... transcription–PCR analysis of unknown serial analysis of geneexpression tags; rSAGE, reverse serial analysis of gene expression; SAGE, serial analysis of gene expression; TSAT-PCR, two-step analysis of unknown ... Semi-nested PCR analysis of unknown tags on serial analysis of gene expressionWang-Jie Xu1, Qiao-Li Li1, Chen-Jiang Yao1, Zhao-Xia Wang1, Yang-Xing Zhao1andZhong-Dong Qiao1,21 ... study of related data. In this study, in order to identify agene, we developed a semi-nested PCR-based method called the two-step analysis of unknown SAGE tags (TSAT-PCR) to generate longer 3¢-endcDNA...