... of HIV-1 capsid
FEBS Journal 276 (2009) 6118–6127 ª 2009 The Authors Journal compilation ª 2009 FEBS 6127
MINIREVIEW
The capsid protein of human immunodeficiency
virus: interactions of HIV-1 capsid ... 2009)
doi:10.1111/j.1742-4658.2009.07315.x
HIV-1 is a retrovirus that causes AIDS in humans. The RNA genome of
the virus encodes a Gag polyprotein, which is...
... but not with the D105G variant of
rMASP-2 and the Y59A variant of rMAp19, is consis-
tent with the fact that the variants lack the ability to
associate with rMBL [56,57]. In the same way, the fact
that ... [24–31]. These affect the oligomer-
ization of the protein, indirectly affecting the associa-
tion with the MASPs, or directly affecting the binding
s...
... stimulation, at either 1 h or 3 h (supple-
mentary Fig. S1). As qRT-PCR could not exclude the
possibility of release of pre-existing IL-6, we further
monitored the secretion of IL-6 protein by ELISA.
ISO ... could not increase IL-6 protein production as
compared with control within 3 h in the medium of
cultured cardiomyocytes (supplementary Fig. S2).
Therefore, there may b...
... independent
proteins and peptides leads to the disassembly of the
spherical capsid inside the virion. Some of the folded
domains of Gag are then able to reassemble as inde-
pendent proteins. The matrix protein ... diverse CA–CA interactions and inter-
faces involved either in the assembly of the immature
HIV-1 capsid, or in the reassembly of CA into a
mature...
... 2009)
doi:10.1111/j.1742-4658.2009.07314.x
The mature capsid of human immunodeficiency virus, HIV-1, is formed by
the assembly of copies of a capsid protein (CA). The C-terminal domain of
CA, CTD, is able to homodimerize and most of the ... Mascarenhas AP & Karin Musier-Forsyth K (2009)
The capsid protein of human immunodeficiency virus:
interacti...
... forms of the
MAPKs were produced as GST-lofMPK3 and GST-
lofMPK6. However, when lofMPK3 and lofMPK6
proteins were tested for phosphorylation with OXI1,
no phosphorylation of lofMPK3 or lofMPK6 ... mixed with GST alone as a
control or with GST-tagged proteins. (B) The HIS-PTI1-4 protein was mixed with GST alone as a control or with GST-tagged proteins. In (A)
and (B), the...
... [10].
Although the occurrence of family 33 CBPs has been
known for some time [23], the present results provide
only the third demonstration of the accessory function
of these proteins. The effect of LlCBP33A ... drawn
for ChiC1 from S. marcescens. The fact that the longer
part of the substrate extends towards the + side of the
catalytic centre is compatible wi...
... aB-crystallin (i.e. the effect of
the additive on the aggregation of the target protein is inde-
pendent and different from that of the chaperone). The
standard errors associated with this theoretical ... (see
below). The effect of the additives on aggregation of the
target protein (in the absence and presence of aB-crystallin)
was assessed at the end...
... represents the co-operativity of the transition, and [D]
50%
is the concentration of urea at the midpoint of unfolding, i.e.
the concentration of urea required to denature 50% of the protein. Intersection ... [33,34].
Discussion
According to the prion hypothesis, a key molecular
event in the pathogenesis of prion diseases is the
conversion of the normal cell...
... 17% of residues within this stretch.
We replaced the critical tri-glycine within the pea
Toc75 transit peptide with repeats of these three
Fig. 3. Time course of the distribution of imported proteins. ... specificity in vitro.
Replacement of the most C-terminal segment with alanine residues resulted
in mistargeting the protein to the stroma, while exchange of eith...