Báo cáo khoa học: Solution structure of the catalytic domain of RICH protein from goldﬁsh pot

Tài liệu Báo cáo khoa học: Dimer asymmetry and the catalytic cycle of alkaline phosphatase from Escherichia coli doc

... to the magnesium binding site M3 [4].Fig. 4. The influence of Mg2+on the kinetic properties of APase from E. coli. The influence of Mg2+on the kinetic properties of APase from E. coli inthepresenceofaZn2+to ... catalysis of bothsubunits.Model representation of the catalytic cyclefor APase from E. coliA model describing the catalytic mechanism of APase from E. coli, based on the results of the kinetic ... difference between the subunits with respect fortheir affinity for the substrate or the product (the differenceFig. 3. The influence of Zn2+on the kinetic properties of APase from E. coli. Catalytic...

Báo cáo khoa học: Conserved residues in the N-domain of the AAA+ chaperone ClpA regulate substrate recognition and unfolding pdf

... forvarious adaptor proteins [6–10]. ClpA consists of threedomains: an N -domain and two ATP-binding domainsreferred to as the D1 and D2 domains. Interestingly,deletion of the N -domain from ClpA not ... that the N -domain controls bind-ing of ClpA to ClpP [14]. Interestingly, there is alsoconsiderable debate regarding the role of the ClpBN -domain (which shares a common fold with the N -domain of ... betweenClpS and the N -domain of ClpA. Consequently, dra-matic changes to the structure of the N -domain of ClpA would inhibit ClpS binding and thereby ClpS-dependent degradation of this substrate....

Tài liệu Báo cáo khoa học: Solution structure of hirsutellin A – new insights into the active site and interacting interfaces of ribotoxins docx

... mecha-nism of transphosphorylation, which implies the ABFig. 3. Stereo diagram of the active center of HtA. (A) Superposition of the active-siteresidues of the 20 conformers of the solu-tion structure ... electrostatically with the othercharged groups. In the NMR structures, this sidechain of D40 is a short distance (£ 3A˚) from the sidechains of H42 and R95. Finally, the aromatic ring of F126, placed ... second-ary structure elements. As in other ribotoxins, theseABCFig. 2. Representation of the 3D structure of HtA in solution. (A) Superposition of the 20 best structures obtained in this work(PDB...

Tài liệu Báo cáo khoa học: Solution structure of the matrix attachment region-binding domain of chicken MeCP2 ppt

... cluster in these two regions. Know-ledge of the structure of a larger portion of MeCP2including the transcriptional repression domain wouldresolve these speculations and clarify the role of the helical ... [15]. The solution structure of the MBD of rat (r)MeCP2 hasrecently been determined [17]. The MBD adopts a wedge-shaped structure, composed of an antiparallel b-sheet onone face of the wedge ... one-turn helix on the other face. It is thoughtthat the two inner strands of the b-sheet lie within the majorgroove of the DNA and that a hydrophobic pocket formedby the side chains of Y123 and...

Báo cáo khoa học: Solution structure of the catalytic domain of RICH protein from goldﬁsh pot

... al. Solution structure of the RICH catalytic domain FEBS Journal 274 (2007) 1600–1609 ª 2007 The Authors Journal compilation ª 2007 FEBS 1609 Solution structure of the catalytic domain of RICH protein from ... close to itsFig. 2. Structure of goldﬁsh RICH catalytic domain. (A) Stereo view of the backbone superposition of 10 lowest energy structures of the RICH catalytic domain. The superposition was ... similarity of the structures. The lowest-energy structure from the RICH NMR ensemble is used for the overlay. (D) The surface of the RICH catalytic domain shows several negatively charged patches of...

Báo cáo khoa học: Solution structure of the active-centre mutant I14A of the histidinecontaining phosphocarrier protein from Staphylococcus carnosus ppt

... towards the interior of the protein. The space that in the wild-typemolecule is occupied by the large hydrophobic side chain of Fig. 3. Structure ensemble of HPr(I14A). The average structure of the 10 ... for the calculations. Therefore, we recalculated the structure of the w ild-type protein on the b asis of the restraints usedpreviously [15] with the same protocol used here for the mutant protein. ... wascomputed as the ratio of the s tandard deviations of the chemical shifts of the amide nitrogen and p roton nuclei.ResultsDetermination of the three-dimensional structure of HPr(I14A)To allow the comparison...

Báo cáo khoa học: Solution structure and backbone dynamics of the XPC-binding domain of the human DNA repair protein hHR23B docx

... further our knowledge withrespect to the mechanisms of action of these proteins,it is crucial that we deﬁne the precise boundaries of the STI1-homologous domain and compare the struc-tures of ... N-terminal part of XPCB–hHR23BABCFig. 2. NMR structure of the XPCB domain of hHR23B. (A) Stereoview of the 12 superimposed structures of XPCB–hHR23B. All Pro resi-dues conserved among the STI1 homologs ... segment of the human Stch (hStch) protein can bind to the STI1-homologous domain (Fig. 1B) of the Chap1(hPLIC-1) protein [24]. Therefore, we performedsequence alignment with the hHR23B-binding domain of...

Tài liệu Báo cáo khoa học: Solution structure of crotamine, a Na+ channel affecting toxin from Crotalus durissus terriﬁcus venom docx

... fact, in the structures of Crt, the side-chain of Phe 25 is close enough to the amide proton of Lys38 to induce a ring current effect.Description of the toxin structure and potential surface The ... reported in Fig. 3A,and the analysis of the / and w angle distribution allowed the identiﬁcation of the type and boundaries of the secondary structure elements. Crt consists of three antiparal-lel ... )1topology [47] of the triple-stranded antiparallel b-sheet wasFig. 3. Summary of data used in the determination of the secondary structure of crotamine. (A) Primary sequence of Crt with the sequential...

Tài liệu Báo cáo khoa học: Nucleolin/C23 mediates the antiapoptotic effect of heat shock protein 70 during oxidative stress pptx

... the condensation and fragmentation of theirnuclei. The percentage of apoptotic cells was calculated as the number of apoptotic cells divided by the total number of cells counted. A minimum of ... stilllargely unknown. The aim of the current study was to investigate the potential involvement of nucleolin ⁄ C23 in the antiapoptotic mechanism of Hsp70. We found that primary cultures of neonatal rat ... Xiao X (2009)ATP-binding domain of heat shock protein 70 is essen-tial for its effects on the inhibition of the release of the second mitochondria-derived activator of caspase andapoptosis...

Tài liệu Báo cáo khoa học: Comparative studies on the functional roles of N- and C-terminal regions of molluskan and vertebrate troponin-I pdf

... volumes of 5M urea solution and then run on SDS ⁄ PAGE. Lanes a and d, in the absence of both TnC and Ca2+; lanes b and e, in the presence of TnC and the absence of Ca2+; lanes c and f, in the ... cation. Therefore,this interaction potentially participates in both the Ca2+-dependent activation of the contraction and the maintenance of structural integrity of the troponincomplex in the ... [26]. The initiator Met at the N-terminus was removed by the bacterial cell for all these proteins except forRTnI96)181.Inhibition of Mg-ATPase of actomyosinby TnI-fragments The inhibition of...

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