Báo cáo khoa học: Lysophosphatidylcholine modulates ﬁbril formation of amyloid beta peptide doc

... Lysophosphatidylcholine modulates fibril formation of amyloid beta peptide Abdullah Md. Sheikh and Atsushi NagaiDepartment of Laboratory Medicine, Shimane University School of Medicine, ... of transmembrane amyloid precursor protein [1]. Bio-chemical analysis of the Ab peptides isolated from ADKeywordsAlzheimer’s disease; amyloid beta peptide; fibril formation; lysophosphatidylcholine; phospholipid–Ab ... 2010)doi:10.1111/j.1742-4658.2010.07984.xPhospholipids are known to influence fibril formation of amyloid beta (Ab) peptide. Here, we show that lysophosphatidylcholine (LPC), a polar phos-pholipid, enhances Ab(1-42) fibril formation, by decreasing...

Báo cáo khoa học: Stability and ﬁbril formation properties of human and ﬁsh transthyretin, and of the Escherichia coli transthyretin-related protein potx

... fibril formation of piscine transthyretin: therole of the N-terminal. Mol Cell Endocrinol 295(1-2),48–58.34 Santos SD, Costa R, Teixeira PF, Gottesman M,Cardoso I & Saraiva MJ (2008) Amyloidogenicproperties ... Stability and fibril formation of TTR and TRPFEBS Journal 276 (2009) 1999–2011 ª 2009 The Authors Journal compilation ª 2009 FEBS 2011Stability and fibril formation properties of human andfish ... pointmutations, of which more than 80 have been identified,cause FAP and FAC [6]. TTR mutations associatedwith familial amyloid diseases display a wide range of Keywords amyloid; fibril formation; ...

Báo cáo khoa học: Lpe10p modulates the activity of the Mrs2p-based yeast mitochondrial Mg2+channel pot

... Mg2+-dependent inﬂux. High copy number expression of Lpe10p led to an increased rate of uptake of Mg2+upon addition of 1 and 3 mm [Mg2+]e. Expression of Mrs2–Lpe10p or Lpe10–Mrs2p chimeric proteins ... coexpression of Mrs2p-Myc (Fig. 6B, upperpicture). Incubation of the same blot with anti-mycserum resulted in identiﬁcation of Mrs2p-Myc-contain-ing complexes of high molecular mass, which were of similar ... X-100concentration of the supernatant was reduced to 0.8%. Onehundred microliters of Protein A Dynabeads (Invitrogen,Lofer, Austria) was washed with solubilization buffer +0.8% Triton X-100. Coating of the...

Báo cáo khoa học: Solubility-dependent structural formation of a 25-residue, natively unfolded protein, induced by addition of a seven-residue peptide fragment pot

... dependence of the structural stabilities of FPs. (A) Inte-grated intensities of long-range NOE peaks of FP1. (B) Integratedintensities of short- and medium-range NOE peaks of FP1. (C) Frac-tions of ... solvent.Mode of formation of loosely packed structurethrough intermolecular interactionsWhen the hydrophobic peptide fragment of FIV-VALG was added to the C-terminus of unstructuredIP consisting of ... structures of FP1,assuming that long-range restraints excluded in theABFig. 3. pH dependence of the solubilities of FPs. (A) Experimen-tally obtained plots of the natural logarithm of the solubility,...

Tài liệu Báo cáo khoa học: Active and regulatory sites of cytosolic 5¢-nucleotidase doc

... transfer of phosphate esteriﬁed in the 5¢ position of 6-hydroxypu-rine monophosphate nucleosides [1]. The transfer of phosphate can lead to phosphorylation of inosine,guanosine and a number of their ... for the survival of several cell types. cN-IIcatalyses both the hydrolysis of nucleotides and transfer of their phosphatemoiety to a nucleoside acceptor through formation of a covalent phospho-intermediate. ... ﬁrst of the fourconserved motifs [13]. Formation of a phospho-inter-mediate suggests the possibility that the enzyme cataly-ses transfer of phosphate to a suitable acceptor [15].A number of...

Tài liệu Báo cáo khoa học: Structural bases for recognition of Anp32⁄LANP proteins doc

... sub-unit of PP2A as modeled by comparison with the U2A¢–U2B¢¢complex. The other two subunits shield most of the surface of PP2A–C. (B) Structure of the U2A¢–U2B¢¢ complex [20].Study of the interactions ... Theidentiﬁcation of phosphorylation sites of pp32 and bio-chemical puriﬁcation of a cellular pp32-kinase. Biochem-istry 43, 10157–10165.27 Bairoch A (1992) PROSITE: a dictionary of sites andpatterns ... is a member of the Anp32 family of acidicnuclear evolutionarily-conserved phosphoproteins,which present a broad range of activities [1]. They arecharacterized by the presence of a highly conservedN-terminal...

Tài liệu Báo cáo khoa học: Steady-state kinetic behaviour of functioning-dependent structures docx

... rate of transformation of S1into S3is calculated as corresponding to both the rate of consumption of S1, v(s1), and the rate of production of S3, v(s3), and the shape of the curves ... here.ResultsKinetics of the overall reaction of transformation of S1into S3The system with only the free enzymes, E1and E2The overall rate of functioning of two free sequentialenzymes of the Michaelis–Menten ... concentration, s1,ofS1, the absolutevalues (positive) of the steady-state rate of transforma-tion of S1into S3is calculated as corresponding toboth the rate of consumption of S1, v(s1),...

Tài liệu Báo cáo khoa học: Bioinformatic and enzymatic characterization of the MAPEG superfamily doc

... set of sequences of all superfamilymembers. To also visualise the more general relationships of the families included in MAPEG an unrooted consensustree was produced. The consensus sequences of ... (1996) Sequence analysis of the gen-ome of the unicellular cyanobacterium Synechocystis sp.strain PCC6803. II. Sequence determination of theentire genome and assignment of potential protein-cod-ing ... origin of these forms dates back to beforethe occurrence of vertebrates, i.e. more than 500 mya.This dates the differentiation of the MAPEG formsback to the late Cambrian multiplicity of eukaryoticspecies....

Tài liệu Báo cáo khoa học: "Subjectivity and Sentiment Analysis of Modern Standard Arabic" doc

... native speakers of Arabic annotated 2855 sentences from Part1 V 3.0 of the PATB. The sentences make upthe first 400 documents of that part of PATBamounting to a total of 54.5% of the PATBPart ... contacting the first author.587 of a sentence is judged as the objective reporting of information, it was labeled as OBJ. Otherwise, asentence would be a candidate for one of the threeSUBJ classes. ... expression of opinions, evaluations, feelings, and speculations(Banfield, 1982; Wiebe, 1994) and thus incorporatessentiment. The process of subjectivity classificationrefers to the task of classifying...

Tài liệu Báo cáo khoa học: "A Finite-State Model of Human Sentence Processing" docx

... composed of featureslike POS, the number of complements, category of each complement, and the position of comple-ments. In their view, structural disambiguationis simply another type of lexical ... adequate account of recur-sive syntactic structure is an essential component of any model of the behaviour. In this study, wetested a bigram POS tagger on different types of structural ambiguities ... pair of sentences. Comparatively faster or larger drop of probability is expected to be a good indicator of comparative processing difﬁculty. Probability re-ranking, which is a simpliﬁed model of...

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