn-thase, phosphofructokinase 2, ceramide synthesis, glucoseuptake, apoptosis, insulin receptor substrate 1, mammaliantarget of rapamycin kinase (mTOR), mitogen-activated protein kinase kinase 3, ... oxidation.Keywords: AMP-activated protein kinase; fatty acids; heart; insulin; protein phosphorylation.The AMP-activated protein kinase (AMPK) is a heterotri-meric enzyme complex with a key role in the ... blockedinactivation of the AMPK by insulin (Fig. 7), suggesting adominance of the fatty acid-driven pathway for activation of AMPK over at least some aspects of insulin signalling.This dominance of...

Báo cáo khoa học: Relationship between functional activity and protein stability in the presence of all classes of stabilizing osmolytes ppt

... refolding of misfoldedproteins [5–8] and remove protein aggregation [9–12].Mechanisms of protein osmolyte interactions, theeffect of osmolytes on protein stability, and how osmo-lytes correct protein ... leading to the preferential hydration of the protein domain is the driving force of protein stabiliza-tion or folding [3,4]. Furthermore, the effect of osmolytes on the functional activity of ... andsorbitol). Counteracting osmolytes consist of themethylamine class of osmolytes, which are believed to have the special ability to protect intracellularproteins against the inactivation...

Báo cáo khoa học: Transthyretin and familial amyloidotic polyneuropathy Recent progress in understanding the molecular mechanism of neurodegeneration pdf

... on the interaction betweenprealbumin, retinol-binding protein, and vitamin A.J Biol Chem 246, 44–49.71 Bellovino D, Morimoto T, Tosetti F & Gaetani S(1996) Retinol binding protein and ... important role in a variety of physiological eventsand regulates nuclear factor k-B (NF-kB), mitogenactivated protein kinase (MAPK), and Jun – N-ter-minal kinase (JUNK) signaling [145], all of which ... due to an allostericeffect resulting from the occupancy of the ﬁrst bindingsite [68]. TTR is also involved in the transportation of retinol by forming a complex with the smaller retinol-binding...

Báo cáo khoa học: Tec family kinases Itk and Rlk⁄ Txk in T lymphocytes: cross-regulation of cytokine production and T-cell fates pot

... differentiationand function of distinct cytokine-producing, effector T-cell populations.AbbreviationsBTK, Bruton’s tyrosine kinase; IFN, interferon; IL, interleukin; iNKT, invariant natural killer ... Tec kinases contribute to human diseases involving distinct types of T-cell activation and cytokine production. In this minireview,we cover the roles of Itk and Rlk ⁄ Txk in T-cell recep-tor ... of cytokine-producing populations that also play keyroles in shaping immune responses, implicating Tec kinase signaling in multiple levels of regulation of cytokine production.Itk and the regulation of cytokine-producing...

Báo cáo khoa học: NARG2 encodes a novel nuclear protein with (S/T)PXX motifs that is expressed during development docx

... DNA-bindingdomain that is presen t in many transcription factorsincluding Kruppel, Hunchback and Antennapedia [14], aswell as other DNA-binding proteins [28,29]. There are 11repeatsof(S/T)PXXinmNARG2(seveninexon10),andsix ... stretch of basic amino acids near the C-terminus of the protein (KKRKKIRR, amino acids 764–771 of the mouse sequenceÓ FEBS 2004 NARG2, a novel nuclear (S/T)PXX protein (Eur. J. Biochem. 271) 4631 in ... the NARG2amino acid sequence, starting at the N-terminus of the protein. Thestretch of amino acids derived from exon 10 is indicated between twobroken vertical lines. Th e rate of synonymous...

Báo cáo khoa học: Aldo-keto reductase from Helicobacter pylori – role in adaptation to growth at acid pH doc

... quantitation of microgram quantities of protein utilizing the principle of protein- dye binding. AnalBiochem 72, 248–254.39 Sambrook J & Russell DW (2001) Molecular Cloning:A Laboratory Manual. ... been identiﬁed using a random insertionalmutagenesis technique [3]. Several of these wereproteins of unknown function, including an aldo-ketoreductase (AKR). The role of the AKR in acid adapta-tion ... and ketones to the corre-sponding alcohol product, and thus serve to removepotentially cytotoxic and mutagenic aldehydes fromcells [14]. In view of the potential aldehyde detoxifying role of AKR...

Tài liệu Báo cáo khoa học: Synergistic activation of signalling to extracellular signal-regulated kinases 1 and 2 by epidermal growth factor and 4b-phorbol 12-myristate 13-acetate pptx

... actor (EGF),which bind to dedicated receptors. Upon EGF binding, itsreceptor (EGFR) dimerizes, leading to autophosphoryla-tion of tyrosine residues on the cytoplasmic domain of thereceptor, ... signal-regulated kinase; ERK-PP,doubly phosphorylated ERK; Grb2, growth factor receptor binding protein 2; IP3, inositol triphosphate; MAPK, mitogen-activated pro-tein kinase; MEK, MAPK/ERK kinase; MKP, ... ltrasensitivitycan bring about oscillations in the mitogen-activated protein kinase cascades. Eur. J. Biochem. 267, 1583–1588.23. Livneh, E. & Fishman, D.D . ( 1997) Linking protein kinase C to cell-cycle...

Tài liệu Báo cáo khoa học: Covalent binding to glutathione of the DNA-alkylating antitumor agent, S23906-1 doc

... reduction of the cytotoxicity of S23906-1 is attributed to the reducedformation of lethal drug–DNA covalent complexes in cells.Treatment of the cells with buthionine sulfoximine, aninhibitor of GSH ... reduction of cytotoxicity by a factor of about six. In this case, the IC50value of 4.2 lMdetermined with mechlorethamine aloneincreased to 24.8 lM in the presence of mechlorethamineand 1 ... failed to reactcovalently with S23906-1. Gel shift assays and ﬂuorescencemeasurements indicated that the binding of S23906-1 to DNA and to GSH are mutually exclusive. Binding of S23906-1 to an...

Báo cáo khoa học: Proteolytic activation and function of the cytokine Spatzle in the innate immune response of a lepidopteran insect, Manduca sexta ppt

... FEBSIntroductionA prominent feature of the innate immune systems of insects is the activation of serine proteinase cascadepathways in hemolymph, which function to activateplasma proteins that ... function of the cytokine Spa¨tzle-1is conserved in the immune system of a lepidopteraninsect, suggesting that a cytokine-activated Toll path-way is an ancient feature of innate immunity in insects.Although ... Thismechanism leads to activation of phenoloxidase, whichoxidizes catechols, leading to the formation of toxicquinones and melanin [1,2], and to the activation of cytokines that stimulate hemocyte...

Báo cáo khoa học: Covalent and three-dimensional structure of the cyclodextrinase from Flavobacterium sp. no. 92 pdf

... domainB (indicated in Fig. 2). This inserted domain participates in substrate binding and is rather variable. It is considered to play a role in determining the enzyme speciﬁcity [41]. Theend of ... Theremoval of Ca-I was shown to promote proteolysis [43,44].Ca-I is co-ordinated by the side chains of Asp280 andSer222 at the beginning and end of domain B as well as bythe main-chain oxygens of ... domain to make an intimate contact across thestrong dimer interface with domains A and C of the othersubunit. In contrast, the TVA-II group dimer attaches the Bdomain to an N-terminal domain of...

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