Báo cáo khoa học: DNA-binding characteristics of the regulator SenR in response to phosphorylation by the sensor histidine autokinase SenS from Streptomyces reticuli doc

... 2007 The Authors Journal compilation ª 2007 FEBS 3913 DNA-binding characteristics of the regulator SenR in response to phosphorylation by the sensor histidine autokinase SenS from Streptomyces reticuli Gabriele ... characterization of the DNA-binding properties in response to phosphoryla-tion by the sensorkinase SenS. ResultsCloning of wild-type and mutant senS cand senR genes and puriﬁcation of fusion proteinsAs ... assumptionthat phosphorylation of SenR by SenS P alters its DNA-binding characteristics. Discussion The designed cloning procedures allowed us to obtain the cytoplasmic domain of SenS carrying a Strep-tag (SenS c)...

Tài liệu Báo cáo khoa học: Molecular defect of isovaleryl-CoA dehydrogenase in the skunk mutant of silkworm, Bombyx mori ppt

... neigh-bouring monomer in the tetramer. By contrast, the side chain of the mutated residue (Val376) pointstoward the inside of the helix–loop–helix structure.Consequently, the side-chain atoms of Val376 ... Determination of full-length cDNAsequences using the RACE method in both strainsrevealed the presence of a single point mutation in 1337 nucleotides of BmIVD. In the sku mutant, the 1127th guanine ... [7]. The model of wild-typeBmIVD indicated that all the side chains in the loopconnecting helices I and J are exposed on the surface of the monomer, pointing toward FAD or its neigh-bouring...

Tài liệu Báo cáo khoa học: Molecular aspects of rheumatoid arthritis: chemokines in the joints of patients pdf

... in am-matory disease that occurs in about 1% of the popula-tion. The in ammatory process is characterized by in ltration of in ammatory cells into the joints, leading to the proliferation of ... chemokines stimu-lated mainly by in ammatory cytokines, including IL-1b, TNF-a andIFN-c. (B) Chemokines expressed in the joint recruit leukocytes into the joints. In addition to functioning in ... primarily affects the small joints of the hands and feet. The in ammatory process is characterized by in ltration of in ammatory cellsinto the joints, leading to proliferation of synoviocytes...

Tài liệu Báo cáo khoa học: Electrostatic role of aromatic ring stacking in the pH-sensitive modulation of a chymotrypsin-type serine protease, Achromobacter protease I pdf

... to the pKa of His210, is mainly determined by the type of side-chain atresidue 169. In the Trp169 mutants, His210 deprotonates ata lower pH compared to that for wild-type API, due to the increased ... size of the side-chain at residue 169 and the pK2showed a clear linear relationship (Fig. 3B). The pK2increased as the size of the residue at position 169 increased. The effect of the size of ... temperature, the interchange rate of the proton between His57 Nd1-Asp113 Oc2 may be too fast to monitor as the split signals,while at low temperature, that of the interchange rate is tooslow to monitor...

Báo cáo khoa học: "AN APPLICATION OF AUTOfIATED LANGUAGE UNDERSTANDI;IG TECHNIQUES TO THE GENERATION OF DATA BASE ELEMENTS" potx

... attempt to find fillers for the descriptor slots of the temp]ate in question, and a third argument which re- presents the output of the procedure, i.e., the in- stantiated slots. The body of the ... flects the information user's conceptualization of the domain, i.e., his view of what that class of entities involves. In the domains under investigation there are templates for classes of ... consist of descriptions of events involving aircraft activities and launchings of missiles and satel]ites. The source data are contained in the text portions of military messages typical of these...

Báo cáo khoa học: Combined use of selective inhibitors and ﬂuorogenic substrates to study the speciﬁcity of somatic wild-type angiotensin-converting enzyme docx

... the N-domainand C-domain (indicated by the arrow in Fig. 1A).Thus, information on the selectivity of both the sub-strate and the inhibitor for the N-domain andC-domain can be determined from ... the binding of RXP407 to the N-domain led to 80% inhibition of its activity(arrow in Fig. 1A, closed circle), whereas in the ratenzyme binding of RXP407 to the N-domain only gave20% inhibition ... C-domain varies according to the ACE species. For rat and human enzymes, estimation of IC50values for the binding of the inhibitor to the N-domain and C-domain can be deduced from the inhibition...

Báo cáo khoa học: Membrane binding of SRP pathway components in the halophilic archaea Haloferax volcanii docx

... presently of the nature of the FtsY–membraneinteraction in Archaea. Moreover, the steps leading to deliveryofSRPtothearchaealmembranehaveyettobedescribed.Towards an understanding of the membrane interaction of ... the ability of either FtsY or the FtsY NG domain to bind to the membrane, with ﬂoatation of the proteins to the uppergradient fractions occurring to similar degrees both prior to and following ... andattributed to the amino acid composition of the acidicdomain (A domain) of the protein [25,34]. Moreover, the aberrant behavior of halophilic proteins in SDS/PAGE iswell known, resulting from the...

Báo cáo khoa học: Thermal behaviour of cubic phases rich in 1-monooleoyl-rac-glycerol in the ternary system docx

... Within a region of a cubicphase in the diagram an increase in the temperaturegenerally results in a decrease in the size of the unit cell.This is interpreted to be an effect of an increase in ... present in the MO bilayer, the shorter tail will only affect the part of the MO molecule from the head group to the double bond, leaving the rest of the MO hydrocarbon chainfree, while introducing ... structure of membrane proteins isone of the cornerstones in obtaining a better understanding of their function in the biological cell. For larger proteins the most important method for achieving this...

Báo cáo khoa học: Oxygen control of nif gene expression in Klebsiella pneumoniae depends on NifL reduction at the cytoplasmic membrane by electrons derived from the reduced quinone pool doc

... fragment from K. pneumoniae M5a1 intopBSK+vector creating pRS167, followed by insertion of thetetracyclineresistancecassetteintotheEcoRV site of fdnG fragment resulting in pRS177. The 3.2-kb ... transferred from DMNH2 to the FAD-cofactor of NifL (Fig. 4A). The reduction of NifL-bound FAD by aquinol derivative was conﬁrmed using menadiol that alsoresulted in reduction of the ﬂavin cofactor ... Barrett, J., Dixon, R. & Austin, S. (2001) Protein–protein interactions in the complex between the enhancer bindingprotein NifA and the sensor NifL from Azotobacter vinelandii.J. Bacteriol. 183,...

Báo cáo khoa học: Crystal structure of RNase A tandem enzymes and their interaction with the cytosolic ribonuclease inhibitor potx

... of RNase moleculeson the cell surface), thereby favoring their endocytosis[19]. The stoichiometry of RI binding to RATEs and the role of the RI–RATE complex in the in vitro inacti-vation of ... Unfortunately, the cytotoxicity of these RNasesis limited by several factors at the cellular level [8],including restricted internalization into the cell, release from the endosomes, and inhibition by the ... excluded by the modeling studies. However, the linker keeps the two RNase A entities close to eachother not only in the crystal but also in solution,thereby supporting their interactions. As the intra-cellular...

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