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Báo cáo khoa học: Acidic extracellular pH increases calcium influx-triggered phospholipase D activity along with acidic sphingomyelinase activation to induce matrix metalloproteinase-9 expression in mouse metastatic melanoma pot

Báo cáo khoa học: Acidic extracellular pH increases calcium influx-triggered phospholipase D activity along with acidic sphingomyelinase activation to induce matrix metalloproteinase-9 expression in mouse metastatic melanoma pot

Báo cáo khoa học: Acidic extracellular pH increases calcium influx-triggered phospholipase D activity along with acidic sphingomyelinase activation to induce matrix metalloproteinase-9 expression in mouse metastatic melanoma pot

... 3183 Acidic extracellular pH increases calcium in ux-triggered phospholipase D activity along with acidic sphingomyelinase activation to induce matrix metalloproteinase-9 expression in mouse metastatic melanoma Yasumasa ... attenu-ated matrix metalloproteinase-9 induction but did not affect calcium in ux.We found that acidic sphingomyelinase activity was induced by acidic extracellular pH and that the specific acidic sphingomyelinase ... sphingomyelinase inhibitors(perhexiline and desipramine) and siRNA targeting aSMase ⁄ smpd1 couldinhibit acidic extracellular pH- induced matrix metalloproteinase-9 expres-sion. BAPTA-AM reduced acidic...
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Tài liệu Báo cáo khoa học: P25a ⁄ TPPP expression increases plasma membrane presentation of the dopamine transporter and enhances cellular sensitivity to dopamine toxicity pptx

Tài liệu Báo cáo khoa học: P25a ⁄ TPPP expression increases plasma membrane presentation of the dopamine transporter and enhances cellular sensitivity to dopamine toxicity pptx

... resolved. To ensure that the flotation was due to binding to light vesicles, 1% Triton X-100 and 1% SDS were added to dissolve themembranes, as demonstrated for BIP, synaptophysin and biotinylated ... p25a p25aDN, p25aDC and the p25a core, corre-sponding to residues 44–156, were cloned into pET1 1d vector, expressed in E. coli and purified. The purified proteins were subjected to reducing SDS ⁄ ... conditionwas determined as the mean values for the contents of threeindividual wells extracted in 0.1 m NaOH and measuredusing the bicinchoninic acid method.DA toxicity assayDose-dependent...
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Tài liệu Báo cáo khoa học: The effect of small molecules in modulating the chaperone activity of aB-crystallin against ordered and disordered protein aggregation pdf

Tài liệu Báo cáo khoa học: The effect of small molecules in modulating the chaperone activity of aB-crystallin against ordered and disordered protein aggregation pdf

... aB-crystallin.Reduction-induced aggregation of the insulin B-chainLight scattering due to DTT-induced amorphousaggregation and precipitation of the insulin B-chaincommenced after 10 min and reached ... aB-crystallin protects against theDTT-induced aggregation of insulin, and this activity is enhanced by Arg-HCl. Insulin(, 0.25 mgÆmL)1) was incubated at 37 °C in 50 mM phosphate buffer, pH 7.2, with 10 ... increase the activity of aB-crystallin against DTT-induced precipi-tation of insulin at intermediate and high concentra-tions, and it also increased the activity of aB-crystallin in preventing the...
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Báo cáo khoa học: A new approach for distinguishing cathepsin E and D activity in antigen-processing organelles pdf

Báo cáo khoa học: A new approach for distinguishing cathepsin E and D activity in antigen-processing organelles pdf

... Preincuba-tion of CatE with the antibody showed a dose-depend-ent inhibition of antibody binding (IC50¼ 48.6 ng;Fig. 2C). Increasing concentrations of CatD did notaffect antibody binding. This experiment ... CatD is reported to beinvolved in processing MHC II-associated invariantchain [24] in antigen processing and presentation[25,26]. CatE is also reported to be involved in antigenprocessing ... After deple-tion of CatE by immunoprecipitation, the remaining activity is due to CatD, and the decrease in activity can be assigned to CatE. The activity ofCatE and CatD in cytosolic, endosomal...
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Tài liệu Báo cáo khoa học: Hypothalamic malonyl-CoA and CPT1c in the treatment of obesity pptx

Tài liệu Báo cáo khoa học: Hypothalamic malonyl-CoA and CPT1c in the treatment of obesity pptx

... this involves storing energy as fat duringperiods of an abundant food supply to hedge againstperiods of food shortage. Today, humans have pushedstorage too far, to the point of widespread obesity.Although ... preferable to starvation, this statefrequently leads directly or indirectly to serious pathol-ogies including diabetes and heart disease. Interven-tions to diminish adiposity beyond diet and exercisewould ... the ectopic expression of malonyl-CoA decarboxylase in the hypothalamus is sufficient to pro-mote feeding and adiposity. Based on these and other findings, metabolicintermediates in fatty acid biogenesis,...
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Tài liệu Báo cáo khoa học: Proteolytic processing regulates pathological accumulation in dentatorubral-pallidoluysian atrophy pdf

Tài liệu Báo cáo khoa học: Proteolytic processing regulates pathological accumulation in dentatorubral-pallidoluysian atrophy pdf

... stud-ies, the human control and DRPLA brain tissues were fixed in 10% formalin and embedded in paraffin. The sectionsfrom the brain tissues were immunostained with ATN1antibodies as described ... and Gerontology, Aichi,JapanIntroductionThe polyglutamine (polyQ) diseases are a group ofhereditary neurodegenerative disorders that includeHuntington’s disease (HD), dentatorubral-pallidoluy-sian ... experimentsinvolving human subjects were undertaken with the under-standing and written informed consent of each individual.The NCGG Institutional Review Board approved theexperiments involving human...
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Tài liệu Báo cáo khoa học: Roles of heat shock factors in gametogenesis and development pptx

Tài liệu Báo cáo khoa học: Roles of heat shock factors in gametogenesis and development pptx

... locali-zation within the developing neural tube [3,62,121]. In addition, mHSF2 is associated with DNA binding in the developing brain, including cortex, striatum,olfactory bulbs and mesencephalon, ... before and after birth in mouse brains[122,124]. No HSF4 DNA-binding activity wasreported in the developing mouse brain so far. How-ever, Hsf4b is expressed in the adult mouse brain,including ... mitochondrialdysfunction, in conjunction with markedly increasedproduction of reactive oxygen species [27,34]. In line with findings in the heart and kidney [35,36], andtogether with the down-regulation...
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Tài liệu Báo cáo khoa học: A role of miR-27 in the regulation of adipogenesis ppt

Tài liệu Báo cáo khoa học: A role of miR-27 in the regulation of adipogenesis ppt

... its inhibitoryeffects at or before the adipogenic commitment stageand that the IDM-induced genes appear to overcomethe inhibitory effects of miR-27. In order to determine whether miR-27inhibits ... +IDM +IDM A B C D Fig. 4. Inhibition of expression of PPARc and C ⁄ EBPa in preadipocytes by miR-27 . (A) 3T3-L1 preadipocytes were transfected with miRNAs,and then induced at 48 h to ... prepared at the indicated time points for western blotanalysis. One of three independent experiments is shown. (D) 3T3-L1 cells were treated as described in (C). Total RNA was prepared at theindicated...
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Tài liệu Báo cáo khoa học: Structural effects of a dimer interface mutation on catalytic activity of triosephosphate isomerase The role of conserved residues and complementary mutations pptx

Tài liệu Báo cáo khoa học: Structural effects of a dimer interface mutation on catalytic activity of triosephosphate isomerase The role of conserved residues and complementary mutations pptx

... replaced by Cys in order to introduce a symmetry-related disulfide bond with the Cys residue at position 13 of the other sub-unit [25,26], yielding a covalently bridged dimer. Theoxidized and reduced ... contained 100 mm triethanol-amine, 5 mm EDTA, 0 .5 mm NADH and a-glycerolphosphatedehydrogenase (20 lgÆmL)1) and 0.10–3.0 mm glyceralde-hyde 3-phosphate. Enzyme activity was determined bymonitoring ... spectroscopy.Protein concentrations were determined by the Bradfordmethod [53] using BSA as a standard.Enzyme activity The enzyme activity of TIM was determined by the conver-sion of glyceraldehyde 3-phosphate...
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Tài liệu Báo cáo khoa học: Shaped by the environment – adaptation in plants Meeting report based on the presentations at the FEBS Workshop ‘Adaptation Potential in Plants’ 2009 (Vienna, Austria) pdf

Tài liệu Báo cáo khoa học: Shaped by the environment – adaptation in plants Meeting report based on the presentations at the FEBS Workshop ‘Adaptation Potential in Plants’ 2009 (Vienna, Austria) pdf

... stressed the importance oflight-induced protein degradation in phytochrome-con-trolled signalling and showed data on mutants, isolated in a custom-designed genetic screen, that are impaired in ... showed that the CUL4–DDB1–DDB2 complex plays a role in nucleotide excision repairof UV-C-induced DNA damage and that this activity iscontrolled by the ATR kinase [68]. In addition,preliminary ... one hand, requiringsunlight that contains UV radiation to undergo photo-synthesis and, on the other hand, of having to ensurethat UV radiation does not induce irreversible DNAdamage. Molinier...
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