... 2007 FEBS
Molecular imprinting of cyclodextrin glycosyltransferases
from Paenibacillus sp. A11 and Bacillus macerans with
c -cyclodextrin
Jarunee Kaulpiboon
1,2
, Piamsook Pongsawasdi
3
and Wolfgang ... of the Paenibacillus sp. A11 and
Bacillus macerans cyclodextrin glycosyltransferases towards the preferential
formation of c -cyclodextrin (CD
8...
... some of
the key findings that have led to our current under-
standing of the molecular mode of action of glypho-
sate and the molecular basis for glyphosate resistance.
Structure and function of ... binds
with its phosphonate group ligated by charge interactions with Arg21 and Arg111, and its carboxyl group in contact with Arg73. The short-
ness of the hydrogen bo...
... conserved and positioned
within the loop structure connecting helices I and J
(Fig. 7A). The loop region consists of 10 amino acids,
seven of which are conserved within the species
(Fig. 2C) and two of ... because of its unusual odour and prepupal lethality.
Here, we report the identification and characterization of the gene responsi-
ble for the sku mutant. Because of it...
... (Invitrogen). Double-stranded
cDNA was digested with AluI, HaeIII, DraI and SspI and
then fractionated on a 2% agarose gel. Fragments ranging
from 50 to 150 bp were purified from the gel and ligated to
the ... both Box A and B, NRE(A
–
B
–
). (C) Qualitative
analysis of LacZ reporter activation in the interaction of APUM-2
and APUM-7 with NRE WT and NRE mutants. The iro...
... and that all the studied polysaccharides make sev-
eral hydrogen bonds with the Asp99, Arg126, Asp128
and Asp146 amino acids and, in the case of the larger
ligands, with Asp51 as well. Most of ... acquired with 0.5, 1.0, 2.0, 3.0 and 6.0 equivalents of
CaCl
2
, respectively. All the spectra were acquired at
400 MHz, with 128 scans and a spectral width of
6636.36 Hz, c...
... synovial flu-
ids and synovial tissue specimens of patients with RA,
and cultured FLS derived from either RA or OA
patients are capable of producing MIP-3a ⁄ CCL20 in
response to IL-1b and TNF-a in ... release of
mediators of inflammation, cell proliferation and
angiogenesis. To date, several animal studies and
human studies have shown the biological efficacy of
specific a...
... in
53% of synovial samples from arthritic joints, in 12%
of blood samples from RA patients and in 16% of
blood samples from patients with systemic lupus eryth-
ematosus [18]. However, data from ... 0.5–1% of the popu-
lation and causes progressive joint destruction that
leads to the restriction of activities of daily living and
deterioration of quality of life....
... TNF-a,
CD40 ligand and Fas ligand, etc. (Fig. 3). Ligand bind-
ing of various receptors results in the activation of
phospholipase C (PLC), the release of inositol 1,4,5-tri-
phosphate (IP
3
), and a ... great variety of
organs and cells. One of the roles of Ca
2+
is to regulate
calcineurin, which in turn dephosphorylates and
induces the nuclear localization of the cytop...
... Kobayashi
4
and Koichiro Kishi
3
1
Division of Medical Genetics and Biochemistry and
2
Division of Legal Medicine, Faculty of Medical Sciences, University of Fukui,
Japan;
3
Department of Legal ... comprehensive comparisons of the enzymes
from lower and higher vertebrates: the biochemical
and molecular characterizations of mammalian [ 11–16],
avian [ 17], reptilian...
... Stereoview of the catalytic cleft of Hyb-24DN.
Fig. S2. Stereoviews of surface structure of Ald-bound
and unbound Hyb-24DNY.
Fig. S3. Stereoview of the catalytic cleft of Hyb-
24DNY and class ... Dai-ichiro Kato
1
and Seiji Negoro
1
1 Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo, Japan
2 Department of Life Scien...