Báo cáo khoa học: Ionizing radiation utilizes c-Jun N-terminal kinase for amplification of mitochondrial apoptotic cell death in human cervical cancer cells pptx
... Journal compilation ª 2008 FEBS
Ionizing radiation utilizes c-Jun N-terminal kinase
for amplification of mitochondrial apoptotic cell death
in human cervical cancer cells
Min-Jung Kim
1
, Kee-Ho Lee
2
and ... targets for
cancer treatment.
Results
To examine the kinetics of the apoptotic cell death
induced by ionizing radiation in human c...
... and it therefore allows for cellular changes in the mating pro-
cess. Therefore, the generation of diploid cells with opposite mating-type cells can be used to screen interacting protein pairs, ... with
intact a cells. The formation of diploid cells in medium
lacking methionine and lysine depends on the affinities
of the protein pairs [12].
To verify our hypothesis that the...
... (solvent B) at a flow rate of 0.3 mLÆmin
)1
: lin-
ear increase from 0% B to 50% within 20 min followed by
a linear increase to 95% B in 5 min, holding B for an
additional 5 min. This gradient was ... 673
Determination of amino acid connectivity via
partial hydrolysis of erythrochelin
Three milligrams of erythrochelin were partially hydrolyzed
in 200 lLof6m HCl at 110 °C for 20...
... more in WAT of TG
mice than in WAT of WT mice.
Serum levels of triglyceride, glucose, leptin and
insulin, and insulin sensitivity, in TG mice
After 6 weeks of normal or HF diet, serum levels of
triglyceride, ... regulation of insulin produc-
tion in pancreatic islet cells [39–41]. Thus, the
increased insulin level seen in H-PGDS TG mice when
on the HF diet might be due...
... be a sink for Cu(I). This could
be sufficient for a redox-silencing role of MT3 for Cu.
In this context it might be interesting to search for
possible binding partners of Cu(I)
4
MT3.
Since the ... occurred in
the Zn cluster [32]. Indeed, exposure of Cu(I)
4
-
Zn
4
MT3 to air resulted in the slow formation of a
disulfide linkage by cysteine oxidation of the a-domain
and...
... were
determined by SDS-PAGE followed by silver staining.
Preparation of mouse activated protein C
Incubation of mouse protein C with human or bovine
thrombin using conditions known to work well for human
protein ... randomized. No binding was detected
in buffer lacking Ca
2+
for any of the proteins tested.
Protein binding equilibrium data (R
eq
) were fitted to a one-
site bind...
... interface [8,9]. BTB ⁄ POZ proteins are
often transcriptional regulators containing a C
2
H
2
domain for DNA binding, but can also be found in
combination with various other protein–protein inter-
action ... proteins use their BTB ⁄ POZ domain to interact
with the cullins CUL3A and CUL3B [3,4,16], we inves-
tigated what kind of protein–protein interactions were
facilitated by their MATH...
... or
mis-folding of proteins may also be a signal for ubiqui-
tination of denatured proteins that are recognized by
the appropriate ubiquitin ligase. In human cells, block-
ing of the metabolism of mis-folded ... the stability of Egd2 was
decreased in wild-type cells but not in rsp5 mutant cells
in the absence of CX (Fig. 2A). However, in the pres-
ence of CX, E...
... 8.4-fold
induction of HSF1 DNA-binding in heat-shocked
HeLa cells, and, on average, 25.8-fold induction of
HSF1 DNA-binding in heat-shocked MEFs when
compared to the untreated cells. The results are well in
line ... through the DNA-binding domain.
Stable binding requires simultaneous binding of all
DNA-binding domains in a trimer to three adjacent
nGAAn repeats. Therefore, a f...
... role in intracellular
signalling and thus in cell physiology and cellular
destruction. ROS are known to induce a wide range of
responses, depending on cell type and levels of ROS
within the cell ... 2¢,7¢-dichlorodihydrofluorescein diacetate; DR-5, death receptor-5; JC-1, 5,5¢,6,6¢-tetrachloro-
1,1¢3,3¢-tetraethylbenzimidazol-carbocyanine iodide; JNK, c-Jun N-terminal kina...