Báo cáo khoa học: Inter-flavin electron transfer in cytochrome P450 reductase – effects of solvent and pH identify hidden complexity in mechanism potx
Báo cáo khoa học: Inter-flavin electron transfer in cytochrome P450 reductase – effects of solvent and pH identify hidden complexity in mechanism potx
... transfer in cytochrome P450 reductase FEBS Journal 275 (2008) 454 0–4 557 ª 2008 The Authors Journal compilation ª 2008 FEBS 4557 Inter-flavin electron transfer in cytochrome P450 reductase – effects of ... The role of cysteine 566 in catalysis and cofactor binding. J BiolChem 266, 1997 6–1 9980.26 Shen AL & Kasper CB (1995) Role of acidic residues in the interaction of NADPH cytochrome P450 oxidore-ductase ... (1994) Kinetic mechanism forthe model reaction of NADPH cytochrome P450 oxidoreductase with cytochrome c. Biochemistry 33,1201 2–1 2021.S. Brenner et al. Electron transfer in cytochrome P450 reductase FEBS...
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Báo cáo khoa học: Interflavin electron transfer in human cytochrome P450 reductase is enhanced by coenzyme binding docx
... folded domains: an FAD- and NADPH-bindingdomain related to ferredoxin-NADP+ reductase, a flavo-doxin-like FMN-binding domain and a connecting ÔlinkerÕdomain. In addition to the cytochromes P450, ... kineticexperiments to include studies of internal electron transfer in the presence of NADPH, NADH and fragments of nicotinamide coenzymes. We show that binding of the2¢-phosphate of NADPH, and to a lesser ... reduc-tion in rate of interflavin electron transfer in NADH-reduced CPR thus suggests a role for coenzyme binding, and in particular for interactions made by the 2¢-phosphate of NADPH, in modulating interdomain...
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Báo cáo khoa học: N-Glycosylation is important for the correct intracellular localization of HFE and its ability to decrease cell surface transferrin binding pptx
... regulation of transferrin bindingPrevious reports have established that in certain celltypes HFE acts to regulate intracellular iron levels bydecreasing the binding of transferrin to transferrinreceptors ... striking decrease in cell-surface bind-ing of transferrin, and that little or no reduction in transferrin binding was observed in cells expressing theHFE-C282Y mutant (Fig. 7A).ABFig. 5. Intracellular ... (2009) In vitrobinding of HFE to the cation-independent mannose-6phosphate receptor. Blood Cells Mol Dis 43, 18 0–1 93.44 Elbein AD (1987) Inhibitors of the biosynthesis and processing of N-linked...
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Báo cáo khoa học: Testosterone 1b-hydroxylation by human cytochrome P450 3A4 pdf
... [26] using a singletestosterone concentration of 100 lM. – Indic ates rate < 0.1 min)1. P450 Rate (min)1)1b-OH 2b-OH 6b-OH 15b-OH1A1 – – 1.1 ± 0.1 – 1A2 – – 6.7 ± 0.3 – 1B1 – – 7.7 ... baculovirus-infected insect cells contain-ing 4 pmol of P450 3A4. (Varying amounts of P450 wereused because of differences in rates of the systems, in orderto maintain linearity of product formation vs. ... from solvent A (95% CH3CN, 5% H2O, v/v) and solvent B (H2O), u sing the schedule as follows: 0–5 .5 min,75% (v/v) solvent B; 5. 5–1 2 min, 75% to 64% solvent B;1 2–2 4 min, 64% (v/v) solvent...
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Báo cáo khoa học: A steady-state competition model describes the modulating effects of thrombomodulin on thrombin inhibition by plasminogen activator inhibitor-1 in the absence and presence of vitronectin ppt
... plasminogen activator inhibitor and vitronectin and evidence that the bovine inhibitor binds to athrombin-derived amino-terminal fragment of bovine vitronectin.Biochim. Biophys. Acta 1078, 2 3–3 0.24. ... overlapping bindingsite on thrombinAt this point, the mechanism of the inhibitory effect of TMon the interaction between PAI-1 and thrombin remains tobe elucidated. To that end, binding of PAI-1 ... asdemonstrated in this study, binding of TM does stericallyhinder PAI-1 binding to thrombin (–VR1tPA). Therefore,the binding of PAI-1 to thrombin either involves a smallpart of the VR1 loop that is physically...
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Tài liệu Báo cáo khoa học: A novel electron transport system for thermostable CYP175A1 from Thermus thermophilus HB27 doc
... 1748 6– 17500.30 Sielaff B & Andreesen JR (2005) Kinetic and bindingstudies with purified recombinant proteins ferredoxin reductase, ferredoxin and cytochrome P450 comprisingthe morpholine mono-oxygenase ... potassium phosphate buffer (pH 6.3) containing10% glycerol and 100 mm KCl, CYP175A1 was eluted witha linear gradient of 10 0–6 00 mm KCl in 50 mm potassiumphosphate buffer (pH 6.3) containing 10% ... composed of an iron–sulfurprotein and an FAD-containing NAD(P)H-dependent reductase [12]. Eukaryotic cytochrome P450s utilize theclass II system, composed of an NADPH-dependent reductase containing...
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Báo cáo khoa học: "Simultaneous English-Japanese Spoken Language Translation Based on Incremental Dependency Parsing and Transfer" pot
... In addition, we outline an approach of si-multaneous machine translation utilizing linguis-tic phenomena, flexible word order, and inversion,characterizing Japanese speech.2.1 Difference of ... English speech transcriptinto Japanese incrementally. It is composed of three modules: incremental parsing, transfer and generation. In the parsing module the parser deter-mines the English dependency ... of （2.1）is input. In the follow-ing subsections we explain each module, referringto Fig. 6.4.1 Incremental Dependency ParsingFirst, the system performs POS tagging for inputwords and chunking (c.f....
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Báo cáo khoa học: A fluorescence energy transfer-based mechanical stress sensor for specific proteins in situ pdf
... proteins.a-Actinin–stFRET (A), a-actinin–GFP (B), filamin A–stFRET (C), filam- in A–CFP (D), spectrin–stFRET (E) and spectrin–CFP (F) in 3T3 fibro-blast cells; Collagen-19–stFRET (G) and COL-19–GFP (H) in C. ... stretching of the coiled-coil myosin II [44]. Schweiger et al. [45]obtained a three-phase force–distance relationship: alinear phase of $ 1mNÆm)1, a plateau of $ 25 pN, and a wormlike chain phase ... spectrin(Fig. 10E), filamin A (Fig. 10C), and a -actinin(Fig. 10A), and expression systems including HEK-293,3T3 and C. elegans, and the insertion locations wereoptimized to obtain protein distributions...
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Tài liệu Báo cáo khoa học: Diol dehydratase-reactivating factor is a reactivase – evidence for multiple turnovers and subunit swapping with diol dehydratase pdf
... cobalamin at 0 min (thick solidline), 30 min (thin solid line), 60 min (thin long-dashed line),120 min (thin short-dashed line), 240 min (thin dotted line) and 360 min (thick dotted line) of incubation, ... model of damagedcofactors) for free adeninylpentylcobalamin (AdePeCbl)(an inactive coenzyme analog containing the adeninering in the upper axial ligand; a model of intact coen-zyme, AdoCbl) in ... min of incubation in the absence of ATP and Mg2+(thicksolid line) or without the reactivating factor in the presence of ATP and Mg2+(thick dotted line). (C) Time course of the reactivating...
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Tài liệu Báo cáo khoa học: Erythrochelin – a hydroxamate-type siderophore predicted from the genome of Saccharopolyspora erythraea docx
... C5-domain,catalyzing the intramolecular nucleophilic attack of the L-hOrn3a-amino group onto l-haOrn4. Takinginto account that the synthetases involved in the bio-synthesis of the DKP-inheriting ... (solvent B) at a flow rate of 0.3 mLÆmin)1: lin-ear increase from 0% B to 50% within 20 min followed bya linear increase to 95% B in 5 min, holding B for anadditional 5 min. This gradient was ... (solvent B) at a flow rate of 16 mLÆmin)1: lin-ear increase from 0% B to 50% within 50 min followed bya linear increase to 95% B in 5 min, holding B for an addi-tional 5 min. The wavelengths chosen...
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Từ khóa: Nghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDEPhối hợp giữa phòng văn hóa và thông tin với phòng giáo dục và đào tạo trong việc tuyên truyền, giáo dục, vận động xây dựng nông thôn mới huyện thanh thủy, tỉnh phú thọPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhNghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếTìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinThơ nôm tứ tuyệt trào phúng hồ xuân hươngThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXChuong 2 nhận dạng rui roBT Tieng anh 6 UNIT 2Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtTrách nhiệm của người sử dụng lao động đối với lao động nữ theo pháp luật lao động Việt Nam từ thực tiễn các khu công nghiệp tại thành phố Hồ Chí Minh (Luận văn thạc sĩ)BÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIĐổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt namHIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀMMÔN TRUYỀN THÔNG MARKETING TÍCH HỢP