... Tyr42 (in RNase T1) or an arginine (in RNase Sa, barnase, and binase) has an important role in closing the guanine-binding site [10,15–17]. How-ever, although the interactions between guanine andthe ... molecules, guanosine formed the high-est number of < /b> hydrogen bonds of < /b> all the bases, up tofive, and had the best fit into the base-binding site. In addition, guanine underwent a stacking interactionwith ... observed both horizontalshifts and rotation of < /b> the base with respect to theplane of < /b> the guanine, by up to 40°, disrupting thePhe39–base stacking interaction.To find possible binding subsites of...