Báo cáo khoa học: Structural mobility of the monomeric C-terminal domain of the HIV-1 capsid protein pptx

Tài liệu Báo cáo khoa học: Structural basis for cyclodextrin recognition by Thermoactinomyces vulgaris cyclo⁄maltodextrin-binding protein ppt

... at the entrance of the cleft of EcoMBP, but the distancebetween Od2 of Asp209 and Ng2 of Arg344 is only10.2 A˚.InTliTMBP, the cleft is the smallest among the three proteins (Fig. 7C). The ... half-buried, and the form of the cleft is markedlydifferent from those of TvuCMBP and EcoMBP.These observations suggest that the structure of the N -domain part and the capacity of the sugar-bindingsite ... all of the conformations are the closed form, were compared(Fig. 7A–C). The cleft of the sugar-binding site of TvuCMBP is the widest among the three sugar-bindingproteins (Fig. 7A). Although the...

Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

... respectively, of that of HPRT. This low activitycould be explained by the fact that PRTFDC1 has a Gly in the position of the proposed catalytic Asp of HPRT. In PRTFDC1, a water molecule at the position of ... strength [20].In the present study, we report the structural andfunctional characterization of the human HPRThomolog PRTFDC1. To shed light on the potentialfunction of PRTFDC1, the protein was ... andregulators of other enzymes in the human nucleotidemetabolism (Table S1). The library can then be seen toconstitute the speciﬁc metabolome of the nucleotidemetabolism, which would be the most...

Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of ﬂavoproteins: electron transfer through the nitric oxide synthase ﬂavoprotein domain pdf

... hinge domains The composition of the two hinges that connect the FMN subdomain in NOS enzymes (H1 and H2 inFig. 5) defines the allowable movements of the FMNsubdomain and thus controls the FMN–NOSoxy ... inNOS, the second electron is provided to the heme-dioxy species by a bound H4B cofactor rather than by the flavoprotein domain [16]. The H4B radical is thenreduced within the enzyme by the ... alsoinfluence the rate of electron import into the FMN sub- domain and the resulting formation of FMNH2. The extent to which KeqA or the rate of FMNH2formationinfluences electron flux through the...

Tài liệu Báo cáo khoa học: Structural effects of a dimer interface mutation on catalytic activity of triosephosphate isomerase The role of conserved residues and complementary mutations pptx

... nature. The growing body of sequence and structural data on these well-studiedenzymes affords an opportunity to evaluate the conse-quences of mutations. In the case of TIM, only nine of the 220–250 ... methodfor the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. AnalBiochem 72, 248–254.54 Oesper P & Meyerhof O (1950) The determination of triose ... to the WT* enzyme. There are two possiblereasons for the low activity of the Y74W* mutant:(a) introduction of the bulkier residue at the interface inplace of a tyrosine may destabilize the...

Tài liệu Báo cáo khoa học: Helix mobility and recognition function of the rat thyroid transcription factor 1 homeodomain – hints from 15N-NMR relaxation studies pdf

... evidencesupporting a structural interruption of the geometry of the recognition helix for either Antp or the TTF-1HD, the anomalous amide exchange pattern and the NOE connectivity data of the C-terminal ... conﬂict with the global interpretation of the data. With the exclusion of the N-terminal octapeptidyl and C-terminal nonapept-idyl fragments of Glu17, Leu34 and Lys46, the average of the {1H}–15N ... hydrodynamicradius of 1.98 nm for the TTF-1 HD under the condi-tions of this study, i.e. very close to the mean radius of the NMR structure of the molecule (1.94 nm) [4]. The conclusions inferred...

Tài liệu Báo cáo khoa học: Structural insights into the substrate speciﬁcity and activity of ervatamins, the papain-like cysteine proteases from a tropical plant, Ervatamia coronaria ppt

... proton from the catalytic Cys pres-ent at the N-terminus of the helix to the His of the dyad [19,50,51]. The ﬁrst stage of catalysis is mediatedby the highly active thiolate ion of the Cys. Biochemi-cal ... erva-tamin-A, the P3 moiety of both the molecules of the asymmetric unit runs along the extended backbone of residues 65–64 and is partly exposed to the solvent(Fig. 2B). On the other hand, the P3 ... at the bottom of the subsite is conservedin ervatamins. Compared to a Ser in the equivalentposition of papain, the role of Leu here is to restrict the size and depth of the S2 subsite. The...

Tài liệu Báo cáo khoa học: Structural and functional speciﬁcities of PDGF-C and PDGF-D, the novel members of the platelet-derived growth factors family docx

... urchin EGF-like protein andbone morphogenic protein 1 [16]. These proteins areoften referred to as the prototype CUB domains. Like the prototype CUB domains, the PDGF-C and -DCUB domains span ... the prototypic CUB domains, respectively (Fig. 2) [8]. The CUB domains of PDGF-C and -D share  55%sequence identity. CUB domains are assembled as aFig. 2. The CUB domain. The CUB domains of ... oppositeedges of the domain. As both the PDGF-C and -DCUB domains contain only two cysteines [7] which,compared to the classical CUB domains, are the twomost-C-terminally located cysteines, the CUB domainsof...

Tài liệu Báo cáo khoa học: Structural characterization of Ca2+/CaM in complex with the phosphorylase kinase PhK5 peptide pdf

... motif’, a structurally uncharacterized motif.Fig. 2. An overview of the PhKc domain structure. The ﬁrst 296residues of the subunit encode a protein kinase domain. The struc-ture of the constitutively ... these structures a C-terminal extension to the protein kinase folds backon the kinase domain and interferes with the substratebinding sites. In the case of the titin and twitchinkinases, the ... [14,15]. The protein consists of twodomains each encoding two EF-hand motifs separatedby a linker region that imparts a high degree of conformational ﬂexibility. In the presence of Ca2+ions the protein...

Báo cáo khoa học: Structural and functional aspects of unique type IV secretory components in the Helicobacter pylori cag-pathogenicity island potx

... about the function of the protein. The Fig. 1. (A) Cartoon representation of CagZ protein fold. The L-shape is clearly visible.(B) Two views of the electrostatic potentialsurface of CagZ. In the ... side of the peanut. The results of the crystallo-graphic model of CagS do not show any clear evidence of architectural similarity to other known structures,with the exception of a weak structural ... presence of Cu(II), the protein shows the presence of the ion coordinatedin a small cavity of the surface at the polar opposites of each monomer, close to another dimer present in the crystal packing...

Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx

... C-lobe domain containing 1) [9]. The KIND domain in theseproteins is localized to the N-terminal region, and theirspeciﬁc functional domains are located in the C-termi-nal region. The C-lobe of protein ... central domain of MAP2contain the v-KIND binding core moduleMAP2 consists of three main structural domains: the cAMP-dependent protein kinase regulatory subunitRII binding domain, the central domain ... (Fig. S2B). Taken together, these resultsreveal the speciﬁc protein protein interaction between the v-KIND KIND2 domain and the middle CDregion of MAP2.To narrow down the region responsible...

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