... fibroblasts and human carci-
noma tissues of the uterine cervix, breast and urinary bladder is
shown. NANOGP8 was expressed in all of the human cancer tis-
sues that were tested. NANOGP8 was not expressed ... resulted in only one amino acid change in
the predicted protein sequence from (Gln, 757CAG)
in Nanog and (His, 757CAC) in NANOGP8. Since
NANOGP8 has an intact open-...
... Isolation, sequencing
and mutational analysis of a gene cluster involved in
nitrite reduction in Paracoccus denitrificans. Antonie
Leeuwenhoek 66, 111–127.
13 Kawasaki S, Arai H, Kodama T & ... is the His residue that in
NirS is the proximal axial ligand to the d
1
heme.
Replacement of an equivalent His, His41, in NirF by
Ala abolished binding of the heme to the protein.
Know...
... (AAC71532) and with the putative triacylglycerol lipase
AAB96044 from Mycoplasma pneumoniae (Mp). Identical amino
acids are indicated by an asterisk and similar amino acids are indi-
cated by a colon and ... Woolford CA, Noble JA, Garman JD, Tam MF, Innis
MA & Jones EW (1993) Phenotypic analysis of protein-
ase A mutants. Implications for autoactivation and the
maturation pathway of t...
... disRAS1fwd
5¢-TTCACGATTGAACAGGTAAACAAAATTTTCC
CTTTTTAGAACGACATGCAGCTGAAGCTTCGTA
CGC-3¢ and disRAS1rev CAAAACCATGTCATAT
CAAGAGAGCAGGATCATTTTCAACAAATTATGC
ATAGGCCACTAGGGATCTG-3¢. YEp351-SUT2 was
constructed to contain SUT2 as the only open reading
frame present in the plasmid in ... 5¢-GACTGTCGATGATTATGGTTGCC
CGCTGGCTTCCAAACCCTTATCGATACCGTCGA
CCC-3¢ and SUT-GFPrev 5¢-AACAATTTCACACACA
GGAAAC...
... Slc1 2a2 mRNA were designed as
follows; Slc1 2a2 antisense, 5¢-ATCTTCACAAGAAAAAT
CACCTGGTACCAAGGATGT; Slc1 2a2 sense, 5¢-ACAT
CCTTGGTACCAGGTGATTTTTCTTGTGAAGAT.
All experimental protocols described in ... target genes. The data were deposited in GEO
database GSE2043 (a complete list of these genes
appears in Table S1 and a partial list is shown in
Table 1). Because only a single...
... transvacuolar strands and maintain overall
cellular architecture. As mentioned above, CRP1 may
participate in the formation and ⁄ or maintenance of
long actin cables [12]. Consistent with this ... proteins and interacts with
skeletal a- actin in the cytoplasm. However, little is
known about the mechanism whereby hhLIM interacts
with skeletal a- actin and regulates the organization
and...
... in
amylin aggregation, inhibiting amylin aggregation in
healthy individuals, but promoting aggregation during
T2D pathogenesis. In summary, amylin–insulin inter-
actions are most likely to play a complex ... concentrations of
insulin. Taking amylin alone as a reference, after incu-
bation for 6 h (Fig. 3A) , approximately half of
the fibril formation was inhibited when amylin was
inc...
... Miyamoto K, Tanaka K, Kawai M, Tainaka
K, Imada C, Okami Y & Inamori Y (1993) Cloning
and sequence of an alkaline serine protease-encoding
gene from the marine bacterium Alteromonas sp. strain
O-7. ... standard.
Standard enzyme assay
SPRK activity was routinely measured using Suc-Ala-Ala-
Pro-Phe-pNA (Sigma Aldrich) as substrate. The peptidase
assay was carried out in a total volume o...
... proctolin (Arg-Tyr-Leu-Pro-Thr) have been
compared to those of enkephalins. Indeed, a dipeptidyl
aminopeptidase activity appears as one major proctolin-
degrading peptidase, liberating the N-terminal ... degradation studies using proctolin (40 l
M
)
as the substrate. SDS/PAGE analysis of fractions contain-
ing partially purified DPP activitiy revealed two major
bands in the range of 82 and...