Báo cáo khoa học: Respective roles of the catalytic domains and C-terminal tail peptides in the oligomerization and secretory trafﬁcking of human acetylcholinesterase and butyrylcholinesterase potx

... works Respective roles of the catalytic domains and C-terminal tail peptides in the oligomerization and secretory trafﬁcking of human acetylcholinesterase and butyrylcholinesterase Dong Liang1,2, ... This indicates apredominant in uence of the catalytic domain on oligomerization. Role of the C-terminal cysteineMutation of the cysteine located at )4 from the C-ter-minus to a serine in the ... C-terminal t peptides do not in uence the catalytic activity of AChE and BChEWe examined the possible in uence of the C-terminal peptides on the AChE and BChE activities bycomparing the catalytic...

Báo cáo khoa học: Plant DNA polymerase k, a DNA repair enzyme that functions in plant meristematic and meiotic tissues docx

... protein contains a BRCT domain at the N-terminus and a Pol X domain at the C-terminal region,similar to the overall domain organization of mammalianPol k. The nucleotide sequence data reported in ... by the UPGMA method, based on the amino acidsequences of the DNA polymerase domains of OsPol k and the other X-family DNA poly-merases (B). (A) OsPol k has a BRCT(BRCA1 C-terminal) domain ... source of activating metal ions. Extension of the labeled (*) primer strand in the presence of either the correct (0.1 lM)ortheincorrect(100 lM)dNTPwasanalyzed by 8Murea and 10% PAGE and...

Tài liệu Báo cáo khoa học: Critical roles of Asp40 at the haem proximal side of haem-regulated phosphodiesterase from Escherichia coli in redox potential, auto-oxidation and catalytic control doc

... potential of the haem, rate constants o f CO, O2 and CN b inding tohaem, and CD spectra in the Soret region [15–18],suggesting that this residue is critical for maintaining the electronic states of ... exogenous ligand binding accesschannel a t t he haem distal side and/ or structure in t erms of cyanide binding in the Fe(III) complex.Redox potentials The redox state of haem is related to the PDE ... that of the wild-type protein.Additionally, spin states of the Fe(III), Fe(II) and Fe(II)-CO complexes of Asp40 mutants were similar tothose of the wild-type enzyme in that all mutant proteinswere...

Báo cáo khoa học: Different roles of two c-tubulin isotypes in the cytoskeleton of the Antarctic ciliate Euplotes focardii Remodelling of interaction surfaces may enhance microtubule nucleation at low temperature doc

... compensation. Amino acid changesadjacent to the T3 loop and within the T5 loop may in uence the binding afﬁnity and hydrolysis of GTP and ⁄ or the egress of GDP and Pi, which may in turn control the assembly ... terminated by telomeres consisting of fourrepetitions of the motif C4A4[62]. This stereotypic organi-zation facilitated obtaining the sequences of the C-terminal coding region and the 5¢- and ... participates in the assembly of the mitotic spindle of E. focardii and that both isotypes are involved in the nucleation of other microtubule structures.Finally, we examined the distribution of E....

Báo cáo khoa học: Different roles of functional residues in the hydrophobic binding site of two sweet orange tau glutathione S-transferases pdf

... to30 kDa in size. Subunits of all known GST structuresexhibit a two-domain fold, the N-terminal domain and C-terminal domain, including the highly conservedGSH-binding site (G-site) and the more ... differ in only threeamino acids: the triad Arg89, Glu117 and Ile172 found in the isoform GSTU1 is replaced by the triad Pro89,Lys117 and Val172 in the GSTU2 isoform, all of the mismatches being ... shownthat the catalytic reaction of GmGSTU4-4 is barelysensitive to the nature of the leaving group, as the sub-stitution of the chlorine atom with the more electro-negative ﬂuorine in the CDNB...

Báo cáo khoa học: Critical roles of Leu99 and Leu115 at the heme distal side in auto-oxidation and the redox potential of a hemeregulated phosphodiesterase from Escherichia coli pptx

... the ligand binding kinetics of the PAS domain of Ec DOS, as it does in myoglo-bin and hemoglobin. Examination of the effects of these hydrophobic amino acids on the kinetics of O2 and CO binding ... stableFe(II)–O2complex.CO binding kineticsWe next examined the kinetics of CO binding for the mutant proteins of Ec DosH using laser ﬂash photo-lysis (Fig. 6 and Table 4). The changes in the Soretregion of the absorption ... usedfor further determination of the physicochemical char-acteristics, such as the kinetics of O2or CO binding orFig. 2. Partial sequences of amino acids of the PAS domains of Ec DOS and other...

Báo cáo khoa học: Essential roles of lipoyl domains in the activated function and control of pyruvate dehydrogenase kinases and phosphatase isoform 1 pot

... running in opposite direc-tions. The monomer association involves the ATP-using C-terminal domains interacting near the base of that side of the wedge outside; the N-terminal domains form the ... acetyl-transferase-catalyzinginner domain. E3BP domains: L3, N-terminal lipoyl domain; B, E3binding domain; I, inner domain which associates with the innerdomain of E2. Dotted connections indicate binding interactions ... and E3 are included in Fig. 3. Apart from E2, the threedomain E3-binding protein (E3BP, Fig. 1) also has ananchoring C-terminal domain, an E3-binding domain and amobile lipoyl domain. Again,...

Báo cáo khoa học: Esculentin-1b(1–18) – a membrane-active antimicrobial peptide that synergizes with antibiotics and modiﬁes the expression level of a limited number of proteins in Escherichia coli doc

... (p0), the peptide (0.1–2 lm) was injectedinto the subphase, and the increment in surface pressure of the LPS ﬁlm upon intercalation of the peptide dissolved in the subphase was monitored for the ... 0.5 indicates synergy; see Experimentalprocedures]. To obtain insights into the mode of actionunderlying the synergistic activity, we investigated the bactericidal action of the combination of ... [20,48].Regardless of the precise mode of action, the effect(s) of antimicrobial peptides in general dependsupon their interaction with the microbial membrane[51,52]. In particular, the ﬁrst step in this...

Báo cáo khoa học: New roles of ﬂavoproteins in molecular cell biology: Blue-light active ﬂavoproteins studied by electron paramagnetic resonance pptx

... performedon the basis of the correlated coupled radical pairmodel and assuming the ﬁxed orientations of the Trp324•radical and the ﬂavin radical given by the 3Dstructure of the protein [86]. The ... are the absence of the signal from the H(5) pro-ton and the larger splittings of the signal pairs arisingfrom H(8a) and H(6) in the anion radical case. Hence, in addition to the g-tensor, the ... in the bindingpocket (and in the structural alignment of the twohistidines) upon substrate binding cannot be ruled out.As a second major result of the ENDOR study, the H(8a) ENDOR signal in...

Báo cáo khoa học: New roles of ﬂavoproteins in molecular cell biology: Histone demethylase LSD1 and chromatin pot

... discussed in the nextsection. The active site of all these amine oxidases is nor-mally located in the proximity of the flavin ring of the FAD cofactor and extends along the substrate-bindingdomain. ... domain organiza-tion in the first line. The third line shows a summary of the identified linear motifs (after filtering processing taking in account the accessibi-lity of the putative binding ... (interruptedby the sequence corresponding to the Tower domain that is in green in Fig 3A), whereas the SWIRM domain is in red. The second linereports the intrinsic protein disorder, domain and globularity...

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