Báo cáo khoa học: Crystal structure of salt-tolerant glutaminase from Micrococcus luteus K-3 in the presence and absence of its product L-glutamate and its activator Tris pdf
... at its N-terminus
instead of a free amino group [32]. Therefore, the
asparagine in position 26 was labeled within the intact
protein at its a-amino group and represents the amino
terminal amino ... the amino terminus of the pro-
tein. Using this method, we showed that asparagine 26
represents the amino terminus of P40. Knowing, that
the first amino acids of the...
... its native structure after the inser-
tion of foreign sequences and in the presence and
absence of Ca
2+
ions, so that the in uence from the
host protein to the inserted sites is minimized [14]. ... was introduced to perturb the Ca
2+
-binding ability of the grafted EF-hand of STIM1.
(B) Modelled structure of the engineered protein with the graft...
... form the phenolic substrate-binding site of the
enzyme, whereas the T2 and the T3-pair coppers form
the trinuclear site that is responsible for binding and
reduction of the molecular oxygen. The ... which is Asp in TaLcc1, and Glu in MaL. In addi-
tion, the crystal structure of TaLcc1 does not have a chloride attached to
the T2 copper, as observed in...
... determined the X-ray crystal structure of a truncated form
of importin-a lacking the importin-b binding domain, bound to a CLIC4
NLS peptide. The NLS peptide binds to the major binding site in an
extended ... cargo binding sites, referred to as
the major and minor binding sites [1]. These sites are
located in the concave face of the protein near regions
of inva...
... an independent metal-binding site
at the interface of the two domains, which consists
of four side chains: two (His31 and His79) from the
N-terminal domain and two (Asp165 and His169) from
the ... residues
involved in the binding of the metal cofactor.
ApeSODs in different forms were superimposed with the
least square fit of Ca atoms of the residues in...
... is the only direct contact
between the protein backbone and the substrate. The
side chain of Thr292 is found to recognize the 2-phos-
phate in both the binding model of PhyK and the crys-
tal structure ... in the
acidic range. They consist of two domains, a large a ⁄ b
domain and a small a domain with the catalytic site at
the interface of the two dom...
... on the
remaining subgroups of the HAT ⁄ HAT-like ⁄ DESC
and corin subfamilies exists. We therefore cloned,
expressed and purified the serine proteinase domain of
DESC1 and solved the crystal structure ... cystein-rich
(SRCR) domain, which was rigidly bound to the back
of the proteinase domain in the crevice between the
C-terminal helix, the 204-loop and t...
... role in the enzymatic
reaction which will be discussed later.
The structure of the active site, the type of protein
ligands, the zinc-binding motif, the presence of a water
Fig. 3. Structure of the ... residue in the zinc-
bound MshB structure (PDB code 1Q74) adopts a sim-
ilar strained conformation. On the other hand, in the
absence of a zinc ion...
... surfa-
ces. Furthermore, the substrate-binding cleft of 1SH7
differs from that of 1IC6 and 1THM in shape, being
seemingly deeper and more distinct, and in being more
negatively charged than the binding ... ongoing mutagenic research in
which single and combined amino acid substitutions
aimed at increasing the stability of the Vibrio protein-
ase are being tested....
... sequences and superimpos-
able 3D structures [24]. Parallel studies of these effects
allowed identification of the specific interactions between
the inhibitors and the proteins. In the absence of a 3D
structure ... the
phosphorylation step, Arg249 may induce compression of
the product, to set it on its way for expulsion from the active
site (or its introdu...