... by:FD¼ A TÀ A N =A DÀ A NWhere A Nis the absorbance of the protein sample at 30 °C, A Dis the absorbance of the sample at 80 °C and A Tis the absorbance of the protein sample at different ... cuvettes and equilibrated at 30 °C to obtain the baseline. The samples were heated from 30 °Cto90°Cat the rate of 1 °CÆmin)1using a programmable thermalcontrol unit. The absorbance change in each ... tetrahydrofolate.J Mol Biol 296, 155–168.12 Trivedi V, Gupta A, Jala VR, Saravanan P, Rao GS,Rao NA, Savithri HS & Subramanya HS (2002) Crystalstructure of binary and ternary complexes of...