... checked by 1HNMR and HPLC.Spectrophotometric determination of laccase activityThe activity of laccase was determined by following the rate of oxidation of 2,2¢-azinobis-(3-ethylbenzothiazoline-6-sulfonate) ... N-hydroxyphtalimide (HPI) and 2,2,6,6-tetramethylpiperidin-1-yloxy free radical (TEMPO) asmediators, since they greatly differ both in their mechan-ism of action and in their specificity. In fact, HPI isrepresentative ... oxidation by laccase, at least in terms of redox potential; (b) thenumber of repeat units in each oligomer, and therefore itssize, is exactly determined, because directed synthesis and Fig. 1. Catalytic...