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Sixth Conference
of the
European Chapter
of the
Association for
Computational Linguistics
Proceedings of the Conference
21 - 23 April 1993
9~~ OTS - Research Institute
for Language ...
This volume contains the papers prepared for the Sixth Conference of the European Chapter
of the Association for Computational Linguistics, held...
... Professor Hayao Taguchi (Tokyo
University of Science) for the gift of the plasmid carry-
ing the LDH gene from T. caldophilus; Dr Miho
Aoshima and Ms Ayako Nishiyama for the preparation
of the ... that
H. thermophilus POR functions toward pyruvate syn-
thesis in vivo.
To investigate the reaction mechanism of H. thermo-
philus POR, we measured the EPR spectra of th...
... assembly of for-
oxymithine by a synthetase lacking all E-domains. The
lack of sequence information for the S. nitrosporeus
genome impeded the identification of a biosynthetic
machinery governing foroxymithine ... to the identification of the second siderophore
(data not shown). We therefore assume that either
the extraction conditions were inadequate for the iso-
la...
... synthesis of pantothe-
nate from low amounts of pantoate [9]. Here, we
report the results of the structural studies of the panto-
ate-bound form of the N-terminal domain of E. coli
PS (nPS). The ... occupy allowed regions of the Rama-
chandran map [32,33]. Comparison of the monomer of
the substrate-free form of E. coli PS [13] with the
monomers of nPS i...
...
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63
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THE Gim
Ban
chdt
cua cuoc khung hoang kinh te the
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chu nghTa xa hoi the ky XXI
DAO THE TUAN
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7" ien n...
... to the principles
laid down in the latest World Health Organization classifi-
cation. The investigation of these tissues was in accordance
with the rules of the Declaration of Helsinki and the
Ethical ... with 100 l
M CoCl
2
for 2 h and then with 10 lM forskolin for a
further 24 h. (C) Morphology of cells. (D) Immunocytochemistry of GFAP levels. (E) Quantification of...
... to, for example, a single substitution;
MS ⁄ MS will then show the substituent positions of dif-
ferent isomers. A problem for MS ⁄ MS of either the
peptides for the bottom-up approach or of the ... a molecular mass of 16 309.7 Da, but
this matched none of the DNA-predicted proteins.
MS ⁄ MS of these ions gave the C-terminal sequence of
Fig. 5. These and all ot...
... Effect of BEL on the growth of U937 cells. (A) Dose–
response curve for the effect of BEL on the Ca
2+
-independent
PLA
2
activity of U937 cell homogenates. The substrate was pre-
sented in the form ... Ca
2+
-independent
PLA
2
activity of U937 cell homogenates. The substrate was pre-
sented in the form of mixed micelles produced using Triton X-100.
(B) Time course...
... Thus, we confirmed that there was no
amplification of traces of the genomic DNA (data not
shown).
Expression of the cloned receptor in Xenopus
oocytes
The ORF region of the novel receptor cDNA ... designed on the
basis of the conserved regions, and were used for
RT-PCR of first-strand cDNA prepared from the
Octopus heart. blast searches of the PCR product
sequence...
... column
was exclusively used for purification of each mutant.
For purification of the 38-kDa mature form of the
E78H ⁄ D164N mutant, the crude extract of the E. coli
transformant cells (prepared with ... equili-
brated mixture of the nicked and intact forms of the precursor. X-ray crys-
tallographic structures of the mature forms of the two mutants showed
that...