Báo cáo khoa học: Changes in acetylcholine receptor function induce shifts in muscle ﬁber type composition docx

... 1 fibers (dark stain), type 2A fibers (light stain), and type 2B ⁄ 2D fibers (intermediatestain). (B) ATPase staining at pH 9.4 identifies type 1 fibers (lightstain), and type 2 fibers (dark stain). ... identical muscle fibers in serial cross-sections. (i) Type 1 muscle fiber. (a) Type 2A muscle fiber. (b) Type 2B ⁄ 2D muscle fiber. Scale bar in (D) is 100 lm. (A)ATPase staining at pH 4.6 identifies type ... 2008 FEBS Changes in acetylcholine receptor function induce shifts in muscle fiber type composition Tae-Eun Jin1,*, Anton Wernig2and Veit Witzemann11 Abt. Zellphysiologie, Max-Planck-Institut...

Tài liệu Báo cáo khoa học: Epidermal growth factor receptor in relation to tumor development: EGFR gene and cancer ppt

... ligand-binding domain; a transmembranedomain; an intracellular tyrosine kinase domain; and aC-terminal regulatory domain [2]. The extracellulardomain is subdivided further into four domains. ... EGFR(including EGF, transforming growth factor-a, amphi-regulin and epigen); (b) those that bind to EGFR andERBB4 (including betacellulin, heparin-binding EGFand epiregulin); and (c) neuregulin ... Uponbinding its ligands, dynamic conformational changes occur in both extra-cellular and intracellular domains of the receptor tyrosine kinases, resulting in the transphosphorylation of tyrosine...

Tài liệu Báo cáo khoa học: Epidermal growth factor receptor in relation to tumor development: EGFR-targeted anticancer therapy doc

... compete with ATP for binding tothe tyrosine kinase pocket of the receptor, therebyinhibiting receptor tyrosine kinase activity and EGFRsignaling pathways (Fig. 1). Early clinical studiesshowed ... extracellular domain of the receptor, thereby inhibiting ligand-dependent EGFR signal transduction; and small-molecule tyrosine kinase inhibitors, such as geﬁtinib and erlotinib, whichtarget the intracellular ... KRAS protein is localized to the inner surface ofthe cell membrane. The binding of ligand to EGFRinduces receptor dimerization and consequent confor-mational changes that result in activation...

Tài liệu Báo cáo khoa học: Changes in purine speciﬁcity in tandem GAF chimeras from cyanobacterial cyaB1 adenylate cyclase and rat phosphodiesterase 2 pptx

... major interaction site for the adeninemoiety. In b1, an arginine residue (Ile417 in rPDE2)binds to N1 and a threonine (Ser419 in rPDE2) inter-acts with N7 and the N6amino group. The inter-actions ... cGMP binding [13], and, in PDE6, both of theamino acids corresponding to Phe433 ⁄ Asp434 in rPDE2A are required for cGMP binding [14].Our initial mutations in cyaB1 GAF B also involvedindividual ... (Phe433 ⁄ Asp434 in rPDE2) that interactswith the pyrimidine ring of the purine, either individu-ally or in various combinations in 12 different con-structs, enhanced cAMP binding but had little...

Tài liệu Báo cáo khoa học: Changes in rat liver mitochondria with aging Lon protease-like activity and N e-carboxymethyllysine accumulation in the matrix doc

... FUÆlgprotein)1Æh)1). In parallel with this decrease in activity, oxidized proteins accumulated in the matrix uponaging while the CML-modified protein content assessedby ELISA significantly increased ... been found in several mammalsincluding human tissues and cell lines [24–26]. It isresponsible for the degradation of abnormal proteins andcertain short-lived specific proteins [27,28]. In this ... 0.06Ó FEBS 2003 Protein alteration in mitochondrial matrix with aging (Eur. J. Biochem. 270) 2297ADP/O ratios obtained with succinate or glutamate indicateno change in coupling efficiency. The...

Báo cáo khoa học: Changes in speciﬁc lipids regulate BAX-induced mitochondrial permeability transition pptx

... proteinfamily that resides in an inactive state in the cytoplasmof normal cells. Following an apoptotic stimulus, BAXundergoes conformational changes [1–4], becoming amediator of the intrinsic ... blot. Protein loading wasdetermined by using anti-ANT polyclonal IgG. (C) Cytochrome c content in mitochondria (M) and in supernatants (SS) recovered after rBAXincubation. Proteins in the supernatants ... protectedagainst mPTP opening induced by rBAX (Fig. 5). It isconceivable that the cholesterol ⁄ gangliosides ratio maybe relevant to maintaining the structure of the lipidmicrodomains, in such...

Báo cáo khoa học: "Medical emergency teams: deciphering clues to crises in hospitals"

... andthe incidence of crisis recognition in hospitals. Their review ofover 2000 events revealed an increase in events at certaintimes of the day, notably near nursing handoffs and physicianrounding. ... there are norandomized clinical trials showing benefit from introduction ofMETs, many single center reports [2-4] support the notionthat timely intervention may interrupt crisis events anddecrease ... emergency teams: deciphering clues to crises in hospitalsMichael DeVitaAssociate Professor, Critical Care Medicine and Internal Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania,...

Tài liệu Báo cáo khoa học: Modulation of a-synuclein aggregation by dopamine in the presence of MPTP and its metabolite pptx

... K,Kuroiwa Y et al. (2008) RNA-binding protein TLS is amajor nuclear aggregate-interacting protein in hunting-tin exon 1 with expanded polyglutamine-expressingcells. J Biol Chem 283, 6489–6500.25 ... absence of Lewy bodies in MPTP-induced parkinsonism in humans. However,eosinophilic intraneuronal inclusions have been seen in the same region as Lewy bodies in squirrel monkeysinjected with MPTP ... urea.Effect of dopamine on MPTP and MPP+induced changes in kinetics of the aggregation ofa-synucleina-Synuclein was incubated in the presence of 100 lmMPTP, along with 50 lm dopamine. Aliquots...

Tài liệu Báo cáo khoa học: Animal models of amyloid-b-related pathologies in Alzheimer’s disease docx

... having sufﬁcient power are thereforeextremely important in minimizing the in uence ofconfounding factors arising from inherent problemsassociated with breeding APP transgenic mice andmaintaining ... time-consuming and greatly in uenced by individual handlers. IntelliCages areautomated learning cages where animals carryingtransponders are housed in groups and trained in learning corners. Each individual ... thepathogenic interaction between Ab and a-synucleinwas investigated by crossing PDGF–a-synucleinand APP-SweInd, which led to a 1.6-fold increase in a-synuclein inclusions in comparison with...

Tài liệu Báo cáo khoa học: Plasticity of laccase generated by homeologous recombination in yeast docx

... proteinutilizing the principle of protein–dye binding. AnalBiochem 2, 248–254.38 Bauer R & Rupe CO (1971) Use of syringaldazine in aphotometric method for estimating ‘free’ chlorine in water. ... variations in identity(for example ranging from 38–86% in Coprinopsis cine-rea [27]). This natural diversity within in a singleorganism can be used for protein engineering purpose. In Trametes ... sequence domain (blue cop-per-binding domain) that shares distant homology tothe single-domain cupredoxins [21,22]. The evolution-ary path from a single-domain cupredoxin to a three-domain laccase...

Xem thêm