... b
2
isoforms [13].
The present study aimed to identify peptides derived
from the amino acid sequence of the PKI55 protein to
be used as pharmacological tools. The effects of the
peptides in vitro ... peptides. The level of
PKC a, b
1
, b
2
and f isoforms was also studied.
Results
Synthesis of peptides derived from PKI55 and
their inhibitory effect on r...
... vesicles, as the blue shift
of Trp was 11 nm.
To study the contribution of electrostatic inter-
actions to the binding of both peptides with negatively
charged SUVs, titration of the peptides with ... nm, expressed as the percentage of the fluorescence of
the lipid-free peptides vs. the added lipid concentration.
The data were analysed using Graphpad softw...
... 2433
C-terminal end of helix III, pointing towards the inside
of the bundle, and Cys128 was located to the random
coil of the C-terminal end of the protein (C-terminal
to helix IV) (not shown). The long ... members of the haemerythrin family and
it is located in the central region of these proteins.
We therefore named the MCA0715-encoded protein
Methylococcus cap...
... cell–cell adhesion complex and required for the for-
mation of epithelia in the embryo and the maintenance
of the polarized epithelial structure in the adult [2]. As
a single-span transmembrane-domain ... suppressed the disruption of cell–cell
adhesion, the scattered phenotype of the cells and the
redistribution of E-cadherin into the cytoplasm
(Fig. 5C).
C
ab
c...
... independent of the thermodynamic and kinetic
properties of the binding process and is solely based on the
detectability of the peptides of interest. We have used this
method to show that binding of fosfomycin ... function of temperature [13]. The heat capacity change
(DC
p
) was obtained from the slope of Kirchoff’s plots (DH
vs. T). Analysis of the experimen...
... his system of mechanical translation of
Russian upon word-for-word translation, strip-
ping the Russian words of their endings to
identify them by their stems, which are listed
in the dictionary.
... twenty-eight pages of continuous
text
2
were analyzed for the relative positions
of the subject and the predicate. The predicate
spot was determined syntactically, b...
... through the TBP component with the
AT-rich elements but the complexes were readily disso-
ciated in the absence of TFIIIC. The TATA sequences
present elsewhere in the flanking regions of these genes
could ... to the differences in their tran-
scription levels, the dissociation of TFIIIB complexes
in the presence of heparin was examined. Because hep-
arin strips off t...
... contrast to the CD11b I domain
GST, the majority of the CD11a I domain GST was found
in the insoluble fraction of the E. coli lyzate. Modification
of the solubilization conditions and increasing the volumes
of ... providing the LLG-C4-GST
protein. These studies were supported by the University of Helsinki, the
Academy of Finland, the Sigrid Juse
´
lius Foundat...
... enzyme, the target of the present study,
did not appear at around 34 kDa, which is the mass
assigned to it as a potential MCP (see MALDI-TOF
MS analysis and below), when the extracts (either from
the ... with the aim of both perturb-
ing the MS spectrum and capturing the MCP in the
body extract. The control spectra, as well as the ‘per-
turbed’ one (by rPCI addit...
... strongly
depends on the nature of the acyl moiety [9,10]. The
fine tuning between these two features is of consider-
able importance for the development of selective
potent drugs.
The biosynthesis of the peptide ... has been
shown that the activity of these acidic lipopeptides is
significantly influenced by the length and structure of
their fatty acid moieties [9,10]....