Báo cáo khoa học: Simultaneous improvement of catalytic activity and thermal stability of tyrosine phenol-lyase by directed evolution ppt

... Simultaneous improvement of catalytic activity and thermal stability of tyrosine phenol-lyase by directed evolution Eugene Rha1, Sujin Kim1, Su-Lim ... mutations fromboth the stability and activity mutants, and demon-strated a signiﬁcant improvement in both stability and catalytic activity (Fig. 1). As such, the activity and sta-bility mutation ... reassembly–screening steps, therebyallowing the directed co -evolution of the stability and catalytic activity of S. toebii TPL.Materials and methodsMaterialsSodium pyruvate and PLP were obtained from...

Tài liệu Báo cáo khoa học: TEC family kinases in health and disease – loss-of-function of BTK and ITK and the gain-of-function fusions ITK–SYK and BTK–SYK pptx

... Approximately one-third of all mutations in the BTK gene are missense and some of these reduce the stability of the protein.This is exempliﬁed by mutations in the BTK motif of the Tec homology (TH) ... ancient and constitutes the second largest family of cyto-plasmic tyrosine kinases. In 1993, loss -of- function mutations in the BTKgene were reported as the cause of X-linked agammaglobulinemia. Of ... loss -of- function of BTK and ITK and the gain -of- function fusions ITK–SYK and BTK–SYKAlamdar Hussain1,2,*, Liang Yu1,3,*, Rani Faryal1,2, Dara K. Mohammad1, Abdalla J. Mohamed1,4 and C. I....

Tài liệu Báo cáo khoa học: Competition between innate multidrug resistance and intracellular binding of rhodamine dyes pdf

... efflux of rhodamine 123 and TMRM was inhib-ited by NBD-Cl and MK571 and, to a lesser extent, by ATP depletion (Fig. 6).An alternative experimental approach to demon-strate the uptake of rhodamine ... prevented by an active efflux pump. The efflux was inhibited by 4-chloro-7-nitro-2,1,3-benzoxadiazole (NBD-Cl) and MK571 and, to a lesser extent, by ATP depletion, indomethacin, probenecid and vanadate. ... linestested and the efflux of rhodamines in MRP1 over-expressing cells wasabolished by NBD-Cl, it appears that rhodamines are excluded from thesecells by MRP1. On the other hand, the uptake of rhodamines...

Tài liệu Báo cáo khoa học: A facile method for expression and puriﬁcation of the Alzheimer’s disease-associated amyloid b-peptide pdf

... E22K, E22Q and D23N), and the availability of a rapid and simpleexpression and purification protocol will facilitatelarge-scale investigations of the molecular determinants of aggregation and fibrillation. ... suppliedwith the enzyme, and contained Aba, Abb and Abcat40 nm each, and the start and stop primers Abstart and Abstop at 600 nm each, and 200 lm each of dATP, dCTP,dGTP and dTTP. The product ... cost of the purification, and the require-ment for HPLC increases the length and complexity of the purification procedure. Moreover, as with thestudies by Lee et al. [21] and Subramanian and Shree[42],...

Báo cáo khoa học: Human telomeric G-quadruplex: thermodynamic and kinetic studies of telomeric quadruplex stability potx

... consists of several kilobases of tandem repeats of the sequence 5¢-TTAGGG, includ-ing a terminal single-stranded overhang of $ 200nucleotides. This overhang can fold into a variety of quadruplex ... the stability of tethered and freesequences.Kinetics of human telomere quadruplexfoldingFormation of tetramolecular quadruplex structures isfourth order with respect to strand association and ... formed by human telomeric DNA and RNA. Na+ and K+cations appear to dictate the unimolecular folding of the telomeric DNA sequence into different forms,along with the exact sequences of the strand...

Báo cáo khoa học: A study on genomic distribution and sequence features of human long inverted repeats reveals species-speciﬁc intronic inverted repeats pptx

... stem.Studies of inverted repeats show that they may raiseinstability in a genome and, on the other hand, regu-late gene expression in both prokaryotes and eukary-otes. Being capable of forming ... Thestem-loops and palindromes constructed by invertedrepeats are involved in RNA interference, transcriptioninitiation of genes, initiation of DNA replication and alternative splicing of exons. ... essential for thedevelopment of the advanced functions of humannervous system in light of the Gene Ontology (GO)proﬁle of human orthologs.ResultsCharacters and distribution of LIRs in humanautosomesWe...

Báo cáo khoa học: Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding pptx

... Horˇejsˇı´3 and Jan Konvalinka1,21Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague, the Czech Republic;2Department of Biochemistry, Faculty of Natural ... catalytic domain (domain E) and the Ddomain of rat GCPII. The contribution of domains C and Fto the GCPII hydrolytic activity/ inhibitor binding remainsto be established.The 3D structure of ... chro-matography and chromatofocusing, as described previously[21].ResultsExpression and secretion of truncated variants of GCPIITo analyze the contribution of individual domains of human GCPII...

Báo cáo khoa học: Residues affecting the chloride regulation and substrate selectivity of the angiotensin-converting enzymes (ACE and ACE2) identiﬁed by site-directed mutagenesis pot

... atherosclerosis and acute lung injury. Both ACE and ACE2 have catalytic activity that is chloride sensitive and is caused by thepresence of the CL1 and CL2 chloride-binding sites in ACE and the CL1site ... I by tACE. (B) Cleavage of angiotensin I by ACE2. (C)Cleavage of angiotensin II by ACE2. Generation of product wasdetermined by HPLC. Values are the mean of triplicate determina-tions ± standard ... IIhydrolysis by enhancing the maximal level of activity, but not by altering the substrate-binding afﬁnity.Effects of CL1 and CL2 site mutations on thechloride sensitivity of tACE and ACE2 inhibitionIt...

Báo cáo khoa học: Puriﬁcation, characterization, cDNA cloning and nucleotide sequencing of a cellulase from the yellow-spotted longicorn beetle, Psacothea hilaris ppt

... detected three proteins bands by CBBstaining and one activity band by activity staining. Proteinsincluding cellulase activity were eluted and concentratedfrom the unstained gel, and the protein solution ... a number of proteinsfrom the acetone-treated samples were detected by CBBstaining, only one band was detected by activity staining(data not shown). After elution and concentration of theprotein ... were stained with CBB, and cellulase was visualized by activity staining on the gel. Inthe case of activity staining, the gel contained 0.1% (w/v)CMC and the conditions of sample treatment for...

Báo cáo khoa học: Structural insight into the evolutionary and pharmacologic homology of glutamate carboxypeptidases II and III ppt

... design of GCPIII-speciﬁc therapeutics and ligands, as well as inthe investigation of the diverse biological functions of GCPIII and GCPII.Experimental proceduresrhGCPIII and rhGCPII expression and ... Source, Argonne National Laboratory. Theuse of the Advanced Photon Source was supported by the US Department of Energy, Of ce of Science, Of ce of Basic Energy Sciences, under Contract No.W-31-109-Eng38. ... part by the Intramural Research Program of the NIH,National Cancer Institute, Center for Cancer Research(J.L. and C.B.). J.K. and K.H. were supported in part by the Ministry of Education of the...

Xem thêm