Báo cáo khoa học: Stage specific expression of poly(malic acid)-affiliated genes in the life cycle of Physarum polycephalum Spherulin 3b and polymalatase potx
Báo cáo khoa học: Stage specific expression of poly(malic acid)-affiliated genes in the life cycle of Physarum polycephalum Spherulin 3b and polymalatase potx
... 1055 Stage specific expression of poly(malic acid)-affiliated genes in the life cycle of Physarum polycephalum Spherulin 3b and polymalatase Nadthanan Pinchai, Bong-Seop Lee and Eggehard HollerInstitut ... Among them, spheru-Fig. 2. Structural alignment of amino acid sequence by motifs, of spherulin 3b with spherulin 3a and other members of the bc-crystallin fam-ily: (1) spherulin 3b from P. polycephalum; ... insight,this investigation was aimed at identifying plasmo-dium -specific genes, which are involved in the synthesis of PMLA and ⁄ or its regulation, and to clarify whether the stage- specific expression of...
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Báo cáo khoa học: Stage-specific expression of Caenorhabditis elegans ribonuclease H1 enzymes with different substrate specificities and bivalent cation requirements ppt
... that of Ce-RNH1a in the presence of Mg2+ and about one-fortieth in the presence of Mn2+. The difference in specific activities betweenCe-RNH1a and Ce-RNH1b suggests that the existence of the ... also determined the spe-cific activities of Ce-RNH1a and Ce-RNH1b in the presence of Mg2+or Mn2+ions, as shown in Table 2.Although the specific activity of Ce-RNH1a in the presence of Mg2+was ... Hilden,Germany). The nucleotide sequences of each insert DNAwere determined and confirmed to be identical with those in the database. Expression and purification of recombinantproteins The ORFs of each...
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Báo cáo khoa học: Stage-specific activation of MIG-17⁄ADAMTS controls cell migration in Caenorhabditis elegans docx
... against polyhistidine revealed the same-sizedbands, suggesting that the C-terminal histidine tagremained in both the proform and activated form in the recombinant enzyme after its secretion into ... to study the function of ADAMTSs during organogenesis in vivo. In this study, we isolated recombinant MIG-17 and determined the amino acid sequence of the N-ter-minal neo-epitope of the activated ... basementmembrane of the muscle and the gonad (Fig. 4E).These results indicate that activation of MIG-17occurred in the basement membranes of the muscle,intestine and gonad in the L3 larvae.We...
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Tài liệu Báo cáo khoa học: Pyrimidine-specific ribonucleoside hydrolase from the archaeon Sulfolobus solfataricus – biochemical characterization and homology modeling doc
... cytosine were 12.2 and 6.2 min, 10.5 and 4.7 min,15.2 and 6.1 min, 6.8 and 4.2 min and 6.6 and 3.9 minrespectively. The amount of purine or pyrimidine baseformed is determined by integrating the ... mixture of 95% methanol and 0.1% trifluoroacetic acid in H2O. The retention times of adenosine and adenine, inosine and hyp-oxantine, guanosine and guanine, uridine and uracil, cyti-dine and cytosine ... [48].Docking of ribose and Ca2+into the binding site of SsCU-NH was performed using, as a starting reference, the position of the sugar and of the metal in the crystallo-graphic template 1YOE. The...
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Tài liệu Báo cáo khóa học: Non-specific depolymerization of chitosan by pronase and characterization of the resultant products pptx
... beadcharacteristics, in which increases in molecular mass and DA resulted in efficient loading and minimization of the release of entrapped lipase [30]. The observed increase in specific activity with increasing ... 3600–3000 cm)1 in the spectra of bothchitosan and LMWC indicated the absence of free -OHgroups and the involvement of both -OH… 3and -CH2OH…6 in intra- and intermolecular hydrogen bonding(Fig. ... largerd-spacing as a result of increase in the unit cell dimensionthat results in the incorporation of fewer water molecules.Decrease in the intensity of 020 reflections in LMWC wasalso indicative of...
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Báo cáo khoa học: Calcite-specific coupling protein in barnacle underwater cement docx
... physiological conditions, and confirmed to retain almost the same structure as that of the native protein. Both the protein from the adhesive layer of the barnacle and the recombinant protein were character-ized. ... characterize the nature of the protein in the primary cement. Thereaf-ter, we expressed the recombinant form of the protein in bacteria in a soluble form under physiological con-ditions, and confirmed ... 8). The difference between the B. albicostatus protein and Mrcp-20k in their amino acid lengths depended on the difference in the number of repeats. The similar Cysspacing, the existence of Pro...
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Báo cáo khoa học: Ligand-specific dose–response of heterologously expressed olfactory receptors potx
... excitation) in the absence of calcium, and Fsis the 510 nm emission signal (380 nmexcitation) with a saturating concentration of calcium. Z1 and Z2 are the intrinsic fluorescence intensities of the ... in Krautwurst’s study [3], involving expression in HEK293cells of a chimeric receptor including the N-terminus of rhodopsin and full-length I7 sequence and Ga15,16,octanalinduced a response at ... modulation of the odorant–receptorinteractions depending on odorant chain length, within the putative ligand-binding site determined by transmembranedomains IV–VII, which will be further investigated...
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Báo cáo khoa học: Site-specific phosphorylation of MCM4 during the cell cycle in mammalian cells pot
... re-replication of DNA in a single cell cycle. Inactivation of CDK1 leadsto re-replication of DNA in eukaryotic cells includinghuman cells [7]. Targets of the kinase in the regulation of DNA replication include ... dur-ing the HeLa cell cycle in a site -specific manner.Cyclin-dependent protein kinase is involved in the phosphorylation of MCM4To determine which kinase is involved in the phos-phorylation of MCM4 ... presence of geminin, which inhibits the ability of CDT1 to load MCM proteins onto chromatin, mayhelp prevent re-replication of DNA in the G2 and Mphase. During interphase, chromatin-bound...
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Báo cáo khoa học: Hepatocyte-specific interplay of transcription factors at the far-upstream enhancer of the carbamoylphosphate synthetase gene upon glucocorticoid induction doc
... did not result in any changes in the pattern of bands for either of the two cell types, showing that binding of C ⁄ EBP and FoxA to the CPS GRU in FTO-2B cells is independ-ent of these hormonal ... the lowerstrand. FoxA binding resulted in protection of the gua-nines at positions 347 and 351 on the upper strand, and protection at position 352 on the lower strand.These footprints in the ... sta-bilization of GR binding to the GRU, and therebystabilization of the interaction of the coregulatorsinteracting with the nucleoprotein complex formed at the GRU. This raises the possibility that the...
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Báo cáo khoa học: Site-specific casein kinase 1e-dependent phosphorylation of Dishevelled modulates b-catenin signaling ppt
... key substrates of casein kinase I (CKI). However, the specific sites phosphorylated in vivo by CKI and their relative import-ance in the physiologic regulation of these proteins in the canonical ... phosphor-ylated in vivo.Casein kinase I epsilon (CKIe) is a positive regulator of the Wnt signaling pathway downstream of Wnt and upstream of Dvl, axin and b-catenin. CKIe and Dvl physically interact in ... wehypothesized that mutating serines 139 and 142 to ala-nines would diminish the intensity of signaling in HEK293 cells. b-Catenin ⁄ Lef-1-dependent transcriptionwas assessed using the luciferase...
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