Báo cáo khoa học: Increased expression of c-Fos by extracellular signal-regulated kinase activation under sustained oxidative stress elicits BimEL upregulation and hepatocyte apoptosis pot
... Increased expression of c-Fos by extracellular
signal-regulated kinase activation under sustained
oxidative stress elicits BimEL upregulation and hepatocyte
apoptosis
Yasuhiro ... reactive
oxygen species and apoptosis in rat primary hepatocytes. Apoptosis was
accompanied by increased expression of BimEL, following activation of
extrace...
... the expression of Rb and Sp1.
Fig. 1. Effects of UCN-01 on the levels of Rb and Sp1 protein. (A), (B) Dose–response of the effect of UCN-01 on the levels of retinoblastoma gene
product (Rb) and ... Rb and Sp1
protein caused by CDK inhibitors in CHO cells
The increase in total levels of Rb and Sp1 proteins caused by
UCN-01 may be a result of the enhanced transcrip...
... de-
phosphorylation of phosphorylase-a and activation of
glycogen synthase [28]. Overexpression of PTG caused
inactivation of phosphorylase (Fig. 4A), activation of
glycogen synthase and stimulation of glycogen ... synthesis and glucokinase
activity was determined by overexpression of gluco-
kinase with varying titres of recombinant adenovirus.
Although glycogen...
... fraction. In-gel digestion of the R2F band
and protein identification by Q-TOF MS-MS was performed
as described previously [50].
Preparation of the native R2F-protein
For enrichment of R2F from C. ammoniagenes ... vitro, and 1 mm MgCl
2
in 85 mm potassium phosphate
buffer (pH 6.6). The reaction was started by addition of the
catalytically active holoenzyme for 5 min of incuba...
... or
excess of kinetic energy (as a measure of temperature)
and its effect on enzyme activity.
Alternatively, we know of a number of examples of
qualitative strategies, for example, when expression of
distinctly ... activity) of an enzyme can be chan-
ged by affecting rates of transcription and ⁄ or transla-
tion and ⁄ or mRNA and protein degradation. This
represents...
... chamber under a light micro-
scope.
Measurement of oxidative stress and DNA
content
Oxidative stress was measured through the detection of
ROS and of protein-bound acrolein. ROS produced within
HepG2 ... Interestingly,
increased expression of CD1d molecules by iron-loaded HepG2 cells was
associated with increased phosphatidylserine expression in the outer leafle...
... presence of turnover of the
protein. Quantitation of the Mcm4 band suggested that the
protein stability of Mcm4 does not greatly differ between
HeLa and WI-38 cells (Fig. 5C).
Expression of Mcm3 and ... of Mcm expression in normal human fibroblasts from
umbilical cord (HUC-F cells) and WI-38 cells.
Expression of the human
Mcm2
gene
To understand the mechanism behind t...
... differential activation of the
various Drosophila heat shock factor (HSF) isoforms
[54] or from incomplete activation of HSFs under cold
stress, as observed under mild heat stress [55].
Upregulation of ... 9 h of
cold stress at 0 °C and during 8 h of recovery at 25 °C. Increased expres-
sion of some, but not all, Hsp genes was elicited in response to cold stres...
... Heremans
2
and Remko M. Boom
1
1 Food and Bioprocess Engineering Group, Department of Agrotechnology and Food Sciences, Wageningen University and Research Centre,
The Netherlands
2 Department of Chemistry, ... that inactivation of the dimeric
almond b-glucosidase was not a result of unfolding,
dissociation or aggregation of the intact enzyme [16].
Influence of temperature o...
... gene under the control of the GAL10 pro-
moter. The induction of the yeast, by galactose containing
media, results in overexpression of GAL4, in turn inducing
an increase of the expression of the ... Identifica-
tion of ligands for olfactory receptors by functional
expression of a receptor library. Cell 95, 917–926.
20 Hatt H, Lang K & Gisselmann G (2001) Functional...