... T7, trc andlacUV5 promoters.This supplementary material can be found in theonline version of this article.Please note: As a service to our authors and readers,this journal provides supporting ... Translationinitiation in Escherichia coli: sequences within theribosome-binding site. Mol Microbiol 6, 1219–1229.24 Mattanovich D, Weik R, Thim S, Kramer W, Bayer K& Katinger H (1996) Optimization ... commonly used BL21 strain isBL21(DE3). This strain has an insert on the chromo-some encoding the T7 RNA polymerase controlled bya lacUV5 promoter. This feature allows the use of theT7 promoter....