Báo cáo khoa học: A new phospholipase A2 isolated from the sea anemone Urticina crassicornis – its primary structure and phylogenetic classification pptx
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A new phospholipase A
2
isolated from the sea anemone
Urticina crassicornis – its primary structure and
phylogenetic classification
Andrej Razpotnik
1
, Igor Kriz
ˇ
aj
2
, Jernej S
ˇ
ribar
2
, ... we report
the isolation and characterization of a PLA
2
from the northern Pacific sea
anemone, Urticina crassicornis (UcPLA
2
), containing...
... tachykinin-related peptide
Atsuhiro Kanda, Kyoko Takuwa-Kuroda, Masato Aoyama and Honoo Satake
Suntory Institute for Bioorganic Research, Osaka, Japan
Tachykinins (TKs) are vertebrate multifunctional brain ... Lys–Pro–Pro–Ser–Ser–Ser–Glu–Phe–Ile–Gly–Leu–Met–NH
2
oct-TK-II Lys–Pro–Pro–Ser–Ser–Ser–Glu–Phe–Val–Gly–Leu–Met–NH
2
Substance P and SP-(Arg11)
Substance P Arg-Pro-Lys-Pro-Gln-Gln-Ph...
... redistributed from the ER
membrane to form ‘punctae’ and aggregates near the
plasma membrane [1,6]. The N-terminal region of
STIM1 contains a canonical EF-hand motif and a pre-
dicted SAM domain. Stathopulos ... compact shape. Thus, the [Ca
2+
]
changes in the ER lumen are sensed by the canonical
EF-hand motif and cause conformational changes in
this motif. The Ca
2+...
... occidentalis
and Pyura vittata); 11 species of Cnidaria (Bartholo-
mea annulata, Budonosoma granulifera, Cassiopea
xamachana, Condylactys gigantea, Gorgonia ventalina,
Lebrunia danae, Palythoa caribaeorum, ... the kingdom Methazoa
(Phyla: Annelida, Urochordata, Echinodermata, Cnidaria,
Mollusca, Artropoda) were collected in the north coast of
Havana and classified by Cuban specialists...
... purpose, an EcoRI
site immediately adjacent to the stop codon was created by
PCR using the Pfu DNA polymerase, the forward primer
S64XHOF 5¢-AAGAAAC
TCGAGGTCGAAGA-3¢
(coordinates 10 3–1 21, XhoI ... to the isolation of its
cDNA from an expression library prepared from cotyledon
mRNA [9]. Molecular characterization of the cDNA-
encoded product revealed that SBP was quite diss...
... human iPLA
2
-VIA antibody were purchased from
Cayman Chemical (Ann Arbor, MI, USA). Pyrrophenone
was kindly provided by T. Ono (Shionogi Research
Laboratories, Osaka, Japan). All other reagents ... The natural membrane assay was
carried out exactly as described above except that CaCl
2
was
omitted and 5 mm EDTA was added instead. All of these
assay conditions have been validated previo...
... [70,71]. Goat anti-human
COX-1 and anti-human COX-2, rabbit anti-human group
IVA cPLA
2
a, and goat anti-human caveolin-2 (sc-1858)
were purchased from Santa Cruz (Santa Cruz, CA, USA).
Rabbit antibodies ... is also distributed rather specifically in
VSMC of other organs, such as heart, mammary
gland, gastrointestinal tract and male reproductive
organs (S Masuda, M Murakami, Y Ishikaw...
... that treating AA as a text classifica-
tion problem is viable, but the feature types are all
fairly shallow, and the approach doesn’t make effi-
cient use of the training data as a separate classifier
is ... a head and dependent in a grammatical re-
lation (GR) from the RASP GR output, calculated
for each GR graph from the top 10 parses per sen-
tence. In particular, we...
... cocktail (Roche) and 5 mm b-mercapto-
ethanol. The lysate was sonicated, then centrifuged. The
supernatant solution was loaded into Ni-nitrilotriacetic acid
agarose (Qiagen, Valencia, CA, USA), and ... FP, VFP, was isolated and cloned from the
C. microphthalma coral, collected in 1.2 m of water off
Lizard Island on the Australian Great Barrier Reef
[25,26]. The alignment o...
... 110, 95 6–9 64.
10 Tsukuba T, Hori H, Azuma T, Takahashi T, Taggart
RT, Akamine A, Ezaki M, Nakanishi H, Sakai H &
Yamamoto K (1993) Isolation and characterization of
recombinant human cathepsin ... activity
CaD activity
TAPA
CatE activity
CaD activity
TAPA
CatE activity
CaD activity
A
B
C
Fig. 5. Distribution of TAPA, CatE and CatD activity in subcellular
fractions of the cell...