... FEBS
Promoters of type I interferon genes from Atlantic salmon
contain two main regulatory regions
Veronica Bergan, Silje Steinsvik, Hao Xu, Øyvind Kileng and Børre Robertsen
Department of Marine Biotechnology, ... transcription start site of their
promoters [19]. The IFN-b promoter contains four
positive regulatory domains (PRDs), which bind IRFs:
mainly I...
... classi-
fied into two types, according to their ability to cleave
one (type I) or both (type II) strands of a DNA double
helix [1,2]. Type II topoisomerases can be divided into
two subclasses: type ... fusion of full-length and partial OsTOP6B with GAL4 DNA-binding domain, respectively. The
interaction of BD-53 (fusion of p53 with GAL4 DNA-binding domain) with AD-T (fusion...
... species.
Discussion
The distribution of ADHs in the brain is of particular
interest because of their implications in the metabolism of
ethanol and retinoic acid. In vitro and in vivo data indicate
that ... protein level,
making it difficult to decide which of the ADH genes or
proteins is expressed in a certain tissue. In previous studies
employing Northern blot analysis, tissue dis...
... N-(5-phospho-d-ribonoyl)-methylamine
Fig. 2. Inhibition of TcRpiB Rpi activity by All6P. Activity in the
isomerization of R5P was tested using a direct spectrophotometric
assay, as described within the ... (1996) Ribose catabo-
lism of Escherichia coli: characterization of the rpiB
gene encoding ribose phosphate isomerase B and of the
rpiR gene, which is involved in regulation of...
... pathway elicited by CNR1
comprises inhibition of adenylylcyclase via G
i
pro -
tein and consequent activation ⁄ inhibition of Ca
2+
and K
+
channels; activation of mitogen-activated
protein kinase ... are in black boxes;
identical amino acid residues are in light
gray boxes; similar amino acid residues are
in medium gray boxes; different amino acid
residues are in white boxes. Similar...
... areas containing dimers;
the larger lattice of origin is indicated in Fig. 1
(area 4). LH2’s were found to be aligned, forming
rows along the long axis of the dimers within a period-
ical lattice. ... originate from LH2 dimers.
More evidence for the dimeric origin of zig-zag
lattices can be obtained from high-resolution AFM
images of dimers and zig-zag LH2’s, as shown in
Fig. 5. I...
... acid biosynthesis [22]. The functional
diversity of simple homodimeric type III PKSs is
derived from small differences in the active site that
influence the substrate specificities, the number of ... rapid and sensitive method for
the quantitation of microgram quantities of protein
utilizing the principle of protein–dye binding. Anal
Biochem 72, 248–254.
Octaketide synthase from...
... MALDI-TOF mass spectrometry.
Two microlitres of purified ASP3c were mixed with 2 lLof
matrix solution (saturated solution of sinapinic acid in 30%
v/v acetonitrile, 0.2% v/v trifluoroacetic acid). ... CSPs
indicates that disulfide bond pairing Cys (I) –Cys(II) and
Cys(III)–Cys(IV) is probably shared by all members of this
insect protein family. Because the cysteine residues of the
reco...
... activity
is specific activity multiplied by total protein in milligrams; after Triton X-100 extraction, there is a > 3-fold activation of activity, which is
reflected by an increase in total activity. ... detected binding of antibody against
POP in internal membranes in immunohistochemistry
studies in rat brain [11]. However, no clear identifica-
tion of the protein responsible for this...
... precise size
of the Ca
2+
deficit introduced.
[1 ⁄ 3-OH]Inositol pentakisphosphate accompanies
InsP
6
in the purified deposits and in intact
parasite tissue
During our initial detection of InsP
6
in ... corresponding to InsP
6
and to two different inositol monophosphate species
(Fig. 4E).
Discussion
Formation of solids is an important aspect of InsP
6
biology, the understanding of w...