Báo cáo khoa học: Haptoglobin binds the antiatherogenic protein apolipoprotein E – impairment of apolipoprotein E stimulation of both lecithin:cholesterol acyltransferase activity and cholesterol uptake by hepatocytes pdf
... 2009 FEBS 6171
Haptoglobin binds the antiatherogenic protein
apolipoprotein E – impairment of apolipoprotein E
stimulation of both lecithin :cholesterol acyltransferase
activity and cholesterol uptake ... or
Hpt-free culture, respectively. After incubation, the cell were lysed
for measurement of their radioactivity and protein concentration...
... membranes
(Fig. 1A, lane 5).
It has been hypothesized that LDMs, which resemble the
inner envelope with regard to their lipid composition,
represent the sites of thylakoid membrane protein synthesis
and ... We
were able to show that RBP63 is a stromal thylakoid
membrane protein. It preferentially binds to the 5¢ UTR of
the psbA message determined by an A-rich region eight...
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Fig. 2. Sp1 binds to the p73 P1 promoter both in vitro and in vivo. (A) The
32
P-labelled region A target was incubated with the in vitro
Sp1 ... were performed as a negative control of interference. The experiment was performed in triplicate. (E) TAp73 levels w...
... were used to
complement the DnirF strain, they showed the same
phenotype as when complementation was done with
wild-type NirF. There were no growth defects and nei-
ther of these strains showed ... stoichiometric,
i .e. 1 mol of heme was taken up by 1 mol of NirF
(concentrations of heme and protein were calculated
by using the extinction coefficient mentioned in the
e...
... between these
two deletion strains is the absence of ISP in the first
strain compared to the second. However, a huge differ-
ence is seen in the molecular mass of the bc
1
complex in
these two deletion ... postulated the existence of distinct
sub-complexes in yeast mitochondria [12,2 4–2 7]. How-
ever, it is uncertain whether these sub-complexes repre-
sent true bc
1
assemb...
... transcription ter-
mination factor-related protein that is able to restore
thermotolerance, not only in the presence of the
Hsp101 allele used for the screen but also of the null
allele and other heat-sensitive ... (specialized median petal) of the flowers of the orchid Ophrys speculum closely resembles
the female wasp Colpa aurea, thus males of the species are at...
... determined by sequence analysis. X, unidentified residues. (C) The sequence of a putative 8K-BLP precursor polypeptide revealed
by an expressed sequence tag database search. The highlighted sequence ... Although recent analyses
of invertebrate genomes have revealed the presence of multiple insulin fam-
ily peptide genes in each genome, little is known about functional diversifi-...
... the intermediary enzymes (Fig. 1). The
overall arrangement of the tree is that the clusters of
true rBAT and 4F2hc proteins are separated from each
other by the GH13 enzymes.
All remaining sequences ... as the sequence similarities and differences
between the hcHAT proteins and GH13 enzymes are
concerned, the basic feature discriminating the 4F2hc
proteins from...
... con-
ducted before the techniques of molecular biology were
available, precluding the definitive identification of the
enzyme.
Another difference between vertebrate and inverte-
brate ChEs is that vertebrates ... including
the origins of the acyl pocket, the T exon, and the
asymmetric forms of ChE in the vertebrates.
Results
The sequence of the ChE from C. inte...
... due to protein precipitation. This
discrepancy could be due to the different experimental
conditions. Whereas the fluorescence decreases were
measured in situ, the remaining activities were deter-
mined ... collagen or tripalmitin as
standards. The experimental data were normalized to the
intensity of the incident beam and corrected for the detec-
tor response, the buffer...