Báo cáo khoa học: Crystal structure of salt-tolerant glutaminase from Micrococcus luteus K-3 in the presence and absence of its product L-glutamate and its activator Tris pdf

Tài liệu Báo cáo khoa học: Experimental proof for a signal peptidase I like activity in Mycoplasma pneumoniae, but absence of a gene encoding a conserved bacterial type I SPase pdf

Tài liệu Báo cáo khoa học: Experimental proof for a signal peptidase I like activity in Mycoplasma pneumoniae, but absence of a gene encoding a conserved bacterial type I SPase pdf

... at its N-terminus instead of a free amino group [32]. Therefore, the asparagine in position 26 was labeled within the intact protein at its a-amino group and represents the amino terminal amino ... the amino terminus of the pro- tein. Using this method, we showed that asparagine 26 represents the amino terminus of P40. Knowing, that the first amino acids of the...
Ngày tải lên : 19/02/2014, 18:20
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Báo cáo khoa học: A single EF-hand isolated from STIM1 forms dimer in the absence and presence of Ca2+ ppt

Báo cáo khoa học: A single EF-hand isolated from STIM1 forms dimer in the absence and presence of Ca2+ ppt

... its native structure after the inser- tion of foreign sequences and in the presence and absence of Ca 2+ ions, so that the in uence from the host protein to the inserted sites is minimized [14]. ... was introduced to perturb the Ca 2+ -binding ability of the grafted EF-hand of STIM1. (B) Modelled structure of the engineered protein with the graft...
Ngày tải lên : 07/03/2014, 00:20
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Tài liệu Báo cáo khoa học: Crystal structure of an ascomycete fungal laccase from Thielavia arenaria – common structural features of asco-laccases ppt

Tài liệu Báo cáo khoa học: Crystal structure of an ascomycete fungal laccase from Thielavia arenaria – common structural features of asco-laccases ppt

... form the phenolic substrate-binding site of the enzyme, whereas the T2 and the T3-pair coppers form the trinuclear site that is responsible for binding and reduction of the molecular oxygen. The ... which is Asp in TaLcc1, and Glu in MaL. In addi- tion, the crystal structure of TaLcc1 does not have a chloride attached to the T2 copper, as observed in...
Ngày tải lên : 14/02/2014, 18:20
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Tài liệu Báo cáo khoa học: Crystal structure of importin-a bound to a peptide bearing the nuclear localisation signal from chloride intracellular channel protein 4 ppt

Tài liệu Báo cáo khoa học: Crystal structure of importin-a bound to a peptide bearing the nuclear localisation signal from chloride intracellular channel protein 4 ppt

... determined the X-ray crystal structure of a truncated form of importin-a lacking the importin-b binding domain, bound to a CLIC4 NLS peptide. The NLS peptide binds to the major binding site in an extended ... cargo binding sites, referred to as the major and minor binding sites [1]. These sites are located in the concave face of the protein near regions of inva...
Ngày tải lên : 14/02/2014, 19:20
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Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf

Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf

... an independent metal-binding site at the interface of the two domains, which consists of four side chains: two (His31 and His79) from the N-terminal domain and two (Asp165 and His169) from the ... residues involved in the binding of the metal cofactor. ApeSODs in different forms were superimposed with the least square fit of Ca atoms of the residues in...
Ngày tải lên : 14/02/2014, 22:20
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Tài liệu Báo cáo khoa học: Crystal structure of Klebsiella sp. ASR1 phytase suggests substrate binding to a preformed active site that meets the requirements of a plant rhizosphere enzyme doc

Tài liệu Báo cáo khoa học: Crystal structure of Klebsiella sp. ASR1 phytase suggests substrate binding to a preformed active site that meets the requirements of a plant rhizosphere enzyme doc

... is the only direct contact between the protein backbone and the substrate. The side chain of Thr292 is found to recognize the 2-phos- phate in both the binding model of PhyK and the crys- tal structure ... in the acidic range. They consist of two domains, a large a ⁄ b domain and a small a domain with the catalytic site at the interface of the two dom...
Ngày tải lên : 16/02/2014, 09:20
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Tài liệu Báo cáo khoa học: Crystal structure of the catalytic domain of DESC1, a new member of the type II transmembrane serine proteinase family pptx

Tài liệu Báo cáo khoa học: Crystal structure of the catalytic domain of DESC1, a new member of the type II transmembrane serine proteinase family pptx

... on the remaining subgroups of the HAT ⁄ HAT-like ⁄ DESC and corin subfamilies exists. We therefore cloned, expressed and purified the serine proteinase domain of DESC1 and solved the crystal structure ... cystein-rich (SRCR) domain, which was rigidly bound to the back of the proteinase domain in the crevice between the C-terminal helix, the 204-loop and t...
Ngày tải lên : 19/02/2014, 00:20
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Tài liệu Báo cáo khoa học: Crystal structure of the BcZBP, a zinc-binding protein from Bacillus cereus doc

Tài liệu Báo cáo khoa học: Crystal structure of the BcZBP, a zinc-binding protein from Bacillus cereus doc

... role in the enzymatic reaction which will be discussed later. The structure of the active site, the type of protein ligands, the zinc-binding motif, the presence of a water Fig. 3. Structure of the ... residue in the zinc- bound MshB structure (PDB code 1Q74) adopts a sim- ilar strained conformation. On the other hand, in the absence of a zinc ion...
Ngày tải lên : 19/02/2014, 00:20
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Tài liệu Báo cáo khoa học: Crystal structure of a subtilisin-like serine proteinase from a psychrotrophic Vibrio species reveals structural aspects of cold adaptation docx

Tài liệu Báo cáo khoa học: Crystal structure of a subtilisin-like serine proteinase from a psychrotrophic Vibrio species reveals structural aspects of cold adaptation docx

... surfa- ces. Furthermore, the substrate-binding cleft of 1SH7 differs from that of 1IC6 and 1THM in shape, being seemingly deeper and more distinct, and in being more negatively charged than the binding ... ongoing mutagenic research in which single and combined amino acid substitutions aimed at increasing the stability of the Vibrio protein- ase are being tested....
Ngày tải lên : 19/02/2014, 16:20
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Tài liệu Báo cáo khoa học: Crystal structure of Trypanosoma cruzi glyceraldehyde-3-phosphate dehydrogenase complexed with an analogue of 1,3-bisphospho-D-glyceric acid Selective inhibition by structure-based design docx

Tài liệu Báo cáo khoa học: Crystal structure of Trypanosoma cruzi glyceraldehyde-3-phosphate dehydrogenase complexed with an analogue of 1,3-bisphospho-D-glyceric acid Selective inhibition by structure-based design docx

... sequences and superimpos- able 3D structures [24]. Parallel studies of these effects allowed identification of the specific interactions between the inhibitors and the proteins. In the absence of a 3D structure ... the phosphorylation step, Arg249 may induce compression of the product, to set it on its way for expulsion from the active site (or its introdu...
Ngày tải lên : 20/02/2014, 02:21
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