Tài liệu Báo cáo Y học: Bivalent cations and amino-acid composition contribute to the thermostability of Bacillus licheniformis xylose isomerase doc

... amino-acid composition contribute to the thermostability of Bacillus licheniformis xylose isomerase Claire Vieille1, Kevin L. Epting2, Robert M. Kelly2 and J. Gregory Zeikus11Department of Biochemistry ... example,thermostable xylose isomerases (XIs) (EC 5.3.1.5), whichcatalyze the isomerization of D -xylose to D-xylulose in vivo,are used for the conversion of D-glucose into D-fructose for the production ... relatively low catalytic efﬁciency on xylose. BLXI thermostability and inactivation characteristicsBLXI thermostability was ﬁrst characterized using the holoenzyme in the presence of 1 mM CoCl2....

Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by ﬂuorescence spectroscopy pdf

... The phosphoryl and pyridoxyl moieties of the P-pyridoxyl group are shown in red and magenta, respectively. The fractional solventexposed area of the P-pyridoxyl group is 0.39 and 0.082 for the ... P-pyridoxyl group to the solvent. The fluorescence decay of pyridoxamine and of the P-pyridoxyl-labeled protein were monitored at 393 nmupon excitation at 326 nm. The emission decay of pyridox-amine ... anothersubstrate of the enzyme, also increased the P-pyridoxylfluorescence. The addition of this substrate to the proteinblue shifted the maximum by 5 nm and the fluorescenceFig. 3. Steady state...

Tài liệu Báo cáo Y học: Identiﬁcation and characterization of a mammalian 14-kDa phosphohistidine phosphatase pdf

... A˚keEngstro¨m at the Peptide Synthesis and Analysis Laboratory of the Department of Medical Biochemistry and Microbio-logy, Uppsala University. The SP6 primer 5¢-ATT TAGGTG ACA CTA TAG-3¢ and the three ... wasthus similar to that of the porcine liver enzyme. The specificity of the recombinant enzyme towardsphosphohistidine was supported by the finding that none of the eight O-phosphorylated peptides ... degree of dephosphory-lation was calculated from the peak areas of phosphorylated and dephosphorylated peptides. In a control experiment, the stoichiometry of the cleavage was confirmed by the occurrence...

Tài liệu Báo cáo Y học: Regulated expression and intracellular localization of cystatin F in human U937 cells pptx

... production of cystatin F compared to the ubiquitous inhibitor, cystatin C, we measured the cystatin contents in cell lysates and culture media of cellsgrown for 2 and 4 days with cystatin F and cystatin ... of the cystatin F gene and promoter. The structures of the human cystatin F (CST7)andcystatinC(CST3) genes are illustratedschematically at the top. In the upstream region of the CST7 gene, the ... regulated by naturalinhibitors belonging to the cystatin superfamily. Theseinhibitors are of three major types [1]. Those of type 1,cystatins A and B (also called steﬁns), are approximately100 amino-acid...

Tài liệu Báo cáo Y học: Structural diversity and transcription of class III peroxidases from Arabidopsis thaliana docx

... a nonglycosylated triplet at position Asn70 [48]. The majority of the peroxidases carry one or two putativeglycans. Seven appear to be nonglycosylated. Therefore, the high number of glycans found ... [64] have studied the promoteractivity and wound-induction of HRPC and -E types, and of AtPCa and AtPEa. Most remarkably, C2, and only C2,responded strongly to wounding. The AtP38 gene mightrespond ... used for the first strand synthesis. Primersspecific to each peroxidase gene were used for the secondstrand synthesis and PCR amplification (Supplementarymaterial, Table S1). The specificity of each...

Tài liệu Báo cáo Y học: A novel, inducible, citral lyase puriﬁed from spores of Penicillium digitatum docx

... as the standard.RESULTSInduction of citral lyaseInitially, the reproducibility of the results was hindered byvariations in the citral lyase activity of the P. digitatumspores. Remarkably, ... hydratase and aldolase activityin a single enzyme. No other enzyme has been reported to catalyse the conversion of citral into methylheptenone and acetaldehyde, or a similar conversion of othera,b-unsaturated ... is approximately the monomeric size of these hydratase/aldolase enzymes. All of these enzymesexhibit more then 75% of their maximum activity at pH 7.6, the optimum pH of citral lyase [27,30–32]....

Tài liệu Báo cáo Y học: Novel bradykinins and their precursor cDNAs from European yellow-bellied toad (Bombina variegata) skin potx

... endotheliallayer of the artery was removed by bubbling with oxygenfor 10 s. Absence of the endothelial layer was conﬁrmed by the lack of relaxation in response to a 30-min perfusion of acetylcholine ... This sensitivity is well within the capability of the ES-MS system employed. The primary structural diversity displayed withinamphibian skin bradykinins may not simply be due to phylogenetic differences ... standardization of each synthetic peptide wasachieved by acid hydrolysis of a known gravimetric quantity of lyophilisate followed by amino acid analysis using anApplied Biosystems PTH-amino acid analyser.cDNA...

Tài liệu Báo cáo Y học: Intracellular localization and transcriptional regulation of tumor necrosis factor (TNF) receptor-associated factor 4 (TRAF4) pdf

... localized in the cytoplasm (Fig. 6, middle panel). The GFP-tagged TRAF domains of TRAF2 and TRAF3also localized to the cytoplasm whereas the TRAFdomain of TRAF1 showed nuclear and cytoplasmiclocalization ... implicatedmainly in signaling by members of the TNF receptor family,TRAF6 primarily transduces signals initiated by IL1/Tollreceptors. In particular, TRAF4 has been shown to interactwith the lymphotoxin-b ... factor (TNF) receptor-associated factor(TRAF) family comprises a group of adaptor proteins thatare involved in signal transduction by members of the TNFreceptor and IL1/Toll-receptor family...

Tài liệu Báo cáo Y học: Phosphatidylinositol synthesis and exchange of the inositol head are catalysed by the single phosphatidylinositol synthase 1 from Arabidopsis docx

... hostnaturally devoid of phosphatidylinositol (PtdIns). In the context of the bacterial membrane and in addition to de novosynthesis, the plant enzyme is capable of catalysing the exchange of the inositol ... Phosphatidylinositol synthesis and exchange of the inositol headare catalysed by the single phosphatidylinositol synthase 1fromArabidopsisAnne-Marie Justin, Jean-Claude Kader and Sylvie CollinUniversite´Pierre ... showing that the endogenous level of CDP-DAG is too low to allow detectable synthesis. The level of PtdIns in the membranes does not allow synthesis of detectable PtdIns by exchange of the polar...

Tài liệu Báo cáo Y học: Identiﬁcation and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx

... previouslyshown that at the beginning of the stationary phase the rate of synthesis of heat-shock proteins increases considerably,but only transiently [2,18]. Similarly, we observed that the steady-state ... the deletion of the UP12 encoding gene increased the sensitivity of the mutant to CCCP (Fig. 6), it did not haveany detectable effects on the sensitivity to mitomycin C,unlike in the case of ... may represent true physiologically relevantinteractions in stationarily grown cells.Identiﬁcation of proteins X (GatY) and Y (UP12)In order to identify polypeptides X and Y, we repeated the experiment...

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