Báo cáo khoa học: Staphylococcus aureus elongation factor G – structure and analysis of a target for fusidic acid pdf

... 3803 Staphylococcus aureus elongation factor G – structure and analysis of a target for fusidic acid Yang Chen, Ravi Kiran Koripella, Suparna Sanyal and Maria SelmerDepartment of Cell and Molecular Biology, ... The mutations can be classiﬁed as affecting FA binding, EF -G ribosome interactions, EF -G conformation, and EF -G stability.AbbreviationsEF -G, elongation factor G; EF-Tu, elongation factor Tu; ... with domains G and III undergo largechanges between the free and ribosome-bound states of EF -G, and thus the same mutations can also affectthe conformational dynamics of EF -G. Gly617 and Gly621,...

Tài liệu Báo cáo khoa học: An anthrax lethal factor mutant that is defective at causing pyroptosis retains proapoptotic activity pdf

... An anthrax lethal factor mutant that is defective atcausing pyroptosis retains proapoptotic activityStephanie Ngai, Sarah Batty, Kuo-Chieh Liao and Jeremy MogridgeDepartment of Laboratory ... inducing vascular leakage that leadsto shock and multiorgan failure [ 3–6 ]. The role of LeTx in anthrax pathogenesis is complex, however, and probably involves the impairment of the innate and adaptive ... Jose, CA, USA). Antibodies raised againstthe N-termini of MAPKK3b (catalog no. 9238), MAPKK4(catalog no. 9152) and MAPKK7 (catalog no. 4172) wereobtained from Cell Signaling Technologies. Antibodies...

Tài liệu Báo cáo khoa học: The Ets transcription factor ESE-1 mediates induction of the COX-2 gene by LPS in monocytes doc

... 5¢-CAGTCTTATAAAAACCAAGGTTCTCTCGGTTAGCGACC-3¢; (c) COX-2 promoterEts site #3, 5¢-GACGAAATGACTGTTTCTTTGAGCCTTTTCGTACCCC-3¢; (d) COX-2 promoter Ets site #4,5¢-AGGGGAGAGGAGGGTTAAATTTGTGGGGGGTACGAAAAGGCGG-3¢; (e) ... following primers were used (the mutated bases areunderscored): (a) COX-2 promoter Ets site #1, 5¢-GCTGAGGAGTAGCTGGACGTGCTCCTGAC-3¢;(b)COX-2promoter Ets site #2, 5¢-CAGTCTTATAAAAACCAAGGTTCTCTCGGTTAGCGACC-3¢; ... #5:5¢-GGGTTTTTTACCCACGCTAATGAGAAAATCGGAAACC-3¢.DNA transfection assaysCotransfections were carried out in 6-well plates containing 3–8 · 105cells per well using 600 ng of reporter gene con-struct...

Tài liệu Báo cáo khoa học: An alternative isomerohydrolase in the retinal Muller cells of a cone-dominant species doc

... GATATCTTATGGTTTGTACATCCCATGGAAAGRPE65c-FwdNM_001113653 GCGGCCGCCACCATGGTCAGCCGTCTTGAACACRPE65c-Rev AAGCTTCTAAGGTTTGTAGATGCCGTGGAGRPE6 5a GSP-FwdNM_200751 TGGGGAGGACTTTTATGCTGTRPE6 5a GSP-Rev CTTTTGTGTAGGTGGGATTCG13cIMH ... CTTTTGTGTAGGTGGGATTCG13cIMH GSP-FwdNM_001089433 CTGAGGTTACAGACAACTGTTC13cIMH GSP-Rev CCTTTGACATCGCAAGTGGATCARPE65c GSP-FwdNM_001113653 TTGAGGTGACAGACAATTGCCTRPE65c GSP-Rev TCTTTGACTTCTCAAACTGATCGRPE6 5a- His-FwdNM_200751 ... TCTTTGACTTCTCAAACTGATCGRPE6 5a- His-FwdNM_200751 GCGGCCGCCACCATGCATCATCACCATCACCATGTCAGCCGTTTTGAACACRPE65c-His-FwdNM_001113653 GCGGCCGCCACCATGCATCATCACCATCACCATGTCAGCCGTCTTGAACACY. Takahashi et al. A novel isomerohydrolase...

Tài liệu Báo cáo khoa học: "Finding Synonyms Using Automatic Word Alignment and Measures of Distributional Similarity" pdf

... in a source language and a target language given sen-tence aligned parallel corpora. We applied stan-dard settings of the GIZA++ system without anyoptimisation for our particular input. We also ... parallelcorpora. It seems that languages that are similarto the target language, for example in word or-der, are good candidates for ﬁnding synonyms athigh precision rates. Also the fact that ... 11 languages in parallel, it is sentencealigned, and it is of reasonable size. Thus, for acquiring Dutch synonyms we have 10 languagepairs with Dutch as the source language. TheDutch part...

Báo cáo khoa học: Relation between domain evolution, speciﬁcity, and taxonomy of the a-amylase family members containing a C-terminal starch-binding domain pot

... hydrolases (mainly a- amylases) and transglycosidases (principally CGTases)was not readily deﬁned, because maltogenic a- amylase,acarviose transferase, and the archaeal CGTase clusteredtogether at a distance ... similar and contain two maingroups of enzymes, an amylase group and a CGTasegroup. The tree for domain C changed substantially, theseparation between the amylase and CGTase groups beingshortened, ... glucoamylase; Horre GAM, Hormoconis resinae glucoamylase; Humgr GAM, Humicola grisea glucoamylase.Fig. 1. Stereo view of a CGTase as an example of a ﬁve-domain member of the a- amylase family having the C-terminal...

Báo cáo khoa học: N-glycosylation inﬂuences the structure and self-association abilities of recombinant nucleolin pot

... primers 5¢-CAATCTCTTTGTTGGAAACCTAAACTTTCAGAAATCTGCTCCTGAATTAAAAACTGG-3¢ and 5¢-CCAGTTTTTAATTCAGGAGCAGATTTCTGAAAGTTTAGGTTTCCAACAAAGAGATTG-3¢. Mutation of Asn492 (N > A) was achie-ved with ... primers 5¢-GGTGAATCAAAAACTCTGGTTTTAAGCCAGCTCTCCTACAGTGCAACAGAAGAAACTC-3¢ and 5¢-GAGTTTCTTCTGTTGCACTGTAGGAGAGCTGGCTTAAAACCAGAGTTTTTGATTCACC-3¢.The coding region of nucleolin cDNAs corresponding tothe ... 5¢ -G CCAAACAGAAAGCAGCTCCTGGATCCAAGAAACA G- 3¢ and 5¢-GTGCCTTCCACTTTCTGTTTCTTGGATCCAGGAGCTGCTTTC-3¢. After sequence veriﬁcation, theBamH1- and EcoR1-digested inserts were cloned into sim-ilarly...

Báo cáo khoa học: Key substrate recognition residues in the active site of a plant cytochrome P450, CYP73A1 ppt

... 5¢-TCCGTATGGCGGCTCCGCTTCTAGTC-3¢ 5¢-GACTAGAAGCGGAGCCGCCATACGGA-3¢ 58I371K 5¢-TCCGTATGGCGAAACCGCTTCTAGTC-3¢ 5¢-GACTAGAAGCGGTTTCGCCATACGGA-3¢ 58K484M 5¢-GATACCGATGAGATGGGTGGGCAGTTTAG-3¢ 5¢-CTAAACTGCCCACCCATCTCATCGGTATC-3¢ ... 5¢-CAACATTCCTTGTCATCGAACCAAACTC-3¢ 58R103M 5¢-GTTCGAGAACAATGAATGTTGTGTTC-3¢ 5¢-GAACACAACATTCATTGTTCTCGAAC-3¢ 55R103E 5¢-GAACACAACATTCTCTGTTCTCGAACC-3¢ 5¢-GGTTCGAGAACAGAGAATGTTGTGTTC-3¢ 55DKR 5¢-GAAGTTAAAGATACAATGATTCAGCTC ... 5¢-CGGGATCGCCATTTCGAGACGGAGGG-3¢ 50R368F 5¢-CCCTCCGTCTCTTTATGGCGATCCG-3¢ 5¢-CGGGATCGCCATAAAGAGACGGAGGG-3¢ 50I371F 5¢-TCCGTATGGCGTTCCCGCTTCTAGTC-3¢ 5¢-GACTAGAAGCGGGAACGCCATACGGA-3¢ 58I37 1A 5¢-TCCGTATGGCGGCTCCGCTTCTAGTC-3¢...

Báo cáo khoa học: "Efﬁcient Minimum Error Rate Training and Minimum Bayes-Risk Decoding for Translation Hypergraphs and Lattices" pptx

... multi-language case, we trainphrase-based systems and perform lattice MBR for all language pairs. We use a scaling factor of 0.7 for all pairs. Additional gains can be obtainedby tuning this factor; ... and Minimum Bayes-Risk Decoding for Translation Hypergraphs and LatticesShankar Kumar1 and Wolfgang Macherey1 and Chris Dyer2 and Franz Och11Google Inc.1600 Amphitheatre Pkwy.Mountain View, CA ... of arity 1 is a regular edge, and a hypergraph of arity 1 is a regu-lar graph (lattice). Each hyperedge is labeled with a rule refrom the SCFG. The number of nontermi-nals on the right-hand...

Báo cáo khoa học: From meiosis to postmeiotic events: Alignment and recognition of homologous chromosomes in meiosis ppt

... [1]), and mechanisms for DNA strand exchange have been determined at atomicresolution (W. Kagawa and H. Kurumizaka, this issue[2]). However, before a pair of homologous DNAstrands can interact ... in a mutant lacking Spo11 and other keyfactors for DSB formation and recombination of DNA,some residual levels of pairing still remain, suggestingthat a DSB-independent pairing mechanism may ... attached to a speciﬁctranscription factory, in which transcriptionalmachinery proteins are aggregated, and those DNAregions that are not undergoing transcription pro-trude from the factory and...

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