... alpha-sarcin: a study of relationships. Meth Enzymol 341,335–351.3 Lacadena J, A ´lvarez-Garcı´ a E, Carreras-Sangra N,Herrero-Gala´n E, Alegre-Cebollada J, Garcı´ a- OrtegaL, On˜aderra ... were analyzed in a semi-automated iterative mannerby cyana 2.1 [38]. The NOE coordinates and intensitiesused as input for automated analysis were generated auto-matically by Sparky based on the ... con-formation in which it is pointing towards the negatively charged D40. This position should favor a salt bridge interaction that will decrease the pK a of the aspartic acid and increase the pK a of...