Báo cáo khoa học: Expression of cholinesterases in human kidney and its variation in renal cell carcinoma types pdf

... Cholinesterases in renal carcinomasFEBS Journal 277 (2010) 4519–4529 ª 2010 The Authors Journal compilation ª 2010 FEBS 4529 Expression of cholinesterases in human kidney and its variation in ... renal cholinesterases are still lacking. To ﬁll the gap and todetermine whether cholinesterases are abnormally expressed in renal tumours, paired pieces of normal kidney and renal cell carcinomas ... extent of binding with thelectins concanavalin A, Lens culinaris agglutinin(LCA), and Ricinus communis agglutinin (RCA)(Fig. 3), ruled out the blood origin and supported the renal cells themselves...

Báo cáo khoa học: Expression of CYP2E1 increases oxidative stress and induces apoptosis of cardiomyocytes in transgenic mice pdf

... Peking Union Medical College, Beijing, China2 Key Laboratory of Human Diseases Animal Model, State Administration of Traditional Chinese Medicine, Institute of Laboratory AnimalScience, Chinese ... The overexpression of CYP2E1 both in vivo and in vitro is associated with several cellular markers of oxidative stress, including products of lipid peroxi-dation (e.g. 4-hydroxynonenal and malondialdehyde),as ... isoforms. Overexpression of CYP2E1 is of direct importance to human health and has been associated with a range of diseases, includingdiabetes [1–3], alcoholic liver disease and cancer [4–8]. In addition,...

Báo cáo khoa học: Ubiquitination of E3 ubiquitin ligase TRIM5a and its potential role pdf

... TRIM5aubiquitinates itself through the function of its RING-finger domain in HEK293T cells.Ro52 strongly ubiquitinates itself and TRIM5a in HEK293T cellsTRIM5a functions as an E3 ubiquitin ligase, ... TRIM5a and its RING mutant in vitro. (A) Schematic representation of the RING-finger domain of TRIM5a.The amino acid sequence and structure of the RING-finger domain are shown. Asterisks indicate ... as a RING-finger-type E3 ubiquitin ligase both in vitro and in vivo and ubiquitinates itself in cooperation with the E2 ubiquitin-conjugatingenzyme UbcH5B. In addition to the self-ubiquitination,...

Báo cáo khoa học: "Stochastic Language Generation Using WIDL-expressions and its Application in Machine Translation and Summarization" pot

... states and in exists if there exists a vertex in the description of and a vertex in the de-scription of such that there exists a path in between and , and is the only -labeledtransitions in this ... WIDL -expression in Figure 1.WIDL-graphs have an initial vertex and a ﬁnalvertex . Vertices , , and with in- goingedges labeled , , and , respectively, and vertices , , and with out-going edges la-beled ... WIDL-speciﬁctraversal of , starting from and ending in .Each path (and its associated string) has a proba-bility value induced by the probability distributionfunctions associated with the edge labels of ....

Báo cáo Y học: Complexation of ytterbium to human transferrin and its uptake by K562 cells pot

... Lu3+,Sm3+ and Eu3+,respect-ively [34]. Both UV and ICP-AES data suggested that twoYb3+bind to apo-Tf in the specific iron binding site and twotyrosines are involved in binding of Yb3+ in both ... collected with Band Pass (BP) filter and LongPass (LP) 590 nm filter.RESULTSBinding of Yb3+to apo-hTfThe thermodynamics of binding of Yb3+to apoTf.Binding of Yb3+to apo-Tf was investigated ... Fe3+.Interestingly, the binding to the N-lobe is kineticallyfavored. The binding of Yb2-Tf to K562 cell membranes(TfR) was demonstrated and was weaker than that of Fe2-Tf and only part of...

Tài liệu Báo cáo khoa học: Expression of poly(A)-binding protein is upregulated during recovery from heat shock in HeLa cells doc

... lg of plasmid DNAusing 10 lg of lipofectamine (Invitrogen, Burlington,Canada), according to protocols supplied by the manufac-turer. In short, the DNA was mixed with lipofectamine and PABP expression ... basis of ligand recognition by PABC, a highly speciﬁc peptide-binding domain found in poly(A)-binding protein and aHECT ubiquitin ligase. EMBO J 23, 272–281.9 Craig A (1998) Interaction of polyadenylate-bindingprotein ... that, in addition to the C-terminal domain, PABP1has another eIF4G-binding domain within its N-termi-nal RNA-binding domain 1 [27]. As a negative control,we also examined the colocalization of...

Tài liệu Báo cáo khoa học: Expression of two [Fe]-hydrogenases in Chlamydomonas reinhardtii under anaerobic conditions doc

... rabbits to induce antibody generation. Theresulting serum was immunoaﬃnity puriﬁed and tested forreaction against HydA1 and HydA2 overexpressed in E. coli and against HydA2 in anaerobically induced ... reinhardtii HydA1 and HydA2) upstream of the H-cluster motif 1 and (b) asecond amino acid insertion (16 residues in C. fusca,16 residues in S. obliquus HydA1 and HydA2, 45 residues in C. reinhardtii ... differences in the regulation of expression of the two genes.Gene expression during anaerobic inductionThe coexpression of the HydA1 and HydA2 transcripts wasstudied by Northern blot analysis. In Fig....

Báo cáo khóa học: Characterization of presenilin complexes from mouse and human brain using Blue Native gel electrophoresis reveals high expression in embryonic brain and minimal change in complex mobility with pathogenic presenilin mutations pptx

... immunoreactivityandrevealedstrongdetectionofa10-kDabandinPS1+/+neural stem cells, embryonic brain, and SY5Y cells; lowerlevels were detected in PS1–/– cells, and in adult brain frommouse and human (Fig. ... 10-kDa band in PS1 wild-typeneural stem cells, E15 mouse brain and SY5Y cells. Low-level expression was present in PS1 deﬁcient cells, adult mouse brain and in control and AD human brain cortex ... Tris-glycine SDS/PAGE of 20 lg membrane samples of human brain analysed in (A) indicated no major diﬀerence in PS1 or NCT expression in AD. (D) Analysis of samples used in (B) for PS1 and NCT expression...

Báo cáo khoa học: Expression of the pyrG gene determines the pool sizes of CTP and dCTP in Lactococcus lactis doc

... in pyrimidine metabolism, as the enzymecatalyses the only reaction resulting in the amination of the pyrimidine ring into a cytosine derivative (Fig. 1). It istherefore of interest to examine ... present in M17 medium inhibits cytidine metabo-lism, most likely by inhibiting the uptake of cytidine [15].Adding cytidine in excess at 1000 lgÆmL)1relieves theinhibitory effect and allows ... °ConSAplatescontaining glucose, erythromycin and cytidine. Severalcolonies from the plates were inoculated in 1 mL SAmedium with glucose, erythromycin and cytidine and grownfor 5–6 h at 30 °C. This growing...

Báo cáo khoa học: Expression of the Pycnoporus cinnabarinus laccase gene in Aspergillus niger and characterization of the recombinant enzyme pdf

... mgÆL)1 of wild-type laccase from thedikaryotic strain I-937 of P. cinnabarinus and 145 mgÆL)1from the derived monokaryotic strain ss3 of P. cinnabarinus. In order to improve the secretion of the ... determination.RESULTSTransformation and screening In a cotransformation experiment, A. niger D1 5#26 wastransformedwithamixtureofplasmidpAB4-1andeachofthe t wo expression vectors c ontaining ... loadingdifference. As seen in Fig. 2B, production of laccase bypLac1-B increased until day 1 2. This is also supported bycontinuous level of expression of the recombinant lac1transcripts during...

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