Báo cáo khoa học: Analysis of Lsm1p and Lsm8p domains in the cellular localization of Lsm complexes in budding yeast ppt
Tài liệu Báo cáo khoa học: Oxidized elafin and trappin poorly inhibit the elastolytic activity of neutrophil elastase and proteinase 3 pdf
... half-life of dissociation of 36–105 min[15]. The oxidation of elafin and trappin down-shifts the half-life of there complexes with NE to % 1.3–1.8 min. On the other hand, the oxidized inhibitor–Pr3 complexes ... the theoretical inhibitor, regardless of the enzyme, the inhibitor and the state of oxidation of the latter, indi-cating that elastin does not simply act as a competingsubstrate but also hinders ... inhibitors.While the above data are in overall agreement with the results obtained using synthetic substrates, theyalso indicate that elastin hinders the inhibition of both enzymes by the native and the...
- 11
- 548
- 0
Tài liệu Báo cáo khoa học: Studies on structural and functional divergence among seven WhiB proteins of Mycobacterium tuberculosis H37Rv pdf
... forresistance to intracellular killing, the scavenging of freeradicals and the reactivation of degenerated proteinsduring infection by a family of proteins named ‘thio-redoxins’ is one of the elegant ... [16,26].Therefore, we assume that in WhiB proteins, one disul-fide bond is formed between the two cysteines of the CXXC motif (CXXXC in the case of WhiB5) and the other between the remaining two ... [2Fe–2S]cluster in each of the WhiB proteins, which remains stably bound to the proteins in the presence of 8 m urea. The [2Fe–2S] cluster of each proteinwas oxidation labile but the rate of cluster...
- 18
- 548
- 0
Báo cáo khoa học: Apolipoproteins A-I and A-II are potentially important effectors of innate immunity in the teleost fish Cyprinus carpio pot
... another nonimmune protein, histone H2A, in catfish skin, where a complex cascade of injury-inducedproteases is involved in the regulation of the A MP parasin Iproduction [5]. Therefore further ... a band in the range of m olecular m asses expected for the peptide ( 3 kDa).However with the antiserum against intact apoA-I weobserved during the first minutes of digestion a very faintband ... protein. The detection of the larger fragment (band b) of apoA-I in Fig. 4B indicates that it still contains at least part of the epitope(s) r ecognized by the antipeptide antibodies.Using the...
- 7
- 397
- 0
Tài liệu Báo cáo khoa học: Top-down MS, a powerful complement to the high capabilities of proteolysis proteomics pdf
... in the center of the protein to formadditional loose ends to be denatured out of the ball of spaghetti. The ESI spectrum of the 200 kDa human comple-ment C4 glycoprotein (of 1714 residues in ... mass increase with increasing time of deamidation(pH 9.6), the best fit of calculated intensity values(squares) was determined (Fig. 9B–E). The correspond-ing mass increase values in the ECD and ... cleavagescorresponding to the N-terminal sequence of the pre-dicted protein; the cleavage loss of the signal peptideleft two more amino acids on the protein than pre-dicted. Even if the bottom-up...
- 13
- 572
- 0
Báo cáo khoa học: Amino acids Thr56 and Thr58 are not essential for elongation factor 2 function in yeast potx
... of wild-type eEF2 thereby reducing the rate of proteinsynthesis and the growth rate of the transformed cells.As shown in Fig. 3B, the doubling rate was only mar-ginally affected by the presence of a nonfunctionaleEF2. ... protein (MAP) kinase and mTOR-signal-ling pathways [17]. These signalling pathways activate the eEF2 kinase in response to mitogens and otherstimuli that increase the cellular energy demand ... expression levels and ribosomal binding of tagged eEF2 analysed in the absence of competingeEF2 coded for by the yeast genome. As shown in Role of Thr56 and Thr58 for eEF2 function in yeast G. Bartish...
- 13
- 424
- 0
Báo cáo khoa học: Nautilin-63, a novel acidic glycoprotein from the shell nacre of Nautilus macromphalus doc
... control of the crystal orientation [21].On the other hand, the b- and c-axes of the nacretablets are oriented in parallel to the growing front in bivalves, whereas this is not the case in gastropods ... found both in the soluble and insoluble proteins, rendering it diffi-cult to classify according to the standard view on nacre proteins.Database The protein sequences of N63 appear on the UniProt ... BiocellInternational, Cardiff, UK). The size of the gold particleswas increased further by incubating the shell fragments in asilver-enhancing solution. The samples were then dried, and carbon...
- 14
- 383
- 0
Báo cáo khoa học: Par j 1 and Par j 2, the two major allergens in Parietaria judaica, bind preferentially to monoacylated negative lipids potx
... proteins (Fig. 7). Thus, the addition of a reduc-ing agent appears to be essential for comparing the thermostability of these proteins and the effect of OLPC binding. Par j proteins contain eight ... considered to be the functionallyrelevant moieties. Therefore, the character of the sidechains lining the cavities of Par j 1 and Par j 2 couldprovide more revealing insights into the proteins func-tion ... j 1 and Par j 2 proteins are the two major allergens in Parietaria juda-ica pollen, one of the main causes of allergic diseases in the Mediterraneanarea. Each of them contains eight cysteine...
- 14
- 440
- 0
Báo cáo khoa học: Analysis of Lsm1p and Lsm8p domains in the cellular localization of Lsm complexes in budding yeast ppt
... signals The N- and C-terminal extensions of Lsm1 p and Lsm8 p were fused to the N- or C-terminus of GFP,respectively, in order to test whether they containABFig. 2. Lsm1 p and Lsm8 p mutant and hybrid ... modification of the Lsm4 pC-terminus in response to stress.Although apparently important for the specificsubcellular localization of Lsm complexes, the N- orC-terminal domains of Lsm8 p and Lsm1 p are ... possibility that the Lsm1 p and Lsm8 p N-termini prevent aggregation of the Lsm complexes, potentially by interacting with the prion-like C-terminal domain of Lsm4 p. This asparagine-richregion of Lsm4 p...
- 16
- 515
- 0
Tài liệu Báo cáo khoa học: Analysis of proteins and peptides on a chromatographic timescale by electron-transfer dissociation MS ppt
... The small 30S, or S, subunit contains 21 proteins involved in mRNA binding. The 50S, or L, subunit contains 34proteins, binds to tRNA, and mediates peptidyl trans-fer. Shown in Fig. 4A is the ... mass spectrum of protein [M + 11H]+11ions at m ⁄ z 490.3 in (B). The observed ions of type c¢ and z¢Ædefine sequences at the N-terminus and C-terminus of the protein, respectively.These sequences ... residues near the C-terminus of the protein.As a result, the ETD spectrum is devoid of low massfragments of type z¢Æthat would provide sequenceinformation at the C-terminus of the protein. Althoughthis...
- 8
- 578
- 0
Tài liệu Báo cáo khoa học: Analysis of the molecular dynamics of medaka nuage proteins by fluorescence correlation spectroscopy and fluorescence recovery after photobleaching doc
... eggs in the one-cell stage to visualize the localization and to mea-sure the mobility of the components in the PGCs. The medaka embryo was peeled off the chorion, and the segment containing the ... diagram of the constructs microinjected into the medaka eggs and transfected into HeLa cells. The olvas, nanos and tudorcoding sequences were joined to the C-terminal region of the GFP or RFP coding ... mesoderm 0 min 1 min 2 min 3 min 4 min 9 min BAFig. 2. Time-lapse LSM analysis of Olvas–GFP reveals the dynamicnature of the nuage. Schematic diagram of the preparation of PGCs of medaka specimen...
- 9
- 655
- 0
Từ khóa: Một số giải pháp nâng cao chất lượng streaming thích ứng video trên nền giao thức HTTPNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDEPhối hợp giữa phòng văn hóa và thông tin với phòng giáo dục và đào tạo trong việc tuyên truyền, giáo dục, vận động xây dựng nông thôn mới huyện thanh thủy, tỉnh phú thọPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhPhát hiện xâm nhập dựa trên thuật toán k meansNghiên cứu tổng hợp các oxit hỗn hợp kích thƣớc nanomet ce 0 75 zr0 25o2 , ce 0 5 zr0 5o2 và khảo sát hoạt tính quang xúc tác của chúngNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Định tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Tìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinChuong 2 nhận dạng rui roKiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)Tranh tụng tại phiên tòa hình sự sơ thẩm theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn xét xử của các Tòa án quân sự Quân khu (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtNguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtMÔN TRUYỀN THÔNG MARKETING TÍCH HỢPQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ