... of1mLÆmin)1, with a linear gradient from 100% B to 43% Bwithin 45 min, and then to 0% within 1 min, with the finalconditions being maintained for another 24 min at a flowrate of 2 mLÆmin)1. ... trans-formant strains were named T3 and T4.The molecular integrity of carD was investigated in T3 and T4 strains, in two nonsectoring transformants,T1 and T2, and in the SF134 original strain. ... domain is shaded in gray. The TMdomain of YLO-1 is shaded in black, and theequivalent sequence in CarD is boxed. Thetwo amino acid changes found in thisprotein segment in F. graminearum CarD(FGSG09960,...