... mutantWar1- 42 allele, four clustered mutations were found:deletion of A 228 6, T 228 7 and T 228 8, and the G 229 1Ttransversion. These four mutations caused three aminoacid changes, namely K762N, F763M and the ... loss -of- function alleles of WAR1. The strains W303-D (WT ⁄ WT), YBB21 (WT ⁄ war1D),YBB24 (WT ⁄ 28 ), YBB 22 (WT ⁄ 42) , YBB25 (war1D ⁄ 28 ), YBB26(war1D ⁄ 42) , YBB23 (28 ⁄ 42) and YBB14 (pdr12D) ... absence of the activation domain,whereas the effect of the mutations in War1- 42 on War1p function is not immediately obvious.Characterization of War1-42p and itspost-translational modificationTo...