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Tài liệu Báo cáo Y học: Studies on the nonmevalonate pathway of terpene biosynthesis The role of 2C-methyl-D -erythritol 2,4-cyclodiphosphate in plants docx

Tài liệu Báo cáo Y học: Studies on the nonmevalonate pathway of terpene biosynthesis The role of 2C-methyl-D -erythritol 2,4-cyclodiphosphate in plants docx

Tài liệu Báo cáo Y học: Studies on the nonmevalonate pathway of terpene biosynthesis The role of 2C-methyl-D -erythritol 2,4-cyclodiphosphate in plants docx

... (Eur. J. Biochem. 268) q FEBS 2001 Studies on the nonmevalonate pathway of terpene biosynthesis The role of 2C-methyl-D-erythritol 2,4-cyclodiphosphate in plants Monika Fellermeier1, Maja Raschke1, ... (2000) Studies on the nonmevalonate pathway: conversion of 4-(cytidine 50-diphospho)-2-C-methyl-D-erythritol to its 2-phos-pho derivative by 4-(cytidine 50-diphospho)-2-C-methyl-D-erythri-tol ... were then extracted with ethyl acetate. The aqueous phase was analyzed by HPLC in order to monitor the conversion of [1-3H ]2C-methyl-D- erythritol 4-phosphateinto the corresponding 2,4-cyclodiphosphate. ...
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Tài liệu Báo cáo khoa học: Studies on structural and functional divergence among seven WhiB proteins of Mycobacterium tuberculosis H37Rv pdf

Tài liệu Báo cáo khoa học: Studies on structural and functional divergence among seven WhiB proteins of Mycobacterium tuberculosis H37Rv pdf

... proteins remains very high.Protein ligands for the canonical clusters are typi-cally sulfide ions of cysteines. The involvement of fourconserved cysteines in the coordination of the [4Fe–4S]cluster ... differentialelectronic environment. The nature and type of aminoacids and their side-chain orientations around iron–sulfur cluster coordination sites are the likely cause of minor variations in the electronic ... system.Although insulin is a non-natural substrate, the dif-ference in activity indicates that the efficiency of reduc-tion of target disulfides by WhiB proteins may vary in vivo. Moreover, the...
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Tài liệu Báo cáo Y học: Studies into factors contributing to substrate specificity of membrane-bound 3-ketoacyl-CoA synthases pot

Tài liệu Báo cáo Y học: Studies into factors contributing to substrate specificity of membrane-bound 3-ketoacyl-CoA synthases pot

... in the hydroxylases. The mutation of these residues in the desaturases to the corresponding hydroxylase residuesresulted in the conversion of desaturase activity to hydroxy-lase activity. The ... reflect the available substrates in this yeast system and/or the ability of the enzymes further down the metabolic pathway to use the products of the FAE1 KCS condensation activity. Indeed, in the ... activities of FAE1 KCSs. However, the results are reflective of the entireyeast metabolic pathways from the synthesis of 3-ketoacylCoAs by a condensing enzyme to the incorporation of fattyacids into...
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Tài liệu Báo cáo Y học: Bivalent cations and amino-acid composition contribute to the thermostability of Bacillus licheniformis xylose isomerase doc

Tài liệu Báo cáo Y học: Bivalent cations and amino-acid composition contribute to the thermostability of Bacillus licheniformis xylose isomerase doc

... BLXI on glucose and on xylose, the apoenzyme activity was tested on both sugars in the presence of increasing concentrations of each cation (in the chloride form; Fig. 4). In the absence of metal, ... Asboth the Ea of inactivation and the temperature at which the enzyme starts inactivating at a measurable rate increasefrom the apoenzyme to the Mn2þ-containing enzyme, loss of the metal cofactor ... example,one of the most evident patterns in the amino-acidcomposition of hyperthermophilic proteins is the biasagainst thermally labile amino-acid residues. This pattern isobvious on examination of...
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Tài liệu Báo cáo Y học: The glucose-specific carrier of the Escherichia coli phosphotransferase system Synthesis of selective inhibitors and inactivation studies pptx

Tài liệu Báo cáo Y học: The glucose-specific carrier of the Escherichia coli phosphotransferase system Synthesis of selective inhibitors and inactivation studies pptx

... wererecently introduced for the preparation of C-6 aldehydederivatives of Glc [15]. The key step is the use of Collinsreagent for the selective oxidation of the primary trimethyl-silyl ether of the ... (1986) The oxidation of primary trimethylsilyl ethers toaldehydes: a selective conversion of a primary hydroxy group intoan aldehyde group in the presence of a secondary hydroxy group.J. Prakt. ... thatdephosphorylation and/or catalytic turnover of EIIGlc,rather than binding of Glc, enhanced the reactivity of Cys421. As Cys421 is the only invariant cysteine in homologous transporters and also the...
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Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf

Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf

... synergy of enzymes degrading the pectin side chains was studied [34]. GALA had a positiveeffect on the activity of the other enzymes involved in the degradation of these side chains.DISCUSSIONA. ... The increase in expression levels of galA in the CREA mutant,indicates that at least one of the detected putative CREAbinding sites in the promoter of galA is functional.Hydrolysis of arabinogalactans ... mutant expression of galA was detected in the presence of D-galacturonic acid andL-arabinose. The expression of galA is very low on the inducingcompounds. Using approximately 50 ng of the PCRproduct...
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Tài liệu Báo cáo Y học: The RuvABC resolvasome Quantitative analysis of RuvA and RuvC assembly on junction DNA doc

Tài liệu Báo cáo Y học: The RuvABC resolvasome Quantitative analysis of RuvA and RuvC assembly on junction DNA doc

... suggest that the protein may nowbe binding to the duplex arms of the junction, as opposedto only the crossover points of the junction, suggestingthat the protein may no longer be binding in a structurespecific ... stoichiometry of the interaction of RuvAwith the Holliday junction, the biotinylated Holliday junc-tions were injected over the surface of the streptavidin coatedsensor chip and the change in response ... highlystructure specific in its binding to Holliday junctions. The effect of the charge on the central pin with respect to the specificity of the interaction was further investigated bySPR analysis...
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Tài liệu Báo cáo Y học: The effects of ring-size analogs of the antimicrobial peptide gramicidin S on phospholipid bilayer model membranes and on the growth of Acholeplasma laidlawii B ppt

Tài liệu Báo cáo Y học: The effects of ring-size analogs of the antimicrobial peptide gramicidin S on phospholipid bilayer model membranes and on the growth of Acholeplasma laidlawii B ppt

... peptide incorporation into Myr2Gro-PEtnbilayers. The initial DSC heating scans of MLVs of Myr2Gro-PGro alone, or Myr2Gro-PGro MLVs containing 4 molepercent of one of the three GS ring-size ... zwitterionicphospholipid bilayers studied here but a smaller effect in the anionic phospholipid bilayer system. The potency of the three ring-size analogs of GS in inducing the leakage of calcein dye ... the integrity of the lipid bilayer of the inner membraneis the primary mode of antimicrobial action of this peptide[8]. In support of this hypothesis, GS has been found tointeract strongly with...
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Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx

Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx

... support of this hypo-thesis are meager. In the head-to-tail orientation, the catalytic site in the N-terminal domain of one subunit and the substrate recognition site in the C-terminal domain of the ... peak, whereas the elution of the low-affinity peak was unchanged.To investigate the effect of substitutions in the tryptophancluster of the C-terminal domain on heparin affinity, the W393A/W394A ... (Arg405, Lys407,Lys413, Lys414, Lys422, Lys428, and Lys430) oriented in the same direction with the heparin-binding domain at the N-terminal end [14]. The other is a cluster of tryptophans(Trp390,...
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Tài liệu Báo cáo Y học: Targeting of malate synthase 1 to the peroxisomes of Saccharomyces cerevisiae cells depends on growth on oleic acid medium pptx

Tài liệu Báo cáo Y học: Targeting of malate synthase 1 to the peroxisomes of Saccharomyces cerevisiae cells depends on growth on oleic acid medium pptx

... dehydrogenase activity speci®cally involved in the glyoxylate cycle is attributed to the cytosolic isoformMdh2p [7]. The suggestion of an extra-peroxisomal locationfor the yeast g lyoxylate cycle ... model fungal system forstudying the glyoxylate cycle by analysing the subcellulardistribution of malate synthase 1. The S. cerevisiae glyoxylate cycle (Scheme 1) consists of ®ve enzymatic activities, ... similarity is curren tlybeing addressed.Demonstration of the cytosolic location of Mls1p in wild-type cells grown on ethanol completes the picture of the extra-peroxisomal location of the glyoxylate...
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